Detecting transcriptome changes with RNA sequencing

A high-resolution view of coding and noncoding RNA activity for a deeper understanding of biology

RNA Sequencing

RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing visibility to previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions and across a broad range of other study designs.

RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, allele-specific gene expression and other features without the limitation of prior knowledge.

Introduction to RNA Sequencing

RNA-Seq is increasingly the method of choice for researchers studying the transcriptome. It offers numerous advantages over gene expression arrays.

  • Broader dynamic range enables more sensitive and accurate measurement of gene expression
  • Not limited by prior knowledge - captures both known and novel features
  • Can be applied to any species, even if reference sequencing is not available
  • A better value, often delivering advantages at a comparable or lower price per sample than many arrays
Propelling Progress with RNA-Seq

RNA sequencing can have far-reaching effects on research and innovation, transforming our understanding of the world around us.

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mRNA Sequencing

Accurately measure gene and transcript abundance and detect both known and novel features in the coding transcriptome.

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Total RNA Sequencing

Accurately measure gene and transcript abundance and detect both known and novel features in coding and multiple forms of noncoding RNA.

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Targeted RNA Sequencing

Measure the expression of transcripts or pathways of interest. Perform differential expression analysis, measurement of allele-specific expression, and detection of gene fusions.

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Small RNA Sequencing

Isolate and sequence small RNA species, such as microRNA, to understand the role of noncoding RNA in gene silencing and posttranscriptional regulation of gene expression.

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Ribosome Profiling

Deeply sequence ribosome-protected mRNA fragments to gain a complete view of all the ribosomes active in a cell at a specific time point and predict protein abundance.

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Ultra-Low-Input and Single-Cell RNA-Seq

Use deep RNA-Seq to examine the signals and behavior of a cell in the context of its surrounding environment. This method is advantageous for biologists studying cell function in time-dependent processes such as differentiation, proliferation, and tumorigenesis.

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RNA Exome Capture Sequencing

Detect both known and novel features of the RNA exome using sequence-specific capture of RNA coding regions. Achieve high-quality data from degraded samples.

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Find the Right RNA-Seq Library Prep Kit

Determine the best kit for your needs based on your sample type and method of interest. Access detailed product information, including comparison tables and specifications.

Find the Right Kit Find the Right Kit
Kit Selector

NGS offers an unlimited dynamic range and tunable resolution for gene expression profiling plus a wide variety of other applications. Discover the broad range of experiments you can perform with NGS, and find out how Illumina NGS technology works.

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Desktop RNA Sequencer

The NextSeq Series of sequencing systems offers an accessible, cost-effective RNA-Seq solution.

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Cancer Research with RNA-Seq
Cancer Research with RNA-Seq

Cancers accumulate numerous genetic changes, but typically only a few drive tumor progression. Monitoring gene expression and transcriptome changes with RNA-Seq can aid in understanding which variants are expressed, affecting tumor classification and progression. Learn about cancer RNA-Seq.

Microbial RNA-Seq
Microbial RNA-Seq

Microbial transcriptome and metatranscriptome information is important for predicting antibiotic resistance, understanding host–pathogen immune interactions, and tracking disease progression. RNA-Seq of bacterial and viral transcriptomes is a valuable method for analyzing this information. Explore microbial RNA-Seq.

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Perspectives on RNA-Seq: Novel Breakthroughs
Perspectives on RNA-Seq: Novel Breakthroughs

By taking a closer look at how viruses cause disease, Dr. Katze unravels the complex nature of RNA viruses.

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Perspectives on RNA-Seq: Deeper Insights
Perspectives on RNA-Seq: Deeper Insights

RNA sequencing provides deeper insights for complex research. See how RNA-Seq is helping this lab move beyond gene expression.

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Transitioning to an RNA-Seq Workflow Solution
RNA-Seq Techniques in Action

See how easy it is to make the transition to next-generation RNA sequencing.

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Simple RNA-Seq Workflows
Buyer’s Guide: Simple RNA Sequencing Workflows

Evaluating options for next-generation RNA sequencing.

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FAQs: RNA-Seq Data Analysis
FAQs: RNA-Seq Data Analysis

Demystifying data analysis with answers to some of the most common frequently asked questions.

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RNA-Seq Data Analysis
Easily Analyze RNA-Seq Data

User-friendly BaseSpace Apps simplify data analysis for the most common RNA sequencing study designs.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for common issues including overclustering, inconsistent quantitation, and sequencing through the insert.

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TruSeq RNA Access Kit
Keys to RNA Exome Sequencing

Tools for gene expression profiling and gene fusion detection in limited and degraded RNA samples.

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