ScriptSeq Complete Gold (Blood) libraries were produced from 100 ng and 5 μg samples of DNA-free blood RNA. The coverage plots of the COX 5B transcript are consistent.
Profiles of RNA-Seq libraries made using the ScriptSeq Complete Gold Kit (Blood). ScriptSeq Complete Gold (Blood) libraries were prepared from 5 µg of RNA isolated from human whole blood (A) or cultured erythroid cells (B) and sequenced on an Illumina GAII. Fewer than 2% of the sequencing reads mapped to Globin RNA + rRNA.
Cluster-ready ScriptSeq RNA-Seq libraries, virtually free of globin mRNA and rRNA, are made in 1 day from as little as 100 ng of total blood RNA.
This table lists globin mRNAs and rRNAs removed by the Globin-Zero Reagents component of the ScriptSeq Complete Gold Kits (Blood).
|Adult Globin mRNAs Removed||Cytoplasmic (Nuclear-Encoded) rRNAs Removed||Mitochondrial rRNA Removed|
The low input versions of this kit (catalog numbers SCL6GBL and SCL24GBL) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.