Illumina Sequencing Methods

Accurate total NGS solutions including library preparation, sequencing systems, and data analysis


Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used.

Scientist with HiSeq
DNA Sequencing

Discover the entire genome with whole-genome solutions, or focus on specific regions of interest with whole-exome and targeted sequencing options.

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RNA Sequencing

Take advantage of a broad range of sequencing techniques for targeted RNA or whole-transcriptome studies.

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Methylation Sequencing

Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.

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Sequencing Method Explorer

Use the beta version of this interactive tool to explore experimental next-generation sequencing (NGS) library preparation methods compiled from the scientific literature. New methods will be continuously added.

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Method Selector

Library preparation kits are available for all sequencing methods, including whole-genome sequencing, whole-exome and targeted sequencing, RNA sequencing, methylation sequencing, and more. Kits accommodate a range of throughput needs, from manual protocols to fully automated workstations.

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Sequencing Library Preparation
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Compare Sequencing Platforms
Compare Sequencing Platforms

A variety of NGS platforms that deliver industry-leading data quality and accuracy. Find the one that’s right for you.

Compare NGS Platforms
NGS Introduction
Introduction to NGS

With its unprecedented throughput, scalability, and speed, NGS enables biological studies at a level never before possible.

Explore NGS
NextSeq 500
Improve Your Sequencing Results

NextSeq 500 v2 Kits offer better data quality, an improved signal to noise ratio, and a simpler workflow.

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Library Prep and Array Kit Selector
Library Prep and Array Kit Selector

Determine the best kit for your needs based on your project type, starting material, and method of interest.

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Illumina NGS Application Guide
Illumina NGS Methods Guide

All the information you need, from library preparation to sequencer selection to analysis. Select the best tools for your lab.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for common issues including overclustering, inconsistent quantitation, and sequencing through the insert.

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