Illumina sequencing technology, sequencing by synthesis (SBS), is the most successful and widely adopted next-generation sequencing (NGS) technology worldwide. Illumina sequencing instruments and reagents support massively parallel sequencing using a proprietary method that detects single bases as they are incorporated into growing DNA strands.
A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all 4 reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias.
The end result is true base-by-base sequencing that enables the industry's most accurate data for a broad range of applications. The method virtually eliminates errors and missed calls associated with strings of repeated nucleotides (homopolymers).
Diverse library preparation methods enable a variety of sequencing applications, such as whole-genome sequencing, de novo sequencing, candidate region targeted resequencing, DNA sequencing, RNA sequencing (for applications such as transcriptome and small RNA analysis), methylation analysis, and protein-nucleic acid interaction analysis (ChIP-Seq).
Illumina sequencing by synthesis technology supports both single-read and paired-end libraries. SBS technology is the only platform that offers a short-insert paired-end capability for high-resolution genome sequencing, as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, structural variation detection, and more.
The combination of short inserts and longer reads increases the ability to fully characterize any genome.
Find out how 2-channel SBS, a recent evolution of SBS technology, enables faster sequencing with the same high data accuracy.
Learn more about 2-channel SBS »
See SBS technology in action.
The NextSeq Series enables everyday genome, exome, transcriptome sequencing, and more.
View System »
NextSeq 500 v2 Kits offer better data quality, an improved signal to noise ratio, and a simpler workflow.
View Kits »
Read an in-depth introduction to Illumina sequencing technology.
Read Introduction (PDF) »
See how patterned flow cell technology dramatically increases sequencing data throughput.
Learn More »
Find highlights of recently published research using Illumina sequencing technology.
Read Reviews »