Study gene expression using RNA sequencing

A high-resolution view of coding and noncoding regions of the transcriptome for a deeper understanding of biology

RNA Sequencing

RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing visibility to previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions and across a broad range of other study designs.

RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, allele-specific gene expression and other features without the limitation of prior knowledge.

RNA-Seq is increasingly the method of choice for researchers studying the transcriptome. It offers numerous advantages over gene expression arrays.

  • Broader dynamic range enables more sensitive and accurate measurement of gene expression
  • Not limited by prior knowledge - captures both known and novel features
  • Can be applied to any species, even if reference sequencing is not available
  • A better value, often delivering advantages at a comparable or lower price per sample than many arrays
Perspectives on RNA-Seq: Deeper Insights
Perspectives on RNA-Seq: Deeper Insights

RNA sequencing provides deeper insights for complex research. See how RNA-Seq is helping this lab move beyond gene expression.

Buyer’s Guide: Simple RNA Sequencing Workflows

Evaluating options for next generation RNA sequencing.

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New To Next Generation Sequencing?

Find out how Illumina NGS technology works and what types of experiments you can perform with next-generation sequencing (NGS).

About NGS Technology 
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How Can I Use NGS to Analyze RNA?

Learn about 7 key RNA-Seq methods. Find out how they differ to help you determine the method most appropriate for your research.

Show Key RNA-Seq Methods 
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How Can I Apply RNA-Seq?

Monitor gene expression and transcriptome changes with cancer RNA-Seq.

Understand host-pathogen immune interactions and more with microbial RNA-Seq.

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Transitioning to an RNA-Seq Workflow Solution
RNA-Seq Techniques in Action

See how easy it is to make the transition to next-generation RNA sequencing.

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Detecting changes in the cancer transcriptome
Detecting changes in the cancer transcriptome

Learn how RNA-Seq can aid in understanding tumor classification and progression.

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FAQs: RNA-Seq Data Analysis
FAQs: RNA-Seq Data Analysis

Demystifying data analysis with answers to some of the most common frequently asked questions.

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RNA-Seq Data Analysis
Easily Analyze RNA-Seq Data

User-friendly BaseSpace Apps simplify data analysis for the most common RNA sequencing study designs.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for common issues including overclustering, inconsistent quantitation, and sequencing through the insert.

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TruSeq RNA Access Kit
Keys to RNA Exome Sequencing

Tools for gene expression profiling and gene fusion detection in limited and degraded RNA samples.

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