Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2500, HiSeq 2000, HiSeq 1000, HiScanSQ, or Genome Analyzer:
- Any Nextera library with any number of indices for single-read or paired-end runs. Use the single-read kit with a single-read flow cell, and the paired-end kit with a paired-end flow cell.
- TruSeq HT libraries when performing a dual-indexed run on a single-read flow cell.
- TruSeq Synthetic Long-Read DNA libraries when sequencing using the TruSeq SBS Kit v3 and performing cluster generation with the TruSeq PE Cluster Kit v3.
Each box includes sufficient reagents for processing a single flow cell.