RedBS-Seq/caMAB-Seq
RedBS-Seq/caMAB-Seq
redBS-Seq relies on the chemical reduction of f5C to hm5C by NaBH4. The hm5C is then detected in the same manner as m5C. In MAB-seq, 5fC is first reduced by NaBH4 to 5hmC. Owing to the combination of NaBH4 reduction with M.SssI treatment, 5caC is sequenced as T after bisulfite conversion, whereas C, 5mC, 5hmC and 5fC are read as C.
- RedBS-Seq: Booth M. J., Marsico G., Bachman M., Beraldi D. and Balasubramanian S. (2014) Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution. Nat Chem 6: 435-440
- caMAB-Seq: Wu H., Wu X. and Zhang Y. (2016) Base-resolution profiling of active DNA demethylation using MAB-seq and caMAB-seq. Nat Protoc 11: 1081-1100
- Van Haute L., Dietmann S., Kremer L., Hussain S., Pearce S. F., et al. (2016) Deficient methylation and formylation of mt-tRNA(Met) wobble cytosine in a patient carrying mutations in NSUN3. Nat Commun 7: 12039
- Wu H., Wu X. and Zhang Y. (2016) Base-resolution profiling of active DNA demethylation using MAB-seq and caMAB-seq. Nat Protoc 11: 1081-1100