NextSeq Series Specifications

NextSeq Series Sequencing System Performance Parameters

NextSeq Series High Output Kit *
Read Length Total Time† Output
2 × 150 bp ~29 hrs 100-120 Gb
2 × 75 bp 18 hrs 50-60 Gb
1 × 75 bp 11 hrs 25-30 Gb
NextSeq Series Mid Output Kit *
Read Length Total Time† Output
2 × 150 bp 26 hrs 32.5-39 Gb
2 × 75 bp 15 hrs 16.25-19.5 Gb

 

Reads Passing Filter

NextSeq Series High Output Kit
Single Reads Up to 400 Million
Paired-End Reads Up to 800 million
NextSeq Series Mid Output Kit
Single Reads Up to 130 Million
Paired-End Reads Up to 260 Million
 

 

Quality Scores††

NextSeq Series High Output Kit
> 75% bases higher than Q30 at 2 x 150 bp
> 80% bases higher than Q30 at 2 x 75 bp
> 80% bases higher than Q30 at 1 x 75 bp
NextSeq Series Mid Output Kit
> 75% bases higher than Q30 at 2 x 150 bp
> 80% bases higher than Q30 at 2 x 75 bp
 

 

NextSeq 550 System Array Scanning Parameters
BeadChip Scan Time Per BeadChip Scan Time Per Sample
Infinium CytoSNP-850K BeadChip 40 minutes 5 minutes
HumanCytoSNP-12 BeadChip 40 minutes 3.3 minutes
Infinium HumanKaryomap-12 BeadChip 40 minutes 3.3 minutes
 

 

View NextSeq System Series specification sheet (PDF) »


* Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 500 kits are paired-end capable.
† Total time includes cluster generation, sequencing, and base calling on a NextSeq 500 System enabled with dual-surface scanning.
†† A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.

 

For Research Use Only.