QUESTIONS REQUEST PRICING

Cluster Generation and Sequencing

MiSeq Reagent Kit v2
Read LengthTotal Time*Output
1 × 36 bp
~4 hrs 540-610 Mb
2 × 25 bp
~5.5 hrs 750-850 Mb
2 × 150 bp
~24 hrs 4.5-5.1 Gb
2 × 250 bp ~39 hrs 7.5-8.5 Gb
MiSeq Reagent Kit v3
Read LengthTotal Time*Output
2 × 75 bp ~24 hrs
3.3-3.8 Gb
2 × 300 bp ~65 hrs
13.2-15 Gb

 

Reads Passing Filter†

MiSeq Reagent Kit v2
Single Reads 12-15 M
Paired-End Reads 24-30 M
MiSeq Reagent Kit v3
Single Reads 22-25 M
Paired-End Reads 44-50 M

 

Quality Scores††

MiSeq Reagent Kit v2
> 90% bases higher than Q30 at 1 x 36 bp
> 90% bases higher than Q30 at 2 x 25 bp
> 80% bases higher than Q30 at 2 x 150 bp
> 75% bases higher than Q30 at 2 x 250 bp
MiSeq Reagent Kit v3
> 85% bases higher than Q30 at 2 x 75 bp
> 70% bases higher than Q30 at 2 x 300 bp

*Total time includes cluster generation, sequencing, and basecalling on a MiSeq System enabled with dual-surface scanning.
†Install specifications based on Illumina PhiX control library at supported cluster densities (between 880-965 k/mm2 clusters passing filter for v2 chemistry and 1200-1400 k/mm2 raw clusters for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.
††A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
bp = base pairs, Mb = megabases, Gb = gigabases, M = millions.