MiSeq Reagent Kit v3

MiSeq Reagent Kits v3 offer enhanced chemistry to increase cluster density and read length, and improve sequencing quality scores. Read More...
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MiSeq Reagent Kit v3 (150-cycle)



MiSeq Reagent Kit v3 (600-cycle)


Product Highlights

MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. The v3 kits retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality (Q) scores.

MiSeq Reagent Kit v3 allows researchers to:

  • Double the output of a single run
  • Extend read lengths up to 2 x 300 bp with the 600-cycle kit
  • Increase the number of reads to 25 million to unlock new applications
Updated system software unlocks the full benefits of the v3 kits. Access the latest software.


The MiSeq v3 kits can achieve double the amount of output per flow cell. Kits are available in a 600-cycle format to allow the longest read lengths on any Illumina sequencing system. There is also a 150-cycle format that enables counting applications. In addition, all MiSeq Reagent Kit v2 configurations will continue to be available. MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

Reagent Kit No. of Reads Kit Size (cycles) Output (max.) 2 × 75 Output 2 × 300 Output
MiSeq Reagent Kit v3 25 M 150, 600 15 Gb 3.8 Gb 15 Gb

Gb = gigabases, M = millions


Product Literature

MiSeq Applications Brochure

Brochure | PDF(3 MB)

MiSeq System Specification Sheet

Data Sheet | PDF(< 1 MB)

Manuals and Support Information

MiSeq Reagent Kit Documentation  

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All MiSeq Reagent Kit Support