Products
Products
Uses a rapid, single tube reaction to detect respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses) and antimicrobial resistance (1200+).
Uses a rapid, single tube reaction to detect respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses) and antimicrobial resistance (1200+).

Rapid Respiratory Pathogen ID/AMR Enrichment Panel Kit

Uses a rapid, single tube reaction to detect respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses) and antimicrobial resistance (1200+).

6.5 hr

Assay time

< 1.5 hr

Hands-on time

Based on volume (not concentration dependent)

Input quantity

See full details in the specifications table

Overview

The Rapid Respiratory Pathogen ID/AMR Enrichment Panel (Rapid RPIP) Kit is a next-generation sequencing (NGS)-based respiratory pathogen panel and library prep and enrichment kit that targets > 280 respiratory pathogens and > 2000 antimicrobial resistance (AMR) markers in a single assay, providing both pathogen identification and associated AMR information.

Key features

  • Comprehensive targeting of DNA and RNA respiratory pathogens and associated AMR markers/alleles that provide known predicted resistance to antimicrobial agents

  • Powerful pathogen quantitation using 10 commercially available spike-in controls

  • Robust and sensitive detection of low-abundance respiratory pathogen and AMR alleles using various sequencing read lengths and depths

  • Single-tube library prep reaction simplifies the workflow and reduces cleanup steps, improving detection of low-frequency targets

Streamlined, sample-to-results workflow

Together with DRAGEN Microbial Enrichment Plus, the flexible and scalable sample-to-results workflow delivers a rapid, cost-effective solution for identifying pathogens associated with respiratory tract infections in clinical research settings.

View the DRAGEN Microbial Enrichment Plus app

Specifications

Assay time 6.5 hr
Automation capability Liquid handling robot(s)
Automation details Explore available automation methods
Content specifications Detects respiratory pathogen DNA and RNA simultaneously and profiles antimicrobial resistance (AMR) gene expression concurrently.
Description Identify respiratory infections and co-infections, detect AMR markers, and perform strain typing of critical pathogens (SARS-CoV-2 and influenza A/B viruses) to study viral evolution and transmission.
Hands-on time < 1.5 hr
Input quantity Based on volume (not concentration dependent)
Instruments NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeq i100 Plus System, MiSeq i100 System
Mechanism of action On-bead tagmentation followed by a single hybridization step
Method Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Multiplexing Up to 384 samples in a single run with unique dual indexes
Nucleic acid type DNA, RNA
Species category Fungal, Virus, Bacteria
Technology Sequencing

Required Products

In addition to the Rapid Respiratory Pathogen ID/AMR Enrichment Panel Kit, you'll need a sequencing instrument, compatible sequencing reagents, and additional products listed in the user guide in the Documentation section.

/ Results

Applications

The Rapid Respiratory Pathogen ID/AMR Enrichment Panel Kit is used for the identification of > 280 respiratory pathogens (including Influenza and SARS-CoV-2) and > 2000 antimicrobial resistance markers in clinical research samples or general surveillance of respiratory pathogens/AMR in samples such as wastewater.

Example workflow

1
Prep

Rapid Respiratory Pathogen ID/AMR Enrichment Panel Kit

2
Sequence
3
Analyze

Project recommendations

Instrument Recommended number of samples Read length
MiSeq i100 System

1M paired-end reads for high concentration, low complexity samples; 4M paired-end reads for low concentration, high complexity samples, such as wastewater.

2 × 150 bp
NextSeq 550 System

1M paired-end reads for high concentration, low complexity samples; 4M paired-end reads for low concentration, high complexity samples, such as wastewater.

2 × 150 bp
NextSeq 1000 System, NextSeq 2000 System

1M paired-end reads for high concentration, low complexity samples; 4M paired-end reads for low concentration, high complexity samples, such as wastewater.

2 × 150 bp

Related applications and methods

Genomic surveillance of infectious diseases

NGS supports effective genomic surveillance strategies through identification of novel strains of coronavirus and other emerging infectious disease agents.

Documentation

Product literature
Support documentation

Compare

Rapid Respiratory Pathogen ID/AMR Enrichment Panel Kit Respiratory Pathogen ID/AMR Enrichment Panel Kit Rapid Respiratory Virus Enrichment Kit
Assay time 6.5 hr < 9 hr library prep time 6.5 hr
Automation capability Liquid handling robot(s) Liquid handling robot(s) Liquid handling robot(s)
Automation details Explore available automation methods Explore available automation methods
Content specifications Detects respiratory pathogen DNA and RNA simultaneously and profiles antimicrobial resistance (AMR) gene expression concurrently. Detects respiratory pathogen DNA and RNA simultaneously and profiles antimicrobial resistance (AMR) gene expression concurrently. Targets and characterizes ~40 common respiratory viruses, including SARS-CoV-2, influenza A and B viruses, adenovirus, rhinovirus, respiratory syncytial virus, and other common respiratory viruses.
Description Identify respiratory infections and co-infections, detect AMR markers, and perform strain typing of critical pathogens (SARS-CoV-2 and influenza A/B viruses) to study viral evolution and transmission. Identify respiratory infections and co-infections, detect antimicrobial resistance markers, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission. A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses. The agnostic design allows for widespread identification of pathogenic viruses across multiple sample types of interest
Hands-on time < 1.5 hr < 2 hr library prep time < 1.5 hr
Input quantity Based on volume (not concentration dependent) Based on volume (not concentration dependent) 10–100 ng
Instruments NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeq i100 Plus System, MiSeq i100 System MiSeq System, NextSeq 550 System, MiniSeq System, MiSeq i100 Plus System, MiSeq i100 System NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeq i100 Plus System, MiSeq i100 System
Mechanism of action On-bead tagmentation followed by a single hybridization step On-bead tagmentation followed by a single hybridization step On-bead tagmentation followed by a single hybridization step
Method Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Multiplexing Up to 384 samples in a single run with unique dual indexes Up to 384 samples in a single run with unique dual indexes Up to 384 samples per sequencing run
Nucleic acid type DNA, RNA DNA, RNA DNA, RNA
Species category Fungal, Virus, Bacteria Fungal, Virus, Bacteria Human, Virus
Technology Sequencing Sequencing Sequencing

Selection tools:

Library Prep & Array Kit Selector

Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.

Figures

figure-2-fast-library-prep-enrich-id-amr

Fast library prep and enrichment with reduced hands-on time

cDNA synthesis and on-bead tagmentation combined in a single reaction and a single, 90-minute hybridization deliver a fast workflow with reduced hands-on time compared to the standard protocol.

figure-3-detection-pathogens-id-amr

Sensitive detection and coverage of viral and bacterial pathogens

Microorganisms detected with the Rapid Respiratory Pathogen ID/AMR Panel are ordered by ascending coverage, including both viral and bacterial targets.

figure-7-detection-amr-markers-id-amr

Sensitive detection and coverage of antimicrobial resistance markers

AMR markers detected with the Rapid Respiratory Pathogen ID/AMR Panel are ordered by ascending coverage.

Enrichment panels (1)

Illumina Rapid Respiratory Pathogen ID/AMR (RPIP) Kit (96 samples)

20158828

Includes reagents for preparing and enriching 96 samples of Rapid RNA libraries (32 hybridization reactions with 3 libraries each), with the Respiratory Pathogen ID/AMR Panel, and Illumina Purification Beads. UD Indexes sold separately. Respiratory Pathogen ID/AMR Panel provides whole genome sequencing of > 280 respiratory pathogens and > 2000 antimicrobial resistance (AMR) markers.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Accessory products (4)

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Showing of

Selection summary

Product

Qty

Unit price

Total ( items)

You have not selected any items yet.

Please add these products to your cart before leaving the page to avoid losing them.

FAQ

The Rapid version uses a single tube reaction library prep that significantly shortens the hands-on time and assay time compared to the standard version. The oligo panel and the targets it detects are the same in both the standard and Rapid versions. The standard version includes a 96-sample index plate. The plate is sold separately for the Rapid version, allowing maximum flexibility in run planning.

Whole-genome information is provided for SARS-CoV-2 and Influenza A and B viruses only. Other targeted pathogens are identified based on small, highly informative targeted regions of each genome.

The panel in this kit includes probes that will recognize 10 different commercially available controls. Those controls, however, are not provided as part of this kit and must be purchased separately and then spiked into samples prior to DNA and RNA extraction. Only one spike-in control is necessary for each experiment/sample. Please contact your Illumina Sales Rep or Tech Support for further information.

We generally recommend starting with 1M single reads, or 2M paired-end reads (1M fragments) per sample using a minimum of 1 × 100 bp or 2 × 150 bp sequencing chemistries. These numbers are a starting point and should be optimized based on sample types and target abundance.

Yes. If your experiments can leverage the content and analysis provided by this kit and software, then you can use DNA and RNA extracted from a variety of sample types. Wastewater samples are often tested using this kit for surveillance of respiratory pathogens and their associated AMR. For wastewater samples, we generally recommend increasing the number of reads to 4M single reads or 8M paired-end reads.

/ Results

MiSeq i100 Series ordering

Simplified operations, advanced chemistry, and onboard informatics provide an easy-to-use, fast, and flexible instrument supporting a broad range of applications.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

TruSight Hereditary Cancer Panel

Targeted sequencing panel that assesses germline mutations across 113 genes and 125 SNPs associated with genetic cancer risk predisposition.

Illumina UD Indexes

Unique dual (UD) indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput and cost-saving benefits.

Illumina RNA Prep with Enrichment

A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.