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TruSeq Custom Amplicon (TSCA) human library (New dataset - 2 x 300 bp with dual indexing)

A human TSCA library consisting of 24 replicates of NA18507 was prepared with a 425 bp 1536-amplicon panel consisting of SNP and exon targets. The library was sequenced on the MiSeq System using the new MiSeq Reagent Kit v3, at a 2 x 300 bp read length configuration with dual indexing. The total yield was 15.7 Gb with 80% of bases at or above Q30.

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View Project  » (alignment and variant calling, analysis with App Store, file downloads)

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E. coli MG1655 prepared with TruSeq Nano library prep kit (New dataset - 2 x 300 bp)

 E. coli strain MG1655 was sequenced on the MiSeq System using the new MiSeq Reagent Kit v3 and a read length configuration of 2 x 300 bp. The sequence data analysis showed 83% of bases above Q30.

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B. cereus sample prepared with TruSeq Nano library prep kit (New dataset - 2 x 300 bp)

A single B. cereus ATCC 10987 sample was sequenced on the MiSeq System using the new MiSeq Reagent Kit v3 at a 2 x 300 bp read length configuration. The total yield was 14.0 Gb with 85% of bases at or above Q30. The average fragment length for this data set is 550 bp.

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View Project  » (alignment and variant calling, analysis with App Store, file downloads)

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B. cereus and R. sphaeroides sample prepared with Nextera XT library prep kit (New dataset - 2 x 300 bp with dual indexing)

The B. cereus ATCC 10987 & R. sphaeroides ATCC BAA-808 samples were sequenced on the MiSeq System using the new MiSeq Reagent Kit v3 at a 2 x 300 bp read length configuration with dual indexing. The total yield was 17.8 Gb with 70% of bases at or above Q30. The average fragment length for this data set is 475 bp.

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View Project  » (alignment and variant calling, analysis with App Store, file downloads)

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PhiX (New dataset - 2 x 300 bp)

The PhiX bacteriophage genome was sequenced on the MiSeq System using the new MiSeq Reagent Kit v3 at a 2 x 300 bp read length configuration with TruSeq sample preparation kits. The total yield was 18.8 Gb with 88.8% of bases at or above Q30.

Browse the data in BaseSpace:

View Project  » (alignment and variant calling, analysis with App Store, file downloads)

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