Highly sensitive RNA sequencing (RNA-Seq) methods now enable gene expression analysis of very low-input samples and even single cells. Ultra-low-input and single-cell RNA-Seq offer higher resolution to discover cellular differences usually masked by bulk sampling methods.
Single-cell RNA-Seq lets you read the gene expression of one individual cell at a time. With these sensitive assays, you can explore the distinct biology of different cells within an organ or a tumor, and understand subpopulation responses to environmental cues.
The SMARTer® Ultra™ Low Input RNA Kit, sold and supported by Clontech, is a cDNA synthesis kit designed for single-cell or ultra-low-input RNA-Seq. The kit enables robust transcriptome analysis down to single-cell input levels in high-quality samples, and is optimized for Illumina sequencing.
The assay accurately synthesizes full-length cDNA from only 1-1000 whole cells or 10 pg-10 ng high-quality total RNA. The single-tube protocol works directly on whole cells and preserves sample integrity.
The Nextera XT DNA Library Preparation Kit is optimal for preparing an Illumina RNA sequencing library from cDNA generated with the Clontech SMARTer Ultra Low Input RNA kit.
Visit the RNA-Seq Data Analysis page to find user-friendly apps for analysis of RNA sequencing data.
For Research Use Only. Not for use in diagnostic procedures.
See selected publications using Illumina technology for single-cell analysis.
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Learn how researchers are using single-cell RNA-Seq to study: