Fast, simple mRNA library prep workflow for coding transcriptome analysis, combining cDNA synthesis and adapter tagging into a single step
Illumina Rapid mRNA Prep
Fast, simple mRNA library prep workflow for coding transcriptome analysis, combining cDNA synthesis and adapter tagging into a single step
Illumina Rapid mRNA Prep

Illumina Rapid mRNA Prep

Fast, simple mRNA library prep workflow for coding transcriptome analysis, combining cDNA synthesis and adapter tagging into a single step

3 hr

Assay 시간

< 1.5 hr

수작업 시간

1–1000 ng RNA

사용량

상세한 정보는 사양표를 확인하세요.

Overview

Illumina Rapid mRNA Prep offers the fastest and easiest mRNA-Seq library prep solution in the Illumina portfolio. A streamlined, 30-minute one-tube reaction combines cDNA synthesis and on-bead tagmentation. The rapid workflow supports low RNA sample inputs (1–1000 ng) and delivers high-accuracy nonstranded gene expression data.

Learn about on-bead tagmentation

Key features

  • Fast, simplified library prep

    The entire simplified workflow, including poly(A) capture and PCR steps, requires only 3 hours to generate a sequencing-ready mRNA library.

  • Optimized performance

    Provides excellent poly(A) capture efficiency and preserves library complexity, minimizing required sequencing depth for accurate, unbiased detection of the coding transcriptome.

  • High-quality data from low-input samples

    Reproducible results from as little as 1 ng of high-quality RNA* while achieving uniform coverage. 

    *Not compatible with formalin-fixed, paraffin-embedded (FFPE) samples.

Specifications

Assay time 3 hr
자동화 기능 리퀴드 핸들링 로봇
Description A fast and easy workflow for coding transcriptome analysis
Hands-on time < 1.5 hr
Input quantity 1–1000 ng RNA
기기 NextSeq 550 시스템, NextSeq 2000 시스템, NextSeq 1000 시스템, NextSeq 550Dx(Research Mode), NovaSeq X 시스템, NovaSeq 6000Dx(Research Mode), NovaSeq 6000 시스템, NovaSeq X Plus 시스템, MiSeq i100 시스템, MiSeq i100 Plus 시스템
방법 mRNA 시퀀싱
핵산 종류 RNA
특수 샘플의 종류 FFPE 호환 불가, 사용량이 적은 샘플
종 카테고리 포유류, 마우스, 인간, 래트
가닥 특이성 비가닥(Non-stranded)
기술 시퀀싱
변이 클래스 단일 염기 다형성(SNP), 유전자 융합, 새로운 전사물, 전사물 변이, 단일 염기서열 변이(SNV)

Required Products

The complete Illumina Rapid mRNA Prep workflow requires Rapid RNA library prep reagents, mRNA capture kit, and indexes (each sold separately):

  • Illumina Rapid RNA Prep—contains library prep reagents (96 sample or 16 sample kits available)
  • Illumina mRNA Capture Kit—contains reagents for capture of polyadenylated mRNA (96 sample or 16 sample kits available)
  • Illumina DNA/RNA UD Index Set, Tagmentation—each set of 96 indexes supports index labeling of 96 samples
/ 건

Applications

Illumina Rapid mRNA Prep enables preparation of sequencing-ready mRNA-Seq libraries for coding transcriptome analysis in 3 hr with less than 1.5 hr hands-on time.

Example workflow

1
Prep

Illumina Rapid mRNA Prep

2
Sequence
3
Analyze

Project recommendations

기기 권장 샘플 수 리드 길이
MiSeq i100 시스템, MiSeq i100 Plus 시스템

10–25M clusters per sample: 25M: 1–2, 50M: 1–5, 100M: 1–10

1 × 100 bp or 2 × 50 bp for gene expression, up to 2 × 100 bp for variant calling
NextSeq 1000 시스템, NextSeq 2000 시스템

10M clusters per sample: P1: 10, P2: 40, P3: 120, P4: 180

25M clusters per sample: P1: 4, P2: 16, P3: 48, P4: 72

1 × 100 bp or 2 × 50 bp for gene expression, up to 2 × 100 bp for variant calling
NovaSeq X 시스템, NovaSeq X Plus 시스템

10M clusters per sample: 1.5B: 150 (75/lane), 5B: 500 (62/lane), 10B: 1000 (125/lane), 25B: 2500 (312/lane)

25M clusters per sample: 1.5B: 60 (30/lane), 5B: 200 (25/lane), 10B: 400 (50/lane), 25B: 1000 (125/lane)

1 × 100 bp or 2 × 50 bp for gene expression, up to 2 × 100 bp for variant calling

Related applications and methods

mRNA Sequencing

NGS를 사용한 mRNA 시퀀싱(mRNA-Seq)은 기존의 알려진 또는 신규 전사물을 모두 식별하고 전사물 풍부도를 측정할 수 있습니다.

Documentation

제품 관련 자료
Support documentation

해당 제품 전용 지원 섹션은 현재 제공되지 않습니다

Compare

Illumina Rapid mRNA Prep
Assay time 3 hr
자동화 기능 리퀴드 핸들링 로봇
Description A fast and easy workflow for coding transcriptome analysis
Hands-on time < 1.5 hr
Input quantity 1–1000 ng RNA
기기 NextSeq 550 시스템, NextSeq 2000 시스템, NextSeq 1000 시스템, NextSeq 550Dx(Research Mode), NovaSeq X 시스템, NovaSeq 6000Dx(Research Mode), NovaSeq 6000 시스템, NovaSeq X Plus 시스템, MiSeq i100 시스템, MiSeq i100 Plus 시스템
방법 mRNA 시퀀싱
핵산 종류 RNA
특수 샘플의 종류 FFPE 호환 불가, 사용량이 적은 샘플
종 카테고리 포유류, 마우스, 인간, 래트
가닥 특이성 비가닥(Non-stranded)
기술 시퀀싱
변이 클래스 단일 염기 다형성(SNP), 유전자 융합, 새로운 전사물, 전사물 변이, 단일 염기서열 변이(SNV)

Selection tools:

Figures

High library complexity with Illumina Rapid mRNA Prep

High library complexity with Illumina Rapid mRNA Prep

Illumina Rapid mRNA Prep data from 1, 10, 25, and 100 ng of universal human reference (UHR) RNA shows the number of unique genes detected (≥ 5 mapped reads) across sequence depths downsampled to 1M–32M clusters (2M–64M paired-end reads).

High mapping efficiency with Illumina Rapid mRNA Prep

High mapping efficiency with Illumina Rapid mRNA Prep

Illumina Rapid mRNA Prep data shows high percentage of mapped reads across input amounts: 1, 10, 25, and 100 ng of universal human reference (UHR) RNA, downsampled to 20M clusters (40M paired-end reads).

Uniform coverage across transcripts with Illumina Rapid mRNA Prep

Uniform coverage across transcripts with Illumina Rapid mRNA Prep

Illumina Rapid mRNA Prep data shows consistent normalized coverage distributed along the length of transcripts across RNA input amounts: 1, 10, 25, and 100 ng of universal human reference (UHR) RNA, downsampled to 20M clusters (40M paired-end reads).

High reproducibility across high and low RNA inputs

High reproducibility across high and low RNA inputs

Illumina Rapid mRNA Prep shows high correlation of normalized gene counts between 1 ng and 100 ng input amounts (R2 = 0.99). Transcripts per million (TPM) generated by DRAGEN RNA v4.4.

Resources

Other resources

How on-bead tagmentation works

On-bead tagmentation library prep chemistry integrates DNA extraction, fragmentation, library prep, and library normalization.

라이브러리 프렙 (4)

Illumina Rapid RNA Prep, (S) Tagmentation (96 samples)

20158824

Includes reagents for preparing 96 samples of Rapid RNA libraries with Illumina Purification Beads. UD Indexes sold separately. Compatible with mRNA Capture kit for complete mRNA library prep workflow or RiboZero Plus kits for complete Total RNA library prep workflow or RNA Fast Hyb Enrichment kits for complete RNA Enrichment workflow, each sold separately.

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Illumina Rapid RNA Prep, (S) Tagmentation (16 samples)

20158823

Includes reagents for preparing 16 samples of Rapid RNA libraries with Illumina Purification Beads. UD Indexes sold separately. Compatible with mRNA Capture kit for Rapid mRNA library prep workflow or RiboZero Plus kits for Rapid Total RNA library prep workflow or Fast Hyb Enrichment kits for Rapid RNA Enrichment library prep workflow, each sold separately.

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Illumina mRNA Capture Kit (96 samples)

20158830

Includes reagents for capture and purification of polyadenylated mRNA for 96 samples. Compatible with Illumina Rapid RNA Prep, sold separately. for a complete Rapid mRNA library prep workflow.

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Illumina mRNA Capture Kit (16 samples)

20158829

Includes reagents for capture and purification of polyadenylated mRNA for 16 samples. Compatible with Illumina Rapid RNA Prep, sold separately. for a complete Rapid mRNA library prep workflow.

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인덱스 어댑터 (4)

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)에는 96개의 샘플을 라벨링하는 데 충분한 96개의 10bp 인덱스가 포함됩니다. 라이브러리 준비 및 인리치먼트 시약과 프로브 패널을 별도로 구매해 보세요.

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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)에는 96개 샘플을 라벨링하는 데 충분한 96개의 10bp 인덱스가 포함됩니다. 라이브러리 준비 및 인리치먼트 시약과 프로브 패널을 별도로 구매해 보세요.

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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)에는 96개 샘플을 라벨링하는 데 충분한 96개의 10bp 인덱스가 포함됩니다. 라이브러리 준비 및 인리치먼트 시약과 프로브 패널을 별도로 구매해 보세요.

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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)에는 96개 샘플을 라벨링하는 데 충분한 96개의 10bp 인덱스가 포함됩니다. 라이브러리 준비 및 인리치먼트 시약과 프로브 패널을 별도로 구매하세요.

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액세서리 제품 (3)

Illumina Purification Beads 30 ml

20119944

Illumina Purification Beads 30 ml

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Illumina Purification Bead, 100mL

20060057

라이브러리 클린업 및 크기 선발을 위한 비드 100 ml 컨테이너 1개가 포함됩니다. 라이브러리 준비 및 인리치먼트 시약, 프로브 패널 및 인덱스를 별도로 구매해 보세요.

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Illumina Purification Bead, 400mL

20060058

라이브러리 클린업 및 크기 선발을 위한 비드 100 ml 컨테이너 4개가 포함됩니다. 라이브러리 준비 및 인리치먼트 시약, 프로브 패널 및 인덱스를 별도로 구매해 보세요.

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FAQ

Step 1: Capture poly(A) RNA with the Illumina mRNA Capture Kit

Step 2: Illumina Rapid RNA Prep combines cDNA synthesis and on-bead tagmentation in a single-tube, 30-min reaction

Step 3: PCR adds index adapters

Step 4: Clean up with included Illumina Purification Beads

Illumina Rapid RNA Prep is available in 96-sample and 16-sample configurations. Illumina mRNA Capture is also available in 96-sample or 16-sample configurations.

Both methods start with oligo(dT) magnetic bead capture of poly(A) mRNA. Then Illumina Rapid mRNA Prep produces nonstranded libraries using tagmentation (3 hr total workflow time and < 1.5 hr hands-on time), while Illumina Stranded mRNA Prep produces stranded libraries using ligation (6.5 hr total workflow time and < 3 hr hands-on time).

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Ribo-Zero Plus Microbiome rRNA Depletion Kit

메타트랜스크립토믹스 연구를 위한 복잡한 미생물 샘플(예: 대변)에서 바람직하지 않은 숙주 및 전체 박테리아 리보솜 RNA의 빠르고 효율적인 제거.

TruSeq Stranded Total RNA

사람, 쥐, formalin-fixed paraffin-embedded (FFPE) 조직을 포함한 다양한 종(species)과 샘플 유형을 위한 강력하고 확장성이 뛰어난 전장 전사체(transcriptome) 분석(RNA-Seq) 솔루션.

Illumina DNA Prep

광범위한 시퀀싱 애플리케이션에서 사용할 라이브러리를 준비하기 위한 빠르고 통합된 워크플로우입니다.

Illumina DNA Prep with Enrichment

맞춤형 및 고정 인리치먼트 패널을 위한 빠른 통합 워크플로우 및 옵션을 갖춘 광범위한 애플리케이션을 위한 완벽한 표적 재시퀀싱 솔루션입니다.

Illumina Human Comprehensive Panel

Illumina Complete Long Read with Enrichment Library Prep Kit를 사용하여 단백질 코딩 유전자 내에 있는 매핑하기 까다로운 영역에 대해 향상된 커버리지를 제공하기 위해 사전 설계 및 최적화된 패널입니다. 필요한 ≥ 30× 표준 쇼트 리드 전장 유전체와 결합하여 분석됩니다.

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