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MiSeq Reagent Kit v3

MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. MiSeq Reagent Kits v3 retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality (Q) scores. Updated system software unlocks the full benefits of v3 kits. Download Software »

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MiSeq Reagent Kit v3 allows researchers to:

  • Double the output of a single run
  • Extend read lengths up to 2x300 with the 600-cycle kit
  • Increase the number of reads to 25 million to unlock new applications

The MiSeq v3 kits can achieve double the amount of output per flow cell. Kits are available in a 600-cycle format to allow the longest read lengths on any Illumina sequencing system. There is also a 150-cycle format that enables counting applications, and all v2 configurations will continue to be available. MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq system to validate compatibility with user-defined applications.

 

MiSeq Reagent KitNo. of ReadsKit Size (cycles)Output (max.)2 × 75 Output2 × 300 Output
MiSeq Reagent Kit v3 25 M 150, 600 15 Gb 3.8 Gb 15 Gb

 

Gb = gigabases, M = millions


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MiSeq Reagent Kit v3
MS-102-3001
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TruSeq Stranded mRNA Sample Prep Kit

The TruSeq Stranded mRNA Sample Preparation kit provides the clearest and most complete view of the transcriptome with a streamlined, cost-efficient and scalable solution for mRNA analysis.

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Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

The TruSeq Stranded mRNA Sample Prep kit low-throughput configuration includes two sets of 48 samples/12 indexes, and the high-throughput configuration includes 96 samples/96 indexes.

Interested in stranded total RNA sample prep? TruSeq Stranded Total RNA Sample Preparation Kit

Get more information, protocols, and references on the RNA Resources Page

Learn more about TruSeq technology

Limited Use Label License:  This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc.  The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity).  The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product.  The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.


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TruSeq Stranded mRNA Sample Prep Kit
RS-122-2101
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MiSeq Reagent Kits v2

MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

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  • Get faster cycle times, longer reads and more output from improved chemistry
  • Dual surface imaging enables twice the number of reads compared to single surface imaging in v1
  • Extend your read lengths with the new 500-cycle kit
  • Choose the perfect number of cycles for your application (50, 300, or 500)

When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a new 500-cycle format to allow the longest read lengths on any Illumina sequencing system, as well as the popular 50- and 300-cycle kits. All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq system to validate compatibility with user-defined applications.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

 

MiSeq Reagent KitNo. of ReadsKit Size (cycles)Output (max.)2 × 75 Output2 × 150 Output2 × 250 Output
MiSeq Reagent Kit v2 15 M 50, 300, 500 7.5 Gb 2.25 Gb 4.5 Gb 7.5 Gb
MiSeq Reagent Micro Kit v2 4 M 300 1.2 Gb 600 Mb 1.2 Gb N/A
MiSeq Reagent Nano Kit v2 1 M 300, 500 500 Mb 150 Mb 300 Mb 500 Mb

 

Mb = megabases, Gb = gigabases, M = millions

 

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.


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MiSeq Reagent Kits v2
MS-102-2001
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TruSeq Small RNA Sample Preparation Kit

TruSeq Small RNA Sample Preparation Kits provide a simple, cost-effective solution for generating small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters. These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing microRNA discovery and profiling throughput to match Illumina's unparalleled sequencing output. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

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Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species.

Learn more about TruSeq technology.


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TruSeq Small RNA Sample Preparation Kit
RS-200-0012
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Nextera XT DNA Sample Preparation Kit

The Nextera XT DNA Sample Preparation Kit allows the preparation of sequencing-ready libraries for small genomes (bacteria, archaea, viruses), amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time.

“We run samples from cancer and somatic genomes all using Nextera XT, all with excellent results, with a short time of hands-on work, and a very good quality of data.” —Lab Manager, Educational Institution

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The industry's fastest sample prep, optimized for small genomes, PCR amplicons, and plasmids.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only a single nanogram, to support a wide array of input samples. Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, Nextera XT also includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing.  Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

PCR amplicons and small genomes can be easily prepared with Nextera XT and rapidly sequenced with MiSeq and NextSeq 500. See the interview and videos below to learn more about how researchers are using Nextera to accomplish studies never before possible.

Featured Researcher


Dr. Thomas Sutter, Ph.D.
“Nextera XT allows our researchers to spend less time making libraries and more time making discoveries.”

—Dr. Thomas Sutter
Director of the W. Harry Feinstone Center for Genomic Research at the University of Memphis

Read Interview »


Videos

Nextera XT Nextera XT DNA

Nextera XT DNA Sample Prep in the Lab
See a demonstration of Nextera XT DNA Sample Preparation in the lab. The fast and easy workflow allows researchers prepare sequencing-ready libraries for small genomes, PCR amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time.


MiSeq vs. Ion Torrent PGM MiSeq vs. Ion Torrent PGM

MiSeq vs Ion Torrent PGM in a Bacterial Research Lab
Join Dr. Tim Stinear for a presentation comparing genome sequencing of three reference bacteria of varying DNA composition with the MiSeq personal sequencer using Nextera library prep against the latest PGM chemistry and chips.


Nextera XT Nextera XT DNA

Nextera XT DNA Sample Prep Overview
Get an overview of Nextera XT DNA Sample Preparation kits, the industry's fastest and easiest method for preparing sequencing-ready libraries for small genomes, PCR amplicons, and plasmids.


Literature

Download the data sheet »

Please note that a Nextera XT Index kit is required to complete the protocol, regardless of the sample pooling level used for sequencing. Select the desired index kit using the "Add to Cart" function above.


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Nextera XT DNA Sample Preparation Kit
FC-131-1024
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TruSeq Nano DNA Sample Prep Kit

The TruSeq Nano DNA Sample Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted sample preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

  • Designed for low sample input
  • High coverage quality
  • Accelerated sample preparation

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Two kit types are available to accommodate a range of study designs: the TruSeq Nano DNA LT Sample Preparation Kit for low-throughput studies and the TruSeq Nano DNA HT Sample Preparation Kit for high-throughput studies. Although these kits are compatible with most Illumina sequencing systems, when used with HiSeq X Ten, only the high-throughput version of the kit (96 samples) is supported.

Use with limited DNA samples

The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (e.g., tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome.

These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Prepare libraries in less than a day

The proven TruSeq DNA sample preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. Optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp, the TruSeq Nano DNA kit is designed to match the ever-increasing read lengths of Illumina sequencing instruments. Master-mixed reagents, provided sample purification beads for cleanup and size selection, robust TruSeq indices, and optimized protocols contribute to the simplified workflow, requiring minimal hands-on time and few cleanup steps for processing large sample numbers.

Comparison of TruSeq DNA Sample Preparation Kits

SpecificationTruSeq Nano DNATruSeq DNA PCR-FreeTruSeq DNA
Description Based upon widely adopted TruSeq sample prep, with lower input and improved data quality Superior genomic coverage with radically reduced library bias and gaps Original TruSeq next-generation sequencing sample preparation method
Input quantity 100–200 ng 1–2 μg 1 μg
Includes PCR Yes No Yes
Assay time ~6 hours ~5 hours 1–2 days
Hands-on time ~5 hours ~4 hours ~8 hours
Target insert size 350 bp or 550 bp 350 bp or 550 bp 300 bp
Gel-Free Yes Yes No
Number of samples supported 24 (LT) or 96 (HT) samples 24 (LT) or 96 (HT) samples 48 (LT) or 96 (HT) samples
Supports enrichment No No Yes
Size-selection beads Included Included Not included
Applications Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies
Sample multiplexing 24 single indices or 96 dual-index combinations
Compatible Illumina sequencers NextSeq 500, HiSeq, HiScanSQ, Genome Analyzer, and MiSeq systems

 

 

To help you select the most appropriate version of the kit for your research, this webinar details the differences between the kits.
View the webinar - TruSeq: Comparison of Available DNA Sample Preparation Kits »


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TruSeq Nano DNA Sample Prep Kit
FC-121-4001
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Nextera DNA Sample Preparation Kit

Nextera DNA Sample Preparation Kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

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The industry's fastest sample prep.

MiSeq: Prep 1.5 hours, Sequence 4.5 hours, Analyze less than 2 hours

View the workflow »
Workflow shown using the Nextera Sample Preparation Kit, and 1 x 36 bp sequencing on MiSeq system.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

The TruSeq Dual Index Sequencing Primer Box is required for single-read and paired-end sequencing of Nextera libraries on HiSeq 1000/2000, HiScanSQ, and Genome Analyzer IIx instruments. These primers are required for non-indexed, single-indexed, and dual-indexed Nextera libraries.

Please note that a Nextera Index kit (FC-121-1011 or FC-121-1012) is required to complete the protocol, regardless of the sample pooling level used for sequencing.

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Nextera DNA Sample Preparation Kit
FC-121-1031
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TruSeq RNA Sample Preparation Kit v2

The TruSeq RNA Sample Preparation Kits provide a simple, cost-effective solution for generating mRNA-focused libraries from total RNA. The kits feature 24 unique indexes, delivering enhanced multiplex performance for processing up to 384 RNA-Seq samples on a single HiSeq 2500 run.

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Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Interested in stranded mRNA sample prep? TruSeq Stranded mRNA Sample Preparation Kit

Interested in stranded total RNA sample prep? TruSeq Stranded Total RNA Sample Preparation Kit

Get more RNA-Seq information, protocols and references on the RNA Resources Page

Learn more about TruSeq technology


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TruSeq RNA Sample Preparation Kit v2
RS-122-2001
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TruSeq DNA PCR-Free Sample Preparation Kit

TruSeq DNA PCR-Free Sample Preparation Kits provide simple, all-inclusive sample preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to whole-human genomes. The kits offer shortened gel-free workflows, the ability to sequence the most challenging regions, and the power to identify the greatest number of variants. Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

  • TruSeq DNA PCR-Free LT Sample Preparation Kits for low-throughput studies. Includes 24 single indexes.
  • TruSeq DNA PCR-Free HT Sample Preparation Kits for high-throughput studies. Includes 96 dual indexes pre-loaded in a 96-well plate.

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Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are traditionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content. The kits are tunable to a variety of read lengths and are supported on all of Illumina's sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high GC/AT rich regions.

Use PCR-free for faster protocols

The industry's most widely adopted sample preparation workflow for DNA sequencing is now enhanced. Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina's sequencing systems, the TruSeq DNA PCR-Free Sample Preparation Kit provides a range of enhancements to the industry's most widely adopted sample preparation workflow.

Kits for limited available DNA and exome enrichment

TruSeq Nano DNA Sample Prep is also available for efficient interrogation of samples with limited available DNA.

For enrichment, the Nextera Rapid Capture Exome and Expanded Exome assays provide a seamless end-to-end library preparation solution. These all-in-one sample preparation and exome enrichment kits allow researchers to identify coding variants 70% faster than any other method. An expanded kit increases coverage to UTRs and miRNA.

Comparison of TruSeq DNA Sample Preparation Kits

SpecificationTruSeq Nano DNATruSeq DNA PCR-FreeTruSeq DNA
Description Based upon widely adopted TruSeq sample prep, with lower input and improved data quality Superior genomic coverage with radically reduced library bias and gaps Original TruSeq next-generation sequencing sample preparation method
Input quantity 100–200 ng 1–2 μg 1 μg
Includes PCR Yes No Yes
Assay time ~6 hours ~5 hours 1–2 days
Hands-on time ~5 hours ~4 hours ~8 hours
Target insert size 350 bp or 550 bp 350 bp or 550 bp 300 bp
Gel-Free Yes Yes No
Number of samples supported 24 (LT) or 96 (HT) samples 24 (LT) or 96 (HT) samples 48 (LT) or 96 (HT) samples
Supports enrichment No No Yes
Size-selection beads Included Included Not included
Applications Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies
Sample multiplexing 24 single indexes or 96 dual-index combinations
Compatible Illumina sequencers NextSeq 500, HiSeq, HiScanSQ, Genome Analyzer, and MiSeq systems

 

 

To help you select the most appropriate version of the kit for your research, this webinar details the differences between the kits.
View the webinar - TruSeq: Comparison of Available DNA Sample Preparation Kits »


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TruSeq DNA PCR-Free Sample Preparation Kit
FC-121-3001
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TruSight One Sequencing Panel

Enabling labs to expand and streamline their sequencing portfolio, while managing costs.

  • Targets 4,813 genes associated with known clinical phenotypes
  • One workflow to follow and one procedure for managing genomic samples, increasing productivity, reducing handling errors, and decreasing laboratory costs

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The TruSight One sequencing panel provides clinical genomics research laboratories with an affordable solution for managing a diverse assay portfolio. Developed by Illumina, the TruSight One sequencing panel provides comprehensive coverage of > 4,800 clinically relevant genes. Laboratories can analyze all of the genes on the panel or choose to focus on a specific subset. Use of this panel enables expansion of existing menus, streamlining of workflows, or creation of an entire portfolio of sequencing options. The result is increased productivity, reduced handling errors, decreased laboratory costs, and consistent reporting.

The TruSight One sequencing panel focuses on the exonic regions harboring disease-causing mutations. It was designed to cover the most commonly ordered molecular assays, enabling labs to perform these on-site with one assay. Genomic targets were identified based on information in the Human Gene Mutation Database (HGMD Professional)1, the Online Mendelian Inheritance in Man (OMIM) catalog2, GeneTests.org3, Illumina TruSight panels4, and other commercially available sequencing panels. Combining data from these sources ensures that the TruSight One panel covers all genes currently reviewed in clinical research settings for a truly comprehensive screening assay.

For those needing bioinformatics capabilities, TruSight One comes with VariantStudio software, a simple, yet powerful tool for analysis, classification, and reporting of genomic variants. Using VariantStudio, labs can quickly and accurately extract and report on only the disease-relevant information of interest.

The TruSight One sequencing panel provides oligos targeting the identified regions of interest and reagents for sample preparation and enrichment. TruSight One is supported on the MiSeq, NextSeq, and HiSeq sequencing systems.

References

  1. Human gene mutation database (HGMD® Professional) (www.biobaseinternational.com/hgmd) from BIOBASE Corporation. The Human Gene Mutation Database: 2008 update. Stenson PD, Mort M, Ball EV, Howells K, Phillips AD, et al. (2009) Human gene mutation database (HGMD Professional) Genome Med. 1: 13. (www.hgmd.cf.ac.uk/ac/index.php)
  2. Online Mendelian Inheritance in Man (omim.org)
  3. GeneTests (www.genetests.org)
  4. Illumina TruSight Products (www.illumina.com/clinical/translational_genomics/panels.ilmn)
For Research Use Only. Note regarding biomarker patents and other patents unique to specific uses of products. Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.

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TruSight One Sequencing Panel
FC-141-1006
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TruSight Autism

Assists in the investigation of genomic features associated with autism.

  • Developed in collaboration with Dr. Jonathan Pevsner and team at Kennedy Krieger Institute
  • Targets 101 developmental delay genes linked specifically to autism

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TruSight Autism includes genes reported in the Online Mendelian Inheritance in Man (OMIM) database on autism; genes with recurrent mutations resulting in developmental delays1–3; genes with reported mutations as found in case studies involving developmental delay characteristics; and genes from summaries of autism-relevant genes (e.g., AutismKB4). Genes neighboring strong association signals were excluded in the absence of published reports of mutations.

The TruSight Autism sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Autism is compatible with TruSight Rapid Capture and is supported on the MiSeq, NextSeq, and HiSeq sequencing systems.

For Research Use Only.

References

  1. Marshall CR, Noor A, Vincent JB, Lionel AC, Feuk L, et al. (2008) Structural variation of chromosomes in autism spectrum disorder. Am J Hum Genet 82(2): 477-488.
  2. Talkowski ME, Rosenfeld JA, Blumenthal I, Pillalamarri V, Chiang C, et al. (2012) Sequencing chromosomal abnormalities reveals neurodevelopmental loci that confer risk across diagnostic boundaries. Cell 149(3): 525-537.
  3. Vendeweyer G and Kooy RF (2009) Balanced translocations in mental retardation. Hum Genet 126(1): 133-147.
  4. autism.cbi.pku.edu.cn

Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.


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TruSight Autism
TG-141-1003
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TruSight Cancer

Targeting genes previously linked to a predisposition towards cancer.

  • Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
  • Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

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TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatible with TruSight Rapid Capture and is supported on the MiSeq, NextSeq, and HiSeq sequencing systems.

For Research Use Only.

Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.


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TruSight Cancer
TG-141-1002
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TruSight Cardiomyopathy

Focusing on identifying inherited causes of cardiomyopathy.

  • Developed in collaboration with Dr. Heidi Rehm, Dr. Birgit Funke, and their team at the Laboratory for Molecular Medicine (LMM) and Partners Healthcare Center for Personalized Genetic Medicine (PCPGM), Harvard Medical School
  • Targets 46 genes linked to inherited cardiomyopathy 

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TruSight Cardiomyopathy targets genes linked to inherited cardiomyopathies, including Hypertrophic Cardiomyopathy (HCM), Dilated Cardiomyopathy (DCM), Restrictive Cardiomyopathy (RCM), Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC), Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT), and Left Ventricular Non-Compaction (LVNC). Additional content covers several syndromes, including Danon and Fabry disease, Barth syndrome, and Transthyretin Amyloidosis — all of which can present with isolated cardiomyopathy. Genes were chosen based on careful review of the literature and LMM's nine years of experience in genetic testing for inherited cardiomyopathies. 

The TruSight Cardiomyopathy sequencing panel includes custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cardiomyopathy is compatible with TruSight Rapid Capture and is supported on the MiSeq, NextSeq, and HiSeq sequencing systems.

For Research Use Only.

Note regarding biomarker patents and other patents unique to specific uses of products.
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.


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TruSight Cardiomyopathy
TG-141-1004
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TruSight Inherited Disease

Focusing on severe, recessive pediatric onset diseases.

  • Developed in collaboration with Dr. Stephen Kingsmore and team at Children's Mercy Hospital (CMH) for Pediatric Genomic Medicine, Dr. Carol Saunders at CMH, and Dr. Hilger Ropers at the Max Planck Institute
  • Targets 552 genes, including coding exons, intron-exon boundaries, and regions known to harbor pathogenic mutations 

More Details +

TruSight Inherited Disease was initially based on a 448 disease panel designed for preconception carrier testing for severe, recessive childhood diseases published by Dr. Kingsmore and team in Science Translational Medicine1. The original content was revised by Dr. Saunders, FACMG, at CMH (following ACMG guidelines for testing ultra-rare genetic diseases) to reflect the needs of medical geneticists with a primary focus on severe recessive diseases with childhood onset. Intellectual disability genes were added by Dr. Ropers.

The TruSight Inherited Disease sequencing panel set includes custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Inherited Disease is compatible with TruSight Rapid Capture and is supported on the MiSeq, NextSeq, and HiSeq sequencing systems.

For Research Use Only.

References

  1. Saunders CJ, Miller NA, Soden SE, Dinwiddie DL, Noll A, et al. (2012) Rapid whole-genome sequencing for genetic disease diagnosis in neonatal intensive care units. Sci Transl Med. 4: 154ra135.  

Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.


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TruSight Inherited Disease
TG-141-1005
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TruSight Myeloid Sequencing Panel

The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic mutations in myeloid malignancies.

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The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:

  • Acute myeloid leukemia (AML)
  • Myelodysplastic syndrome (MDS)
  • Myeloproliferative neoplasms (MPN)
  • Chronic myelogenous leukemia (CML)
  • Chronic myelomonocytic leukemia (CMML)
  • Juvenile myelomonocytic leukemia (JMML)

The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution for profiling liquid tumors. View the gene list »

TruSight Myeloid libraries are ideally suited to run on the MiSeq and perform alignment and variant calling with the MiSeq Reporter Somatic Variant Caller. Filtering and annotation can then be performed using Illumina’s annotation and filtering software, VariantStudio.

ABL1 CEBPA HRAS MYD88 SF3B1
ASXL1 CSF3R IDH1 NOTCH1 SMC1A
ATRX CUX1 IDH2 NPM1 SMC3
BCOR DNMT3A IKZF1 NRAS SRSF2
BCORL1 ETV6/TEL JAK2 PDGFRA STAG2
BRAF EZH2 JAK3 PHF6 TET2
CALR FBXW7 KDM6A PTEN TP53
CBL FLT3 KIT PTPN11 U2AF1
CBLB GATA1 KRAS RAD21 WT1
CBLC GATA2 MLL RUNX1 ZRSR2
CDKN2A GNAS MPL SETBP1  

Product shipping in May.



TruSight Myeloid Sequencing Panel
FC-130-1010
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TruSight Rapid Capture

Prepare target sequencing libraries 70% faster.

TruSight Rapid Capture are all-in-one kits for library preparation and enrichment that allow researchers to prepare TruSight target sequencing libraries 70% faster than any other method. TruSight Rapid Capture allows researchers to maximize the productivity of their lab personnel and Illumina sequencing technology, enabling library preparation and enrichment-based sequence capture for up to 96 samples in only 1.5 days.

More Details +
  • Integrates library preparation and enrichment steps
  • Offers a fast, streamlined, and optimized workflow
  • Delivers fully enriched libraries for up to 96 samples in just 1.5 days

TruSight Rapid Capture leverages the speed of Nextera library preparation technology. By eliminating the need for mechanical DNA fragmentation and introducing a unique multiplex pre-enrichment sample pooling, TruSight Rapid Capture reduces hands-on time and saves at least one full day over all other currently available enrichment workflows.

TruSight Rapid Capture is compatible with MiSeq Reagent Kits and is supported on both the MiSeq and HiSeq systems.

TruSight Rapid Capture Kits Information

TG Catalog No.*Catalog No.Samples/KitEnrichment Rxns/KitIndices/Kit
TG-140-1101 FC-140-1101 8 8 1
TG-140-1102 FC-140-1102 8 4 2
TG-140-1103 FC-140-1103 16 4 4
TG-140-1104 FC-140-1104 48 4 24
TG-140-1105 FC-140-1105 96 8 24
TG-140-1106 FC-140-1106 288 24 96

* TG-labeled consumables include features intended to help customers reduce the frequency of revalidation. They are available only under supply agreement and require customers to provide a binding forecast. TruSight content sets are available for evaluation purposes prior to executing a supply agreement. Please contact your account manager for more information.

For Research Use Only.


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FC-140-1101
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TruSight Tumor

Access a deeper view of variation in solid tumors.

The TruSight Tumor sequencing panel takes a deeper view of variation in solid tumors including lung, colon, melanoma, gastric and ovarian. This enables clinical researchers to identify low-frequency variation across 26 genes for a more comprehensive view of somatic variation.

More Details +
  • Highly accurate variant analysis at limit of detection below 5% variant allele frequency across 174 amplicons, with 1000x minimum coverage of each region
  • Exceptional sample success rates with minimal DNA input for accurate base calling, even in degraded FFPE samples
  • Coverage of complete exons for analysis of molecular heterogeneity in highly relevant content selected from CAP1 and NCCN2 guidelines, and late stage clinical trials3

The TruSight Tumor sequencing panel provides amplicon-based library preparation reagents, DNA QC, sample indices and oligos targeting identified regions of interest. Sufficient product is supplied for 48 samples. TruSight Tumor is supported on the MiSeq, HiSeq, and NextSeq systems.

Illumina Next-Gen Sequencing: Think Big, Start Small

Kenneth Bloom, MD, FCAP, Chief Medical Officer at Clarient (GE Healthcare), discusses the practical aspects of how next-generation sequencing will be applied in the clinical laboratory.
Watch the video »

Literature

TruSight Tumor Data Sheet (PDF)

Generating Sequencing Libraries from FFPE Samples White Paper (PDF)

Amplicon - DS Somatic Variant Caller Technical Note (PDF)

Illumina VariantStudio Data Sheet (PDF)

TruSight Portfolio: New Possibilities for Next-Generation Sequencing

Scientific Posters

AMP2013: A multi-institutional study: Evaluation of a novel technique that uses strand-specific information to increase accuracy in a next-generation sequencing assay to identify somatic DNA variants (PDF)

ASCO2013: A novel approach that improves detection of somatic DNA variants in solid tumors by next-generation sequencing from FFPE samples (PDF)

ESHG2013: A novel technique that distinguishes low-level somatic DNA variants from FFPE-induced artifacts in solid tumors by next-generation sequencing (NGS) (PDF)

For Research Use Only.

References

  1. Lindeman NI, Cagle PT, Beasley MB, Chitale DA, et al. 2013 Molecular Testing Guideline for Selection of Lung Cancer Patients for EGFR and ALK Tyrosine Kinase Inhibitors. Archives of Pathology & Laboratory Medicine Vol 137 Issue 4
  2. NCCN:www.nccn.org/professionals/physician_gls/f_guidelines.asp#site
  3. Clinical trials: www.clinicaltrials.gov

Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.


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TruSeq Custom Amplicon v1.5

TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. With targeted resequencing, researchers can focus interrogation on key regions of genomic interest. The assay allows researchers to sequence up to 1536 amplicons in a single reaction using a simple workflow. This highly targeted approach offers unparalled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

More Details +

Requiring as little as 50 ng of input gDNA, TruSeq Custom Amplicon can be used with a wide range of sample types. The ability to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling researchers to fully benefit from the MiSeq's output capabilities.

TruSeq Custom Amplicon v1.5 provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stability. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

TruSeq Custom Amplicon offers a fully integrated DNA-to-data solution, including convenient probe design and ordering using DesignStudio, an easy-to-use online software tool that provides dynamic feedback to optimize design and region coverage. Automated data analysis using the Illumina Amplicon Viewer software allows researchers to easily review project data, including variants detected, across multiple MiSeq runs.

Start a new TruSeq Custom Amplicon project »
Access the TruSeq Custom Amplicon estimator »
Learn more about DesignStudio »


Interview

Interview with Robert Daber

Dr. Daber uses TruSeq Custom Amplicon for genetic testing that can inform prognosis and therapeutic choices.
Access PDF »

Workflow

Prepare and enrich up to 384 amplicons per sample and 96 samples per plate simultaneously

The TruSeq Custom Amplicon Assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
View the workflow »

Videos

TruSeq Custom Amplicon TruSeq Custom Amplicon play button

An introduction to TruSeq Custom Amplicon (TSCA), the fastest and easiest-to-use multiplexed amplicon assay optimized for with the MiSeq system. Target hundreds of genes with simple, customized design. Detect rare somatic mutations below 5%, even in difficult FFPE samples. Prepare and sequence ~150,000 amplicons on the MiSeq system in less than two days, with automated analysis.



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TruSeq Amplicon - Cancer Panel

The TruSeq Amplicon - Cancer Panel (TSACP) is a highly multiplexed targeted resequencing assay for detecting somatic mutations. Leveraging the long paired-end read capability, speed, and high data quality of the MiSeq system, entire projects can now be accomplished in days instead of months.

More Details +

TSACP enables highly sensitive mutation detection within important cancer-related genes, including BRAF, KRAS, and EGFR. Mutations in these genes are linked to many cancers, including melanoma, colorectal, ovarian, and lung cancer. The unique ability of this assay to screen precious FFPE samples for these important variants will unlock a wealth of genomic information for many tumor types.

Learn more about detecting somatic mutations in cancer.

Genes in the TruSeq Amplicon - Cancer Panel

ABL1 EGFR GNAS MLH1 RET
AKT1 ERBB2 HNF1A MPL SMAD4
ALK ERBB4 HRAS NOTCH1 SMARCB1
APC FBXW7 IDH1 NPM1 SMO
ATM FGFR1 JAK2 NRAS SRC
BRAF FGFR2 JAK3 PDGFRA STK11
CDH1 FGFR3 KDR PIK3CA TP53
CDKN2A FLT3 KIT PTEN VHL
CSF1R GNA11 KRAS PTPN11
CTNNB1 GNAQ MET RB1

Download the manifest file. (MyIllumina login required)

Watch the MiSeq Webinar

How next-gen sequencing is revolutionizing cancer diagnoses
Join Dr. Andrew Fellowes of the Peter MacCallum Cancer Centre on April 26, as he discusses how his team uses the MiSeq system and TruSeq Amplicon - Cancer Panel for comprehensive profiling of tumor samples for a wider range of mutations than is possible with traditional technologies.

View the webinar »

Workflow

TruSeq Custom Amplicon Cancer Panel Workflow

TSACP provides a streamlined workflow, including an integrated quality control assay for DNA from formalin-fixed, paraffin-embedded (FFPE) samples, simple bead-based sample normalization, automated cluster generation and paired-end sequencing, and on-instrument data analysis.
View the TruSeq Amplicon - Cancer Panel workflow »

Literature

TruSeq Amplicon - Cancer Panel Data Sheet

Somatic Variant Caller Technical Note


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TruSeq Targeted RNA Expression Kits

TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation for the MiSeq system. Overcoming significant challenges in cost and workflow, TruSeq Targeted RNA assays deliver a fully integrated solution, including convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

More Details +

User-defined panels of 12-1,000 assays can be created to target genes, isoforms, splice junctions, cSNPs, and fusion genes with the ability to multiplex up to 384 samples. Unleash the power of sequencing-based gene expression profiling, and generate data equivalent of up to 25,000 individual RT-PCR assays in a single MiSeq run.*

TruSeq Targeted RNA Fixed Panels

For the ultimate in flexibility, custom content can be added to fully optimized, experimentally validated TruSeq Targeted RNA fixed panels focused on cell pathways and disease states.

 

Start a project

Start a new TruSeq Targeted RNA Expression project in DesignStudio, a web-based design tool for custom targeting projects. Start Now »

 

For Research Use Only.

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003


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Nextera Rapid Capture Exome and Expanded Exome Kits

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for sample preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 37 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing. An expanded kit increases coverage to UTRs and miRNA.

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Identify coding variants up to 70% faster than any other method
Nextera Rapid Capture Exome allows researchers to maximize the productivity of their lab personnel and Illumina sequencing technology, enabling sample prep and exome enrichment for up to 96 samples in only 1.5 days. Rapid sequencing with the HiSeq 2500 or NextSeq 500 systems allow experiments to go from DNA sample to data in less than 3 days, dramatically faster than all other technologies. Nextera Rapid Capture Exome kits deliver high enrichment rates, coverage uniformity and reproducibility. A variety of Nextera Rapid Capture Exome kits have been designed to allow optimal alignment with Illumina sequencing systems. This array of kit options provides exceptional flexibility in study design, and enables projects from one to hundreds of exomes per run.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome is the perfect solution when both coding variants and regulatory regions are of interest. Genome-wide association studies suggest that > 80% of disease-associated variants fall outside coding regions. Analysis of these regions enables researchers to discover variants that affect gene function, at a more affordable price than whole-genome sequencing.

Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More »

Featured Researcher

Nicholas Navin, Ph.D.

“Tools like the Nextera Rapid Capture Exome kit are allowing researchers to get a whole new view of tumors and their genetic diversity and complexity.”
—NICHOLAS NAVIN, Ph.D.
Assistant Professor in the Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas
Read the case study »


Video

Nextera Rapid Capture Exome and Expanded Exome KitsVNextera Rapid Capture Exome and Expanded Exome Kits

Nextera Rapid Capture Exome and HiSeq 2500


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Coverage Details

Nextera Rapid Capture Exome Coverage Details

To help you select the most appropriate kit for your research, this table details the differences in coverage provided by Nextera Rapid Capture Exome and Expanded Exome.

Data

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome sample prep kit. View the exome sequencing data »


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TruSeq Stranded Total RNA with Ribo-Zero Plant

TruSeq Stranded Total RNA Sample Preparation Kits with Ribo-Zero Plant deliver the clearest, most complete view of the plant transcriptome.

More Details +

High-quality whole-transcriptome analysis with precise strand information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. These kits couple the benefits of TruSeq RNA preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable end-to-end solution for whole-transcriptome analysis.

Efficient ribosomal reduction across a range of plant species, study designs

TruSeq Stranded Total RNA with Ribo-Zero Plant kits enable the rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. They have been validated for use in multiple species including Arabidopsis thaliana, rice, and maize and will provide efficient rRNA removal in a broad range of additional species.*

Each kit is available in low-throughput (LT) and high-throughput (HT) configurations (48 samples / 12 of 24 total indexes in Sets A and B and 96 samples, 96 unique dual-index pairs, respectively) and with protocols for the preparation of both small and large numbers of samples.

*For information on a particular species of interest, please contact Illumina Technical Support.


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PhiX Control v3

PhiX Control v3 is a reliable, adapter-ligated library used as a control for Illumina sequencing runs. The library is derived from the small, well-characterized PhiX genome, offering several benefits for sequencing and alignment.

More Details +

It can be rapidly aligned for estimating relevant SBS metrics such as phasing, prephasing, and error rate. Depending on the application PhiX Control v3 can be used as a high-level spike in for unbalanced (CG or AT biased) samples, a low-level spike in, or as a dedicated control lane alongside low diversity samples.  It is also an excellent control for troubleshooting cluster generation problems, allowing you to quickly determine if an error is related to sample preparation. The versatile PhiX Control v3 can be used in diverse applications to add value to your workflow and increase confidence in your results.


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Nextera Rapid Capture Custom Enrichment Kit

Nextera Rapid Capture Custom Enrichment provides unparalleled access to genomic regions of interest. This custom assay allows researchers to sequence precious samples faster and more efficiently than ever before, using as little as 50 ng of input DNA. The kit offers add-on functionality to refine content over time, or add regions of unique interest to established panels like Nextera Rapid Capture Exome or TruSight content sets. Nextera Rapid Capture Custom Enrichment allows researchers to:

  • Generate ~0.5 – 15 Mbps of custom content in a single enrichment assay
  • Evolve a custom assay over time with add-on capability
  • Design a preferred assay using an intuitive, user-friendly, online tool

More Details +

Rapidly interrogate key areas of specific research
With Nextera Rapid Capture Custom Enrichment, researchers can rapidly interrogate those portions of the human genome most important to their project. They can densely pack hybridization probes into a region as small as 0.5 Mbps, or space them more liberally to enrich up to 15 Mbps of fully custom content. Nextera Rapid Capture Custom Enrichment offers maximum flexibility to address specific needs.

Increase genomic regions enriched by the assay
Add-on functionality allows researchers to periodically increase those regions of the genome enriched by the custom assay. Researchers can start from our pre-defined panels, such as the Nextera Rapid Capture Exome and add in regions of particular interest, or start from an existing custom design and capitalize on new discoveries. For example, a project can start with existing exome content and add in important non-coding content.

Explore design options prior to order
Illumina's DesignStudio is a richly featured, intuitive, and fully supported online tool for creating custom assay designs. This free tool is available to anyone with a MyIllumina account. Researchers can add targets or regions of interest, designate what should be included or excluded from a final design, optimize probe density, and explore other design options all before committing to a final order. Start a Project Now »

Need a MyIllumina account? Register for a Free Account »


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Nextera Mate Pair Sample Preparation Kit

Illumina's Nextera Mate Pair Sample Preparation Kit offers the only gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement. It is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation — even in the most complex genomes such as those derived from cancer.

  • Sequence precious samples when limited DNA is available
  • Prepare sequencing-ready libraries in less than two days
  • Generate highly diverse libraries with premier data quality

More Details +

Requires 10 times less DNA input than other protocols

Both gel-free and gel-plus protocols are available. The gel-free protocol allows mate pair sequencing when limited DNA is available:

  • Designed for applications such as de novo assembly of small genomes and detection of structural variation
  • The industry's only gel-free protocol with such low DNA input requirements

The gel-plus protocol is ideal for challenging mate pair applications, such as de novo assembly of complex genomes and detection of structural variation.

Fast and simple mate pair workflow

Inclusion of the TruSeq DNA Sample Preparation master-mixed reagents in the Nextera Mate Pair kit reduces the number of assay steps, thereby reducing the hands-on time required.

  • Reduction in pipetting steps simplifies workflow and reduces sample loss
  • Gel-free protocol option and on-bead reactions simplify purification steps and shorten protocol hands-on time

Increased library diversity and data quality

The Nextera Mate Pair Sample Preparation Kit provides identifiable junction sequences for accurate identification of the two halves of the mate pair fragment.

  • Only biotinylates DNA molecules at fragmentation sites, avoiding troublesome internal biotinylation
  • Allows for the creation of millions of unique fragments
  • Increased library diversity generates fewer duplicate reads and premier data quality

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TruSeq ChIP Sample Prep Kit

TruSeq ChIP Sample Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome. This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

More Details +

TruSeq ChIP Sample Preparation Kits are compatible with all Illumina sequencers. Offering the proven data quality and ease of use of TruSeq DNA sequencing, they provide a streamlined ChIP-Seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time.

The kits have a low DNA input requirement and are compatible with a range of sample sources. In addition, they offer more robust multiplex sequencing with 24 unique indexes, allowing researchers to optimize the distribution of sequencing output across samples based on read depth requirements. 


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TruSeq Targeted RNA Expression Apoptosis Panel

The TruSeq Targeted RNA Expression Apoptosis Panel is a predesigned gene-expression profiling solution for studying apoptosis, or programmed cell death. The panel includes assays targeting 117 commonly studied pro-apoptotic and anti-apoptotic genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 117
Suggested # of Samples/MiSeq Run 214*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Apoptosis Panel

This table displays apoptosis-related genes represented in the TruSeq Targeted RNA Expression Apoptosis Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression Apoptosis Panel Gene List
ABL1 BCL2 BIRC6 CASP2 CFLAR FAS LTA PIK3CA TNF TP53I3
AIFM1 BCL2L11 BIRC7 CASP3 CIDEA FASLG LTB PIK3R2 TNFRSF10A TP73
AKT1 BCL2L13 BNIP2 CASP4 CIDEB HIP1 LTBR PTEN TNFRSF10B TRADD
APAF1 BCL2L2 BNIP3 CASP5 CRADD HMGB1 MCL1 RELA TNFRSF10D TRAF2
AVEN BCL3 BNIP3L CASP6 CYCS HRK MYC RIPK1 TNFRSF1A TRAF3
BAD BCLAF1 BOK CASP7 DAPK1 HSP90B1 NAIP RIPK2 TNFRSF1B TRAF5
BAG1 BFAR BRAF CASP8 DAXX IGF1R NFKB1 SOCS2 TNFRSF21 TRAF6
BAG3 BID CARD6 CASP8AP2 DEDD IKBIP NFKB2 SOCS3 TNFRSF9 TRAF7
BAG4 BIK CARD9 CASP9 DEDD2 IKBKB NFKBIA STAT1 TNFSF10 XIAP
BAK1 BIRC2 CASP1 CD27 DFFA IKBKG NOD1 STAT5A TNFSF8  
BAX BIRC3 CASP10 CD40 DIABLO IL10 NOL3 STAT5B TP53  
BCL10 BIRC5 CASP14 CD70 FADD IL1A PEA15 TBK1 TP53BP2  


TruSeq Targeted RNA Expression Apoptosis Panel
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TruSeq Targeted RNA Expression Cardiotoxicity Panel

The TruSeq Targeted RNA Expression Cardiotoxicity Panel is a predesigned gene-expression profiling solution for studying cellular pathways affected by cardiotoxic compounds or stress. The panel includes assays targeting 76 genes used as markers of the cardiotoxic response. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 76
Suggested # of Samples/MiSeq Run 329*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Cardiotoxicity Panel

This table displays cardiotoxicity-related genes represented in the TruSeq Targeted RNA Expression Cardiotoxicity Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression Cardio Panel Gene List
ABHD2 CCR1 GJA1 NEXN PSMD7 TGFB2 VIM
ABRA CD14 GPM6A NFIB PUM2 THRAP3 WIPI1
ACTA1 CFD HAMP PDK4 PVR TIAM1 ZNF148
AIFM1 COL15A1 HSPA2 PKN2 RBM3 TIMP1 ZNF23
AK3 COL3A1 HSPH1 PLA2G4A REG3G TUBB6  
ASH1L CREM IFT20 PLAU RND1 TXNIP  
ATP5J CSNK2A2 IGFBP5 PLN RPS6KB1 UBA5  
BCAT1 DUSP8 IL6 POSTN SERPINE1 UBXN2A  
BGN EGR1 ITPR2 PPBP SIK1 UCK2  
BSN FCGR2B KBTBD10 PPP1R14C SLC4A3 UCP1  
BTG2 FHL1 KBTBD5 PRKAB2 SPP1 VCAN  
CCL7 FOSL1 MCM6 PSMA2 TCF4 VEGFA  


TruSeq Targeted RNA Expression Cardiotoxicity Panel
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TruSeq Targeted RNA Expression Cell Cycle Panel

The TruSeq Targeted RNA Expression Cell Cycle Panel is a predesigned gene-expression profiling solution for studying the cell cycle. The panel includes assays targeting 63 genes involved in all phases of the cell cycle as well as DNA replication. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 63
Suggested # of Samples/MiSeq Run 384*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Cell Cycle Panel

This table displays cell cycle-related genes represented in the TruSeq Targeted RNA Expression Cell Cycle Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression Cell Cycle Panel Gene List
ABL1 CCNE1 CDK6 E2F4 PER2 TIMELESS
ANAPC2 CCNG2 CDK7 GADD45A PER3 TP53
ATM CCNH CDK8 MAD2L1 RAD51 WEE1
ATR CDC16 CDKN1A MAD2L2 RB1  
BRCA1 CDC20 CDKN1B MCM2 RBBP8  
BRCA2 CDC25A CHEK1 MCM3 RBL1  
CCNA2 CDC25C CHEK2 MCM4 RBL2  
CCNB1 CDC34 CREB3 MCM5 SKP2  
CCNB2 CDC6 CRY1 MDM2 SMAD4  
CCND1 CDK1 CRY2 MNAT1 TFDP1  
CCND2 CDK2 CUL1 MRE11A TFDP2  
CCND3 CDK4 E2F1 PER1 TGFB1  


TruSeq Targeted RNA Expression Cell Cycle Panel
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TruSeq Targeted RNA Expression Hedgehog Panel

The TruSeq Targeted RNA Expression Hedgehog Panel is a predesigned gene-expression profiling solution for studying hedgehog signaling. The panel includes assays targeting 76 genes involved in upstream and downstream signal transduction, as well as transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 76
Suggested # of Samples/MiSeq Run 329*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Hedgehog Panel

This table displays hedgehog signaling-related genes represented in the TruSeq Targeted RNA Expression Hedgehog Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression Hedgehog Panel Gene List
BCL2 CTNNB1 GLI1 MTSS1 RAB23 WNT10A WNT8A;
BMP2 DHH GLI2 NF2 RUNX2 WNT10B WNT8B;
BMP4 DISP1 GLI3 NPC1 SFRP1 WNT11 WNT9A;
BMP5 DISP2 GSK3B NUMB SHH WNT16 ZIC1;
BMP6 ERBB4 HHAT OTX2 SMO WNT2B  
BMP7 FAT4 HHIP PRKACA STK3 WNT3  
BMP8B FBXW11 IFT52 PRKACB STK36 WNT3A  ;
BOC FGF9 KCTD11 PTCH1 SUFU WNT4  
BTRC FGFR3 LATS1 PTCH2 TP53 WNT5A  
CDON FKBP8 LATS2 PTCHD1 VEGFA WNT5B  
CSNK1A1 FOXE1 LRP2 PTCHD2 WIF1 WNT6  
CSNK1E FRMD6 MAPK1 PTCHD3 WNT1 WNT7B  


TruSeq Targeted RNA Expression Hedgehog Panel
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TruSeq Targeted RNA Expression Neurodegeneration Panel

The TruSeq Targeted RNA Expression Neurodegeneration Panel is a predesigned gene-expression profiling solution for studying neurodegenerative and neurotoxic pathways such as those implicated in Alzheimer’s Disease. The panel includes assays targeting 77 genes involved in these pathways. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 77
Suggested # of Samples/MiSeq Run 325*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Neurodegeneration Panel

This table displays neurodegeneration-related genes represented in the TruSeq Targeted RNA Expression Neurodegeneration Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression Neuro Panel Gene List
A2M APLP2 CASP7 GAP43 INSR PLG SNCA
ABCA1 APOA1 CDK5 GNB1 LPL PRKCA SNCB
ABL1 APOE CHAT GRIN1 LRP1 PRKCB SOD2
ACHE APP CLU GSK3B LRP6 PRKCD TACR1
ADAM10 BACE1 CTSB HSD17B10 LRP8 PRKCE TP53
ADAM9 BACE2 CTSD HSP90AA1 MAPT PRKCG  
APBA1 BCHE DRD2 HSPA5 MPO PRKCI  
APBA3 BDNF EP300 HTR1A NAE1 PRKCQ  
APBB1 CAMK2A ERN1 HTR3A NCSTN PRKCZ  
APBB2 CAPN1 FAS IDE PKP4 PSEN1  ;
APH1A CASP3 FASLG IL1A PLAT PSEN2  
APLP1 CASP4 GAL INS PLAU SERPINA3  


TruSeq Targeted RNA Expression Neurodegeneration Panel
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TruSeq Targeted RNA Expression NFκB Panel

The TruSeq Targeted RNA Expression NFκB Panel is a predesigned gene-expression profiling solution for studying NFκB signaling. The panel includes assays targeting 105 genes involved in upstream and downstream signal transduction, as well as NFκB transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 105
Suggested # of Samples/MiSeq Run 238*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - NFκB Panel

This table displays NFκB signaling-related genes represented in the TruSeq Targeted RNA Expression NFκB Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression NFKB Panel Gene List
AKT1 CCL2 CSF1 GADD45B IL1RN MYC REL TICAM1 TNFSF10
ATF1 CCL22 CSF2 HMOX1 IL2 MYD88 RELA TLR1 TNFSF14
BCL10 CCL5 CSF3 ICAM1 IL6 NFKB1 RELB TLR2 TP53
BCL2L1 CCR5 CXCL1 IFNA1 IL8 NFKB2 RHOA TLR3 TRADD
BCL3 CD27 CXCL10 IFNB1 IRAK1 NFKBIA RIPK1 TLR6 TRAF2
BIRC2 CD40 CXCL2 IFNG IRAK2 NFKBIB SELE TLR9 TRAF3
BIRC3 CD69 CXCL9 IKBKE IRF1 NFKBIE SELP TNF TRAF6
C3 CD80 EGFR IKBKG LTBR NR4A2 SOD2 TNFAIP3 VCAM1
CARD11 CD83 EGR1 IL12B MALT1 PDGFB STAT1 TNFRSF10A XIAP
CASP1 CDKN1A ELK1 IL15 MAP2K6 PLAU STAT3 TNFRSF10B  
CASP8 CFLAR FASLG IL1B MAP3K1 PTGS2 STAT5B TNFRSF1A  
CCL11 CHUK FOS IL1R1 MMP9 RAF1 TBK1 TNFRSF1B  


TruSeq Targeted RNA Expression NFκB Panel
RT-201-1008
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TruSeq Targeted RNA Expression p53 Panel

The TruSeq Targeted RNA Expression p53 Panel is a predesigned gene-expression profiling solution for studying p53 signaling. The panel includes assays targeting 52 genes involved in upstream and downstream signal transduction, as well as transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

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Specifications
Number of Genes Targeted 52
Suggested # of Samples/MiSeq Run 384*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - p53 Panel

This table displays p53 signaling-related genes represented in the TruSeq Targeted RNA Expression p53 Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression p53 Panel Gene List
APAF1 CASP8 E2F3 MLH1 TNFRSF10B
ATM CASP9 ERBB2 NFKB1 TP53
ATR CCND1 FASLG NFKBIA TP63
BAI1 CCNE1 GADD45A PCNA TP73
BCL2 CCNG1 GML PPM1D  
BCR CDK1 HDAC1 PTEN  
BID CDK4 HRAS RB1  
BIRC5 CDKN1A JAK2 SERPINB5  
BRCA1 CHEK1 KAT2B SESN2  
BRCA2 CHEK2 KITLG SIRT1  
BTG2 CTNNB1 MDM2 SRC  
CASP2 E2F1 MDM4 TNF  


TruSeq Targeted RNA Expression p53 Panel
RT-201-1007
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TruSeq Targeted RNA Expression Cytochrome p450 Panel

The TruSeq Targeted RNA Expression Cytochrome p450 Panel is a predesigned gene-expression profiling solution for studying Cytochrome p450 (CYP) genes. The panel includes assays targeting 28 cytochrome P450 genes involved in drug and toxin metabolism. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

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Specifications
Number of Genes Targeted 28
Suggested # of Samples/MiSeq Run 384*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Cytochrome p450 Panel

This table displays cytochrome p450-related genes represented in the TruSeq Targeted RNA Expression Cytochrome p450 Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

Table: TruSeq Targeted RNA Expression p450 Panel Gene List
CYP11A1 CYP2C9 CYP51A1
CYP19A1 CYP2J2 CYP7A1
CYP1A1 CYP2R1 CYP7B1
CYP1A2 CYP3A4 PTGIS
CYP24A1 CYP3A43  
CYP26B1 CYP4A11  
CYP27A1 CYP4F12  
CYP27B1 CYP4F2  
CYP27C1 CYP4F3  
CYP2B6 CYP4F8  
CYP2C19 CYP4X1  
CYP2C8 CYP4Z1  


TruSeq Targeted RNA Expression Cytochrome p450 Panel
RT-201-1006
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TruSeq Targeted RNA Expression Stem Cell Panel

The TruSeq Targeted RNA Expression Stem Cell Panel is a predesigned gene-expression profiling solution for studying induction, differentiation and maintenance of a variety of stem cell types. The panel consists of assays targeting 100 genes, including stem cell markers, differentiation markers, pluripotency markers, cytokines and growth factors. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

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Specifications
Number of Genes Targeted 100
Suggested # of Samples/MiSeq Run 250*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Stem Cell Panel

This table displays stem cell-related genes represented in the TruSeq Targeted RNA Expression Stem Cell Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

Table: TruSeq Targeted RNA Expression Stem Cell Panel Gene List
ABCG2 CCND1 COL1A1 EP300 GATA6 KRT15 NOTCH3 RB1 TCF7
ACTC1 CCND2 COL2A1 FGF1 GDF3 LIFR NOTCH4 S100B TERT
ADAR CCNE1 CREBBP FGF4 GLI1 MME NUMB SDAD1 WNT1
ALDH1A1 CD3D CTNNA1 FGFR1 GLI2 MYC OLIG2 SMAD2 WT1
ALDH2 CD4 CTNNB1 FOXA2 GLI3 MYOD1 PAX6 SMAD4  
APC CD44 CXCL12 FZD2 HDAC2 NANOG PDX1 SMO  
AXIN1 CD8A DHH FZD3 IGF1 NCAM1 POU5F1 SOX1  
BMP1 CD8B DLL1 FZD5 IL6ST NCSTN PPARD SOX2  
BMP2 CDC42 DLL3 FZD6 ISL1 NEUROD1 PPARG SP1  
BMP3 CDH1 DNMT3B FZD7 JAG1 NFATC1 PSEN1 STAT3  
BTRC CDH2 DTX2 FZD8 JUND NOTCH1 PSEN2 SUFU  
CCNA2 CDX2 DVL1 FZD9 KAT2A NOTCH2 PSENEN T  

For Research Use Only.



TruSeq Targeted RNA Expression Stem Cell Panel
RT-201-1005
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TruSeq Targeted RNA Expression Wnt Panel

The TruSeq Targeted RNA Expression Wnt Panel is a predesigned gene-expression profiling solution for studying Wnt signaling. The panel includes assays targeting 93 genes involved in upstream and downstream signal transduction, as well as transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

More Details +
Specifications
Number of Genes Targeted 93
Suggested # of Samples/MiSeq Run 269*
Input Requirement 50 ngs Total RNA**
Species Supported Human
Add-on capacity 12-1,000 targets
Time to Answer 1.5 days

*Assumes MiSeq Reagent v3 and 25M reads
**May vary with expression level, target plexity and sample quality

 

Genes in the TruSeq Targeted RNA - Wnt Panel

This table displays Wnt-related genes represented in the TruSeq Targeted RNA Expression Wnt Panel. View Table »

Don’t see your favorite gene? Go to DesignStudio to add content to this panel.

For more information about TruSeq Targeted RNA Expression Kits, see the overview.

 

For Research Use Only.

Table: TruSeq Targeted RNA Expression Wnt Panel Gene List
ABCB1 CSNK1A1 FGF20 FZD8 MMP2 PRICKLE1 TCF7L2 WNT3
AES CSNK2A1 FGF4 FZD9 MMP7 PYGO1 TLE1 WNT3A
APC CTBP1 FGF9 GSK3A MMP9 RHOA TWIST1 WNT4
AXIN1 CTNNB1 FOSL1 GSK3B MYC RUNX2 VANGL2 WNT5A
AXIN2 CTNNBIP1 FOXN1 ID2 NANOG RUVBL1 VEGFA WNT5B
BCL9 CXXC4 FRZB IL6 NFATC1 SFRP1 WIF1 WNT6
BIRC5 DAAM1 FZD2 KREMEN1 NKD1 SFRP2 WISP1 WNT7B
BMP4 DKK1 FZD3 LEF1 NLK SFRP4 WNT1 WNT8A
BTRC DVL1 FZD4 LRP5 PITX2 SOX17 WNT10A WNT9A
CCND1 DVL2 FZD5 LRP6 PLAUR SOX9 WNT11  
CCND2 EP300 FZD6 MAPK8 PORCN TCF7 WNT16  
CD44 FBXW11 FZD7 MET PPARD TCF7L1 WNT2B  


TruSeq Targeted RNA Expression Wnt Panel
RT-201-1004
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