MiSeq Reagent Kit v3

MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. MiSeq Reagent Kits v3 retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality (Q) scores. Updated system software unlocks the full benefits of v3 kits. Download Software »

MiSeq Reagent Kit v3 allows researchers to:

  • Double the output of a single run
  • Extend read lengths up to 2x300 with the 600-cycle kit
  • Increase the number of reads to 25 million to unlock new applications

The MiSeq v3 kits can achieve double the amount of output per flow cell. Kits are available in a 600-cycle format to allow the longest read lengths on any Illumina sequencing system. There is also a 150-cycle format that enables counting applications, and all v2 configurations will continue to be available. MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq system to validate compatibility with user-defined applications.


MiSeq Reagent Kit No. of Reads Kit Size (cycles) Output (max.) 2 × 75 Output 2 × 300 Output
MiSeq Reagent Kit v325 M150, 60015 Gb3.8 Gb15 Gb


Gb = gigabases, M = millions

System Compatibility

MiSeq System

Product Literature

Description Document Type File Info
MiSeq System Product Information Sheet Data Sheet PDF(< 1 MB)
MiSeq Applications Brochure Brochure PDF(3 MB)

Catalog IDs: MS-102-3001, MS-102-3003