RNA-Seq technology allows you to discover and profile the entire transcriptome in any organism. With no probes or primers to design, RNA-Seq delivers unbiased and unparalleled information about the transcriptome. The simple sequencing workflow and Illumina's optimized TruSeq RNA Sample Prep technology allows you quickly process RNA samples to analyze novel transcripts, novel isoforms, alternative splice sites, rare transcripts, and cSNPs in one experiment.
Sequencing-based RNA analysis records the numerical frequency of a sequence in the library population, eliminating background signals observed using relative expression profiles generated with microarray hybridization technology. RNA-Seq offers a new level of assay customizability to optimize each experiment to a study’s specific design and purposes. Coverage depth can be tuned by simply sequencing more or less of the sample. This customizable depth enables a range of applications, from expression profiling and sample classification with low read count to rare transcript or variant discovery with deep coverage.