Bovine SNP50 Whole-Genome Genotyping Kits

Bovine SNP50 DNA Analysis Kits

System: iScan, BeadStation, BeadArray Reader, HiScanSQ
Technology: BeadArray
Assay: Infinium DNA Analysis Assay
Applications: Focused Genotyping
Species: Bovine
Contents: Each package contains 2 to 48 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 to 1152 samples

The BovineSNP50 v2 DNA Analysis BeadChip was developed by Illumina in collaboration with the USDA-ARS, University of Missouri, and the University of Alberta. The BeadChip contains 54,609 informative SNPs uniformly distributed across the entire genomes of major cattle breed types. All SNP probes on have been validated in 19 common beef and dairy breeds. This empowers applications such as genome-wide enabled selection, identification of quantitative trait loci, evaluation of genetic merit of individuals, and comparative genetic studies.

More than 24,000 SNP probes target novel SNP loci that were discovered by sequencing three pooled populations of economically important beef and dairy cattle using Illumina's Genome Analyzer. Additional content is derived from publicly available sources such as the bovine reference genome, Btau1, and the Bovine HapMap Consortium data set. This product targets evenly distributed SNPs that are polymorphic across the breeds tested and provides an average probe spacing of 49.4kb and a median spacing of 36.9kb.

The BeadChip is a multi-sample genotyping panel powered by Illumina's Infinium HD Assay. This assay provides the industry's highest call rates, allows for flexible content deployment, and enables the detection and measurement of copy number variation.

  • This 24-sample BeadChip represents the highest density genotyping solution for characterizing the genome in dairy and beef cattle
  • The PCR-free, single tube sample preparation significantly reduces labor and potential sample handling errors
  • Laboratory Information Management System (LIMS) and robotic automation are available to accurately and efficiently track samples throughout analysis

Order Information

WG-450-2001
Bovine
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Required Products

Specifications

BovineSNP50 v2 BeadChip 
Number of Markers 54,609
Samples per BeadChip 24
DNA Requirement 200ng
Assay Infinium HD
Instrument iScan or HiScanSQ
ParameterValue From
Reference Samples
Product
Specification
Call Rate
99.7%
> 99%
Reproducibility 100% > 99.9%
Mendelian Inconsistencies
0.06%
< 0.1%

 

 


Applications

Focused Genotyping

Focused genotyping supports a variety of applications such as candidate-gene studies in cancer, cardiovascular disease, and admixture mapping. Illumina also works closely with major animal consortia to develop genome-wide genotyping products for non-human organisms, including both animal and plant species.

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