RRMAB-Seq
RRMAB-Seq
RRMAB-seq is based on the pretreatment of genomic DNA with the enzyme MspI, which recognizes and cuts the CCGG consensus to enrich CpG-rich regions, like gene promoters. This is an adapted version of MAB-Seq.
Pros:
- Increased coverage on CpG-rich regions reduces the execution costs and increasing read depth.
Cons:
- Unable to distinguish 5fC/5caC from unmodified C within a non-CpG context