HiSeq v4 reagents leverage the latest innovations in Illumina sequencing technology, offering improved cluster density and throughput, while enhancing the overall utility of the HiSeq platform. The kits contain ready-to-load reagents specifically designed for enabled* HiSeq sequencing systems.
The increased capacity and rapid turnaround time of the new v4 reagent kits enable researchers, in a single run, to process up to:
(Assumes 100 gigabases (Gb) per genome at 2x125bp, 5 Gb per exome at 2x100bp, 50 million reads per transcriptome)
Increase daily throughput
The HiSeq v4 reagent kits generate up to 1 terabase (1Tb) of data per 6-day run (up to 500 Gb per flow cell), increasing daily throughput to 167 Gb per day. The new v4 reagents increase the number of clusters by 33% compared to the TruSeq SBS Kit v3, adding additional capacity for counting assays.
|Kit Name||Output Max
(Per 2 Flow Cell)
|No. of Reads||Max Read Length||Time|
|HiSeq SBS V4 Kits||Up to 1 Tb||Up to 4 billion||2 x 125 bp||6 days|
HiSeq v4 Reagent kits
Flexible run configuration with a broad range of sequencing outputs
HiSeq systems continue to be the most flexible high-throughput sequencing systems on the market. The new v4 kits further improve output of the HiSeq system. You can choose from single or paired-end read runs, with read lengths up to 2×125 bp in high output mode. Enhanced rapid run kits now provide longer read lengths (up to 2×250 bp). These improvements continue to enable the broadest range of supported applications on the market, from ChIP-Seq, to whole-genome sequencing, to splice variant detection and more.
*Compatible with factory-built HiSeq 2500 and 1500 units and select HiSeq 2000 units with required upgrade. Please contact Customer Service to confirm system compatibility.