AmpliSeq for Illumina Custom RNA Panel

Targeted, custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.

5.5-7.5 hours

Assay time

< 1.5 hr

Hands-on time

1 ng

Input quantity

See full details in the specifications table

Overview

Use AmpliSeq for Illumina Custom RNA Panels to measure gene expression in 12 to 1200 gene targets in a single assay. You can easily design custom targeted panels from a menu of over 20,000 human RefSeq genes using DesignStudio Software, a web-based assay design software tool.

Key features

  • Fast Gene Expression Profiling - Prepare libraries in 6 hours with < 1.5 hours hands-on time; results available in < 2 days.

  • Low-Quality, Low-Quantity Sample Input - Obtain high-quality data even when starting with minimal input from formalin-fixed, paraffin-embedded (FFPE) tissues.

  • DNA-to-Analysis Solution - Take advantage of a comprehensive workflow that spans library preparation, sequencing, and analysis.

An AmpliSeq for Illumina Custom RNA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.

Learn More About Ampliseq for Illumina


Specifications


Required products

The AmpliSeq for Illumina library preparation workflow requires a panel, library prep reagents (Library Plus), and indexes. Accessory products are also available for cDNA synthesis and sample identification.

AmpliSeq cDNA Synthesis for Illumina is required to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels.

AmpliSeq Library Equalizer for Illumina is an optional but recommended easy-to-use solution for normalizing libraries while using AmpliSeq for Illumina library prep methods.

AmpliSeq ERCC RNA Spike-In Mix for Illumina is a set of external RNA controls developed by the External RNA Controls Consortium (ERCC). The controls consist of a set of unlabeled, polyadenylated transcripts that can be used with AmpliSeq for Illumina assays with transcriptome panels to quantitate differential gene expression. Requires AmpliSeq ERCC RNA Companion Panel for Illumina (sold separately by Illumina).

AmpliSeq ERCC RNA Companion Panel for Illumina is intended for use with the AmpliSeq ERCC RNA Spike-In Mix for Illumina (sold separately by Illumina) to quantitate differential gene expression when performing AmpliSeq for Illumina assays with transcriptome panels.

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Applications

Example workflow


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High Concordance between Replicates

Libraries were prepared from 10 ng of Universal Human RNA using an AmpliSeq for Illumina Custom RNA Panel (208 amplicon panel size) and sequenced on the MiSeq System. Results show a high concordance (R2 = 0.997) between the two replicates of the same RNA sample. R2 is a statistical measurement of data correlation.

High Read Alignment

The AmpliSeq for Illumina Custom RNA Panel enables researches to analyze differential gene expression across genes of interest. To demonstrate assay performance, Human Brain Reference RNA and Universal Human RNA were analyzed using 5 AmpliSeq for Illumina Custom RNA panels of varying size (number of amplicons). Libraries were sequenced on a MiSeq System.

Panel (1)

AmpliSeq™ for Illumina® Custom RNA Panel

20020496

Targeted custom RNA panel. Library PLUS and index adapters sold separately.

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Library prep (3)

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.

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Index adapters (6)

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.

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Services (1)

AmpliSeq for Illumina RNA Library Prep Training

20023393

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Accessory products (2)

Ampliseq™ cDNA Synthesis for Illumina®

20022654

Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).

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AmpliSeq™ Library Equalizer for Illumina®

20019171

Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.

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Selection summary

Product

Qty

Unit Price

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Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

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Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

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