Find the Best System for Your Needs

  • MiniSeq System
  • MiSeq Series
  • NextSeq System
  • HiSeq Series
  • HiSeq X Series
 
MiniSeq System
MiSeq Series
NextSeq System
HiSeq System
HiSeq X System

MiSeq Series

Max Output

15 Gb

Max Read Number

25 million

Max Read Length

2×300 bp

Sequencing Platform Selector

Compare detailed sequencing platform specifications

Sequencing Platform Selector

This tool will help you compare next-generation sequencing systems and find the one that’s right for your lab or application.

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Research Use Only Molecular Diagnostics Forensic Genomics

Cancer Genomics Agrigenomics Genetic Disease Microbial Genomics Reproductive Health Population-scale WGS Other Area of Interest

Large Whole-Genome Sequencing (> 5 Mb) Small Whole-Genome Sequencing (≤ 5 Mb) Exome Sequencing or Large Gene Panels Amplicon Sequencing or Small Gene Panels Targeted RNA Sequencing Whole-Transcriptome Sequencing Small RNA Analysis ChIP-Seq Methylation Sequencing Metagenomics

MiSeqDx

Next-generation sequencing (NGS) technology can provide a broad view of the human genome, helping us discover and apply relevant genomic information to human health. Ultimately, this may lead to more accurate diagnosis, prognosis, and therapeutic selection. Clinical laboratories can take advantage of the benefits of NGS with the FDA-cleared MiSeqDx system.

At the heart of the system is the FDA-cleared MiSeqDx instrument, stringently validated for design and performance. Two IVD assays and one IVD kit are currently available for use on the MiSeqDx instrument:

  • The MiSeqDx Universal Kit allows users to develop their own NGS laboratory tests, expanding their lab offerings to meet ever-evolving needs
  • The MiSeqDx Cystic Fibrosis 139-Variant Assay detects 139 clinically relevant and functionally verified CFTR variants
  • The MiSeqDx Cystic Fibrosis Clinical Sequencing Assay detects mutations within the protein coding regions and intron/exon boundaries of CFTR

MiSeq FGx

The MiSeq FGx Forensic Genomics System is the first fully validated sequencing system designed for use in forensic genomics applications. The complete sample-to-answer system uses dedicated library preparation kits, customized instrument control software, and an analytical software package to support the reliable analysis of both routine and challenging forensic samples.

The MiSeq FGx System workflow is easy-to-use, automated, and fast:

  • Compact, all-in-one platform incorporates cluster generation, paired-end fluidics, and SBS chemistry
  • Intuitive touch screen interface makes for simple instrument operation
  • Plug-and-play reagents with RFID tracking make for added speed and convenience

HiSeq X Ten & HiSeq X Five

The HiSeq X Series delivers the first truly affordable genome—on a massive scale. Scientists, institutions, and nations are now empowered to create a comprehensive catalog of genetic variation, forge population-based references, drive far-reaching discoveries, and advance our understanding of biology and genetic disease.

The HiSeq X Ten and HiSeq X Five Systems take sequencing to the next level:

  • Designed and optimized for population- and production-scale whole-genome sequencing
  • Advanced patterned flow cell technology generates massive throughput
  • The $1000 genome is now a reality with the HiSeq X Ten System
  HiSeq X Series*
  HiSeq X Five HiSeq X Ten
Output Range 900–1800 Gb 900–1800 Gb
Run Time <3 days <3 days
Reads per Run 3–6 billion 3–6 billion
Maximum Read Length 2 × 150 bp 2 × 150 bp
Samples per Run 8–16 8–16
Relative Price per Sample Higher Cost Lower Cost
Relative Instrument Price Lower Cost Higher Cost
Downloads Spec Sheet Spec Sheet

* Specifications shown for an individual HiSeq X Sequencing System. HiSeq X is only available as part of the HiSeq X Five or HiSeq X Ten.

† For Research Use Only. Not for use in diagnostic procedures.

‡ Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.

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MiniSeq System
Low-throughput targeted DNA and RNA sequencing.
MiSeq Series
Small genome, amplicon, and targeted gene panel sequencing.
NextSeq System
Exome, transcriptome, and targeted resequencing.
HiSeq Series
Production-scale genome, exome, transcriptome sequencing, and more.
  MiniSeq System MiSeq Series NextSeq System HiSeq Series
  MiniSeq MiSeq* MiSeqDx MiSeq FGx NextSeq 500* HiSeq 2500* HiSeq 3000* HiSeq 4000*
Output Range 1.8–7.5 Gb 0.3–15 Gb 0.3–15 Gb 0.3–15 Gb 20–120 Gb 10–1000 Gb 125–750 Gb 125–1500 Gb
Run Time 4–24 hr 5–55 hr 4–55 hr 4–55 hr 11–29 hr 7 hr–6 days <1–3.5 days <1–3.5 days
Reads per Run 8–25 million 1–25 million 1–25 million 1–25 million (for research) 130–400 million 300 million–4 billion 2.5 billion 2.5–5 billion
Max Read Length 2 × 150 bp 2 × 300 bp 2 × 300 bp 2 × 300 bp 2 × 150 bp 2 × 250 bp (Rapid run)
2 × 125 bp (High output)
2 × 150 bp 2 × 150 bp
Samples per Run§ N/A N/A N/A N/A 1 1–8 6 6–12
Samples per Run§ 1–384 1–384 N/A 1–96 96 96–1536 768 768–1536
Samples per Run§ N/A N/A N/A N/A 12 150 90 180
Samples per Run§ 1–96 1–96 N/A N/A 12–36 36–228 N/A N/A
Samples per Run§ 1–384 1–384 N/A N/A 1–384 1–6144 1–6144 1–6144
Samples per Run§ N/A N/A N/A N/A 2–8 12–80 50 50–100
Samples per Run§ 1–5 1–5 N/A N/A 25–80 60–792 60–792 60–792
Samples per Run§ 1–6 1–6 N/A N/A 8–24 20–260 160 160–320
Samples per Run§ N/A N/A N/A N/A 1 10 2–3 4–6
Samples per Run§ 1–384 1–384 N/A N/A 1–384 N/A N/A N/A
Relative Price per Sample§ Higher Cost Higher Cost Higher Cost Higher Cost Mid Cost Lower Cost Lower Cost Lower Cost
Relative Instrument Price§ Lower Cost Lower Cost Lower Cost Lower Cost Mid Cost Higher Cost Higher Cost Higher Cost
Downloads Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet Spec Sheet

* For Research Use Only. Not for use in diagnostic procedures.

† For In Vitro Diagnostic applications.

‡ For Research, Forensic, and Paternity Use Only.

§ Based on 30X coverage of a human genome, assumes minimum of 100Gb per genome. Sample number will vary for other species based on relative genome size.

§ Based on 30X or greater coverage of a 5Mb microbial genome. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ Based on 4Gb per sample at 2 × 75 bp. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ Based on TruSight One Sequencing Panel. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 10 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 50 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 5 million reads per sample. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 15 million reads per sample for identification of transcription factor binding. Additional reads required for histone marks. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§ 30X coverage. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.

§Based on Nextera XT indexing. "Price per Sample" and "Instrument Price" relative to the other instruments shown in comparison results.



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Illumina Next-Generation Sequencing Platforms

Illumina offers innovative next-generation sequencing (NGS) platforms that deliver industry-leading data quality and accuracy, at an unparalleled scale. With flexible throughput and simple, streamlined workflows, our sequencing platforms are transforming the field of genomics.

Illumina technology empowers more and more NGS users every day. NGS has transformed the genomics community to include clinical, agrigenomics, and forensic genomics researchers, and continues to enable advances in cancer genomics, microbiology, and other areas.

NGS Platforms with Proven Performance

Illumina NGS platforms have been adopted by leading institutions around the world, both big and small, and are the production platform for all major genome centers. More bases have been sequenced using Illumina chemistry than all other next-generation sequencing platforms combined.

Our track record of relentless innovation is evident in the number and breadth of research publications. Illumina NGS platforms enable the broadest suite of sequencing applications, including the first FDA-cleared NGS system for diagnostic applications.

Our newly expanded sequencing platform portfolio will transform your research and enable new discoveries. Illumina systems deliver what you need, when you need it.

Each platform delivers industry-leading data quality and performance, with flexible throughput and simple, streamlined workflows—for every kind of researcher, for every scale of study.

Simple, Fully Integrated Sequencing Solutions

Rapid, high-quality NGS library preparation is a key element of the integrated sequencing platform workflows that Illumina offers. The easy-to-use library preparation and enrichment products enable sequencing of a wide variety of samples—including challenging, low-input, and even FFPE samples.

Illumina also offers a broad range of intuitive bioinformatics applications for data analysis, alignment, variant calling and more. Sequencing data can be instantly and securely transferred, stored, and analyzed in BaseSpace Sequence Hub, our genomics computing environment.

Results are presented in intuitive, easy-to-interpret reports, enabling rapid identification of variants, and the ability to instantly share data with collaborators around the world.

Illumina users can also access a rich ecosystem of third-party tools for data analysis.

Next-Generation Sequencing Platform Innovation is in our DNA

Illumina has pioneered major advances for NGS platforms in simplicity and flexibility – all while delivering industry-leading performance. Sequencing output has grown a thousand-fold, from less than 1 gigabase to over 1 terabase per run, while the number of reads has increased from millions to billions. Experiments that once required complex workflows now use simple push-button sequencing. Illumina continues to lead the field in sequencing platform innovation and performance.

Read the sequencing platform portfolio brochure (PDF) to learn more.