HiSeq 2500/1500
Unprecedented Flexibility for Multiple Applications
The HiSeq 2500 system features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output modes offering scalable output to fit your project needs. The HiSeq 1500 system provides the same run mode functionality with support for a single flow cell rather than two.
Rapid run mode provides quick results.
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, which enable greater depth of coverage and improved assembly for de novo applications.
High output mode is perfect for larger studies.
High output mode is perfect for larger studies with more samples or when the greatest depth of coverage is required. High output mode allows you to batch process ~5× more samples than rapid mode and to complete large projects in record time.
Rapid Run Mode | High Output Mode |
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|---|---|---|---|
| ChIP-Seq Transcription Factor 15M Reads 1 x 36 bp |
40 Samples 7 Hours |
200 Samples 2 Days |
|
| mRNA-Seq >50M Reads 2 x 50 bp |
24 Samples 16 Hours |
120 Samples 5 Days |
|
| TruSeq Exome Seq 62 MB Region 100x Coverage 2 x 100 bp |
15 Samples 27 Hours |
85 Samples 11 Days |
|
| Human Whole Genome >30x Coverage 2 x 100 bp |
1 Sample 27 Hours |
5 Samples 11 Days |
|
| De novo Sequencing 1.5 GB Genome 100x Coverage 2 x 150 bp |
1 Sample 40 Hours |
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Values in this figure are examples based on system performance capabilities using two flow cells. Variation can be expected due to cluster density, sample quality and other experimental factors. Run times include on-board cluster generation and sequencing for rapid run mode and sequencing only for high output mode. Indexing run times are not included.
