Nextera DNA Indexes

Nextera DNA Indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput.

Overview

Nextera DNA Indexes enable multiplexing of high-quality sequencing libraries generated from the Nextera XT DNA Library Preparation Kit or Illumina DNA Prep. Available in two adapter kits, the Nextera XT Index Kit v2 and Nextera DNA CD Indexes suit a range of throughput next-generation sequencing (NGS) applications. 

  • Provide high-throughput sequencing of up to 384 samples

  • Accommodate lower throughput sequencing application needs

  • Offer low-cost indexes for labs on a budget

Combinatorial dual indexes

Nextera DNA Indexes are combinatorial dual (CD) indexes and cannot eliminate index hopping artifacts during demultiplexing. CD indexes work best for applications that do not require high sensitivity and for multiplexing on sequencing systems that use nonpatterned flow cells, such as the MiniSeq, MiSeq, and NextSeq 550 Systems. 

It is recommended to use Illumina DNA/RNA UD Indexes for multiplexing on sequencing platforms with patterned flow cells such as the NextSeq 2000 System, NovaSeq 6000 System, and NovaSeq X Series to reduce index hopping or read misassignment during demultiplexing. Nextera XT v2 indexes and Nextera Combinatorial Dual (CD) indexes cannot eliminate read misassignment due to index hopping artifacts.

Additional information

Find kits compatible with Nextera DNA indexes and learn more about how to minimize index hopping on your next sequencing run. 


Specifications


Required products

The Nextera XT Index Kit v2 Sets A–D and Nextera XT Index Kit are compatible with the Nextera XT DNA Library Preparation Kit. The library preparation kit needs to be ordered separately. 

The Nextera DNA CD indexes are compatible with Illumina DNA Prep. The library preparation kit needs to be ordered separately.

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Index adapters (6)

Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.

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Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.

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Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.

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Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

Includes 96, 8 bp indexes sufficient for labeling 384 samples. Purchase library prep kit and purification beads separately.

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Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

Includes 24, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and purification beads separately.

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Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)

20018708

Includes 20 indexes, eight of i500s and 12 of i700 indexes that together provide 96 dual indexing capability sufficient for labeling 96 samples. Indexes are provided in a 96 well format. Purchase library prep and probe panels separately.

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FAQs

The  Illumina Adapter Sequences Document  lists index adapter sequences and all other adapter sequences.

Visiit our multiplex sequencing page for more information.

/ Results

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Illumina UD Indexes

Unique dual (UD) indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput and cost-saving benefits.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.