ScriptSeq Complete Gold Kit (Blood)

This kit removes unwanted globin mRNA and ribosomal RNA from blood samples and generates sequencing libraries. Read More...
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ScriptSeq Complete Gold Kit (Blood) 6 reactions

BGGB1306

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ScriptSeq Complete Gold Kit (Blood) 24 reactions

BGGB1324

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Accessory Products
What accessories do I need?

ScriptSeq Index PCR Primers (Set 1)

RSBC10948

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ScriptSeq Index PCR Primers (Set 2)

SSIP1202

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ScriptSeq Index PCR Primers (Set 3)

SSIP1203

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ScriptSeq Index PCR Primers (Set 4)

SSIP1204

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ScriptSeq Index PCR Primers (Sets 1-4)

SSIP1234

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Product Highlights

  • Removes globin mRNA and ribosomal RNA (rRNA) in a single pass
  • Creates a stranded Illumina sequencing library
  • Sequencing data contains complete transcriptome of coding and non-coding RNA species
  • Good for intact and partially degraded samples
  • Suitable for all phases of research
  • Barcode/index-compatible: ScriptSeq index primers available separately for preparing barcoded libraries

Blood is an important sample for research into thousands of rare diseases and other disorders. The data from samples treated with ScriptSeq Complete Gold (Blood) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms for disease and health research.

ScriptSeq Complete (Blood) offers the most informative sequencing results by removing unwanted globin mRNA and rRNA prior to sequencing. When performing stranded analysis on samples prepared with ScriptSeq Complete Gold (Blood), choose the FR=secondstrand option.

Related Information

Specifications

Supporting Data and Figures

 

Globin mRNA and rRNA Types Removed

This table lists globin mRNAs and rRNAs removed by the Globin-Zero Reagents component of the ScriptSeq Complete Gold Kits (Blood).

Globin mRNAs and rRNAs removed by the Globin-Zero Reagents component
Adult Globin mRNAs Removed Cytoplasmic (Nuclear-Encoded) rRNAs Removed Mitochondrial rRNA Removed
Hba-a1
Hba-a2
Hbb
Hbg1 (human)
Hbg2 (human)
Hbb-b1 (mouse)
Hbb-b2 (mouse)
28S
5.8S
18S
5S
16S
12S

The low input versions of this kit (catalog numbers SCL6GBL and SCL24GBL) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.