DNaseI-SIM
DNaseI-SIM
This method is a simplified DNase I protocol specifically intended for plants. It contains an additional step of nuclei purification in Percoll gradients prior to DNase I digestion, in order to remove cellular debris and starch granules more efficiently. A DNA end-polishing step, using T4 DNA polymerase, is performed directly in the nuclei following DNase I digestion. These additional steps obviate the need for gel purification and its attendant loss of material.
Pros:
- Does not require gel purification
Cons:
- Optimized for plants only