Support a wide range of NGS methods, including WGS, RNA-seq, exome/enrichment, amplicon, 16S metagenomics, and more
DRAGEN secondary analysis provides applications and pipelines for a wide range of genomic analysis capabilities. DRAGEN apps offer accurate, efficient, and secure analysis in the cloud on BaseSpace Sequence Hub. DRAGEN pipelines are data analysis tools that can be launched via an on-premises server, in the cloud on Illumina Connected Analytics, or onboard the NextSeq 1000 and NextSeq 2000 Systems and NovaSeq X Series.
| Application | DRAGEN On-Premises Server | NovaSeq X Series | NextSeq 1000/2000 | BaseSpace Sequence Hub | Illumina Connected Analytics |
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| BCL Convert | checkmark
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| DRAGEN ORA Compression | checkmark
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| DRAGEN FASTQ + MultiQC | checkmark
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| Whole genome | Germline + somatic | Germline + somatic (tumor only) | Germline only | Germline + somatic | Germline + somatic |
| Enrichment (including exome) | Germline + somatic | Germline + somatic | Germline + somatic | Germline + somatic | Germline + somatic |
| DRAGEN Amplicon | checkmark
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| RNA | checkmark
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| Single-Cell RNA | checkmark
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| NanoString GeoMx NGS (spatial transcriptomics) | Coming soon in v4.2 | checkmark
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| Methylation | checkmark
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| Metagenomics | checkmark
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| COVID | COVIDSeq, COVID lineage | COVIDSeq (cloud only) | COVIDSeq, COVID lineage | ||
| TruSight Oncology 500 | checkmark
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| ScATAC-Seq (single-cell sequencing assay for transposase-accessible chromatin) | checkmark
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| Imputation | checkmark
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| PGx Star Allele Caller | checkmark
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| Illumina Complete Long Reads | checkmark
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| RPIP and UPIP panels | Beta | checkmark
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Beta |
| Pipeline | Description | Variant types detecteda | Metrics provided | Publications |
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| DRAGEN Demultiplexing | Performs rapid demultiplexing of NGS analysis | |||
| DRAGEN ORA Compression | Optimized for high compression ratios of FASTQ files, as well as rapid compression and decompression, while preserving data integrity |
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Design considerations and methodology | |
| DRAGEN Map + Align | Can be run as a standalone or as part of the |
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Identification of SARS-CoV-2 variants
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| DRAGEN Germline | Provides NGS analysis, including advanced error model calibration for increased accuracy and repeat expansion detection and genotyping through Illumina Expansion Hunter | SNV Indel CNV SV Repeat expansions |
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| DRAGEN Somatic | Includes tumor-only and tumor–normal modes designed for detecting somatic variants in tumor samples. Both modes make no ploidy assumptions, enabling detection of low-frequency alleles | SNV Indel CNV SV TMB MSI HLA |
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Mutational landscape in multiple myeloma
Clonal evolution in multiple myeloma
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| DRAGEN Enrichment | Combines DRAGEN germline and somatic callers into a pipeline designed specifically for analyzing enrichment samples. Includes a full suite of enrichment metrics and reporting | SNV Indel CNV SV |
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| DRAGEN RNA | Performs transcriptome analysis starting with splice junction discovery and alignment, followed by rapid alignment and splice junction mapping and quantification. (For differential expression, Illumina recommends the DRAGEN Differential Expression app on BaseSpace Sequence Hub). | SNV Indel Gene fusion |
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Identifying point mutations in Bangladeshi SARS-CoV-2 strains |
| DRAGEN Single Cell RNA | Performs demultiplexing, cell-barcode and UMI error correction, sequence alignment, and quantification of gene expression |
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| DRAGEN Joint Genotyping | Calls variants jointly across multiple genomes and scales to large cohorts of samples at expedited speeds with uncompromising accuracy | SNV Indel CNV SV Repeat expansions |
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A hybrid cloud system for large-scale human genomic research |
| DRAGEN Methylation | Performs alignment and methyl calling. Calculates alignment and methylation metrics. |
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| DRAGEN Reference Builder | Accepts FASTA files and builds the proprietary reference used by the DRAGEN apps | |||
| DRAGEN TruSight Oncology 500 ctDNA Analysis Software | Offers secondary analysis support for TruSight Oncology 500 ctDNA. Only available on the local DRAGEN Server (version 3) | SNV Indel CNV DNA fusions MSI TMB |
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Development of blood TMB reference materials for validating cfDNA assays |
| DRAGEN Imputation | Enables scalable low-pass whole-genome sequencing analysis in a user-friendly tool | SNV Indel |
Boosting variant calling performance |
a. SNV, single nucleotide variant; CNV, copy number variation; SV, small variant; TMB, tumor mutational burden; MSI, microsatellite instability; HLA, human leukocyte antigen
Detect mutations and identify new variants, study COVID-19 host response, and contribute your findings to shared databases.
Combining Illumina DNA Prep with Enrichment for exome sequencing with the analytical power of DRAGEN enables efficient and accurate variant analysis in FFPE tumor samples.
Combining Illumina DNA Prep with Enrichment for exome sequencing with the analytical power of DRAGEN enables efficient and accurate variant analysis in FFPE tumor samples.
The NanoString GeoMx DSP combined with proven Illumina sequencing provides high-plexity spatial analysis and information on tissue architecture needed for gene and protein profiling.
View more details about DRAGEN analysis features, learn how to order, and find product documentation. You can also speak to a specialist to get your questions answered.
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