Targeted cancer sequencing focuses on a select set of genes, gene regions, or amplicons that have known associations with cancer. You can use fixed panels that contain cancer-associated genes or design custom panels based on genes of interest.
Next-generation sequencing (NGS) enables massively parallel sequencing of multiple genes and samples at one time. This high-throughput method offers distinct advantages over traditional capillary electrophoresis, including increased scalability, speed, and resolution.1,2 By performing targeted cancer sequencing with a focused gene panel, you can reduce costs and complete studies more quickly.
Fixed panels offer the reassurance of expertly designed and analytically validated genomic content, focusing on genes with established cancer associations. This enables the highest sensitivity for rare variant calling in all sample types, including formalin-fixed, paraffin-embedded (FFPE) tissue.
With custom panels for targeted cancer sequencing, researchers can sequence hundreds of genomic regions of interest in a single panel. Gene content can be completely customized based on study objectives. Custom assays are compatible with various sample types, including FFPE tumors.
Focused panels for targeted cancer sequencing significantly narrow the scope of a sequencing project, reducing cost and data analysis burdens. Because it assesses a limited set of genes, targeted cancer sequencing allows for deeper coverage of those genes and higher sensitivity to call variants in rare tumor subclones confidently.
Illumina offers several library preparation, sequencing, and data analysis options for targeted cancer sequencing. Streamlined library prep workflows and flexible kit configurations accommodate multiple study designs. Illumina systems deliver industry-leading data quality—in fact, approximately 90% of the world’s sequencing data are generated using Illumina sequencing by synthesis (SBS) chemistry.
Push-button apps in BaseSpace simplify data analysis, so you can spend less time analyzing data and more time focusing on the next breakthrough.
Click on the below to view products for each workflow step.
An easy-to-use online software tool that provides dynamic feedback to optimize designs.TruSight Cancer Sequencing Panel
A fixed panel containing 94 genes and 284 SNPs associated with cancer predisposition.TruSight Tumor Sequencing Panel
A fixed panel targeting variations in solid tumors.TruSight Myeloid Sequencing Panel
A fixed panel targeting oncogenic exons and tumor suppressor genes for myeloid malignancies.TruSeq Amplicon - Cancer Panel
A fixed panel of somatic mutational hotspots in a broad spectrum of cancers.TruSeq Custom Amplicon
A fully customizable assay for targeted sequencing studies.Nextera Rapid Capture Custom Enrichment Kit
Customized probe design to target 0.5–25 Mb of custom content.
Speed and simplicity for desktop targeted cancer sequencing.MiSeq System
Desktop sequencer featuring a simple NGS workflow, integrated data analysis software, and unmatched accuracy.MiSeqDx Instrument
FDA-cleared in vitro diagnostic NGS system that also operates in research mode to generate accurate, reliable data.NextSeq Series
Flexible desktop sequencers that support multiple applications.
Easy-to-use software for analysis and variant calling on the MiSeq System.VariantStudio Software
A powerful analysis and reporting tool that provides biological insight into genomic variant data.BaseSpace Cloud or Onsite
The Illumina genomics computing environment for NGS data analysis and management.