Infinium HumanMethylation450 BeadChip Kit

The Infinium HumanMethylation450 BeadChip array offers a unique combination of comprehensive, expert-selected coverage, high sample throughput, and an affordable price, making it an ideal solution for epigenome-wide association studies (EWAS).

Powered by the revolutionary Illumina Infinium Methylation Assay, this BeadChip allows researchers to interrogate more than 485,000 methylation sites per sample at single-nucleotide resolution. Content was selected with the guidance of a consortium of methylation experts comprising 22 members that represent 19 institutions worldwide.  

The array covers 99% of RefSeq genes, with an average of 17 CpG sites per gene region distributed across the promoter, 5'UTR, first exon, gene body, and 3'UTR. It covers 96% of CpG islands, with additional coverage in island shores and the regions flanking them. Further content categories requested by the Consortium include:

  • CpG sites outside of CpG islands
  • Non-CpG methylated sites identified in human stem cells
  • Differentially methylated sites identified in tumor versus normal (multiple forms of cancer) and across several tissue types
  • CpG islands outside of coding regions
  • miRNA promoter regions

This BeadChip has a 12-sample per array format compatible with automation. This enables up to 96 samples to be run in parallel (with full LIMS support available), and requires only 500 ng of input (1 µg with automation). In addition, an FFPE-compatible protocol is now available.

This unique combination of features makes the HumanMethylation450 BeadChip a powerful tool to drive exciting new study designs and further fuel the rapid evolution of epigenetics research.

Related Links

Genotyping Studies with Illumina Omni BeadChips »

System Compatibility

iScan
HiScan

Product Literature

Description Document Type File Info
CpG Loci Identification Technical Note PDF (< 1 MB)
HumanMethylation450 BeadChip Data Analysis Technical Note Technical Note PDF (< 1 MB)
Infinium HumanMethylation450 BeadChip Product Information Sheet PDF (< 1 MB)
Human Epigenome-Wide Association Studies Application Note Application Note PDF (< 1 MB)
Infinium Methylation BeadChip Application Note Application Note PDF (< 1 MB)
HumanMethylation450 BeadChip Data Sheet Data Sheet PDF (< 1 MB)
Analyzing FFPE Samples with Infinium Microarrays Technical Note Technical Note PDF (< 1 MB)
Improved Staining Procedure for the Infinium Methylation Assay Technical Note PDF (< 1 MB)

Catalog IDs: WG-314-1003, WG-314-1001, WG-314-1002