For use with the Genome Analyzer IIx with cBot, the TruSeq SR Cluster Kit v2-cBot-GA provides reagents that bind samples to complementary adapter oligos for cluster amplification on single-read flow cells.
The kit contains cluster generation reagents for the cBot cluster amplification system. The pre-mixed, 96-well plate format requires minimal reagent preparation.
DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high-fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.