EC-Seq
EC-Seq
Excision circle sequencing (EC-seq) allows the capture and analysis of immune locus rearrangements from whole thymic and splenic tissues.
RAG-mediated double-stranded cleavage at RSS sites precedes genomic deletion end processing by the nonhomologous end joining (NHEJ) complex. Coding ends are covalently hair-pinned and reopened prior to ligation. The excision circle junction is then ligated from unprocessed DNA ends with little modification.