Support and reagent availability for HiSeq 3000 Systems ends February 28, 2023. Support and reagent availability for HiSeq 4000, certified used (CPO) HiSeq 4000 and cBot 2 Cluster generation Systems ends March 31, 2024. Contact us to see if you qualify for a trade–in.

HiSeq 3000/4000 SBS Kit

Includes sequencing reagents specifically designed for the HiSeq 3000/HiSeq 4000 Systems. Enables production-scale sequencing for diverse applications. Read More...
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HiSeq 3000/4000 SBS Kit (50 cycles)



HiSeq 3000/4000 SBS Kit (150 cycles)



HiSeq 3000/4000 SBS Kit (300 cycles)


Illumina Advantage Products

TG HiSeq® 3000/4000 SBS Kit (50 cycles)



TG HiSeq® 3000/4000 SBS Kit (150 cycles)



TG HiSeq® 3000/4000 SBS Kit (300 cycles)



TG HiSeq® 3000/4000 PE ClusterKit



TG HiSeq® 3000/4000 SR ClusterKit



Product Highlights

HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. Compatible with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Frequently Purchased Together

If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.


Supporting Data and Figures


Product Literature

Literature is not currently available for this product.

Manuals and Support Information

HiSeq 3000/4000 SBS Kit Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All HiSeq 3000/4000 SBS Kit  Support  

Best Practices for Storing Sequencing Reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

View Bulletin
Best Practices for Storing Sequencing Reagents

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