Paired-End Sequencing Assay

Paired-end sequencing allows users to choose the length of the insert and sequence either end of the insert, generating high-quality, alignable sequence data. Paired-end sequencing can detect rearrangements, including insertions and deletions (indels) and inversions. As paired end reads are more likely to align to a reference, the quality of the entire data set improves.

A simple modification to the standard single-read DNA library preparation facilitates reading both the forward and reverse template strands of each cluster during one paired-end read. Both reads contain long range positional information, allowing for highly precise alignment of reads.

Paired-End Sequencing Highlights

  • Simple Paired-End Libraries: Simple workflow allows generation of unique ranges of insert sizes
  • Efficient DNA Use: Requires the same amount of DNA as single-read, 50 ng–1 μg gDNA or cDNA
  • Broad Range of Applications: Does not require methylation of DNA or restriction digestion; can be used for bisulfite sequencing
  • Simplified Data Analysis: Higher quality sequence assemblies with short-insert libraries

Paired-End Sequencing and Alignment

Paired-end sequencing assay

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