Nextera DNA Library Preparation Kit

Nextera DNA kits provide the fastest, easiest library prep workflow, delivering whole-genome sequencing libraries in under 90 min. Read More...
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Nextera DNA Library Prep Kit (24 samples)



Nextera DNA Library Prep Kit (96 samples)


Accessory Products
What accessories do I need?

Nextera Index Kit (24 indexes, 96 samples)



Nextera Index Kit (96 indexes, 384 samples)


Product Highlights

Nextera DNA Library Preparation Kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatible with all Illumina sequencers.

The industry's fastest library prep

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.


Method-Specific Workflow Example


Customer Stories

Surveying the Chromatin Landscape with Next-Generation Sequencing

Researchers develop novel sequencing methods with the MiSeq and HiSeq Systems to understand the epigenome and its impact on cancer and immune diseases.

Learn More
Unmasking the Viral Etiology of Cancer and Immune Disease

Karolinska Institutet researchers use Illumina sequencers to identify novel HPV types associated with non-melanoma skin cancers.

Read More

Supporting Data and Figures


Product Literature

Nextera Low Plex Pooling Guidelines

Technical Note | PDF(< 1 MB)

Nextera DNA Library Preparation Kits

Data Sheet | PDF(< 1 MB)

Manuals and Support Information

Nextera DNA Library Prep Kit Support Documentation  

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All Nextera DNA Library Prep Kit Support 

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