Pol II CLIP
Pol II CLIP
Pol II CLIP was developed to isolate and sequence noncoding RNAs involved in transcriptional regulation, particularly RNAPII-mediated transcription. Briefly, cells are UV-crosslinked at 254 nm and lysed. Anti-RNAPII antibodies are used to immunoprecipitate the RNAPII complexes. Next, the RNAPII complexes are separated via proteinase K reverse-crosslinking. The released RNA released is isolated, reverse-transcribed, and prepared into sequencing libraries. The CLIP RNA samples are ready for sequencing.
Pros:
- Identifies ncRNA sequences involved in transcriptional regulation
- Simple procedure
Cons:
- None reported yet