Sample multiplexing, also known as multiplex sequencing, allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on Illumina instruments. Sample multiplexing is useful when targeting specific genomic regions or working with smaller genomes. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.
With multiplex sequencing, individual "barcode" sequences are added to each DNA fragment during next-generation sequencing (NGS) library preparation so that each read can be identified and sorted before the final data analysis. These barcodes, or index adapters, can follow one of two major indexing strategies depending on your library prep kit and application.
Researchers leverage the speed and multiplex sequencing capabilities of Illumina sequencers to identify microbes along the Mississippi River.Read Interview
When preparing libraries for multiplexing, we recommend using unique dual indexes. Unique dual indexes allow you to increase the number of samples sequenced per run and reduce per-sample cost compared to other indexing strategies.Learn More
Unique molecular identifiers (UMIs) are a type of molecular barcoding that provides error correction and increased accuracy during sequencing. Sequencing with UMIs can reduce the rate of false-positive variant calls and increase sensitivity of variant detection.Learn More
With multiplexing, the potential for index hopping is present regardless of the library prep method or sequencing system used. Learn best practices to help minimize the effects of index hopping.Learn More
Researchers at the Chan Zuckerberg Biohub are working to conduct innovative experiments and form new collaborations in genomics. The NovaSeq System enables them to bundle many samples together for medical research that may help to improve human health.View Video
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Illumina sequencing allows researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism.
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