TruSeq Custom Amplicon (TSCA) allows sequencing of hundreds of genomic regions covering up to 96 kb of cumulative sequence. This highly targeted approach enables a wide range of applications for discovering, validating, and screening genetic variants in a rapid and efficient manner.

Requiring just 250 ng of starting gDNA, TSCA is amenable to a wide range of sample types, even those available in limited quantity. With the ability to generate up to 384 amplicons per sample and integrated indexes to support up to 96 samples per MiSeq run, TSCA provides an unprecedented level of sample multiplexing.

TruSeq Custom Amplicon offers a fully integrated DNA-to-data solution, including convenient probe design and ordering using DesignStudio, an easy-to-use online software tool that provides dynamic feedback to optimize design and region coverage. Automated data analysis using the Illumina Amplicon Viewer software allows researchers to easily review project data, including variants detected, across multiple MiSeq runs.

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Learn more about TruSeq technology.

Webinars

Truseq Custom Amplicon: Learn how to customize content with DesignStudio

Workflow

The TruSeq Custom Amplicon Assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
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Catalog IDs: FC-130-1001, FC-130-1003, FC-130-1006, FC-130-1007