NextSeq System Sequencing Performance Parameters
| NextSeq 550 System High-Output Kit* | NextSeq 550 System Mid-Output Kit* | ||||
|---|---|---|---|---|---|
| Read Length | Total Time† | Output | Read Length | Total Time† | Output |
| 2 × 150 bp | 29 hrs | 100–120 Gb | 2 × 150 bp | 26 hrs | 32.5–39 Gb |
| 2 × 75 bp | 18 hrs | 50–60 Gb | 2 × 75 bp | 15 hrs | 16.25–19.5 Gb |
| 1 × 75 bp | 11 hrs | 25–30 Gb | |||
Reads Passing Filter
| NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit | |
|---|---|---|
| Single Reads | Up to 400 Million | Up to 130 Million |
| Paired-End Reads | Up to 800 Million | Up to 260 Million |
Quality Scores††
| NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit |
|---|---|
| > 75% bases higher than Q30 at 2 × 150 bp | > 75% bases higher than Q30 at 2 × 150 bp |
| > 80% bases higher than Q30 at 2 × 75 bp | > 80% bases higher than Q30 at 2 × 75 bp |
| > 80% bases higher than Q30 at 1 × 75 bp |
††A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
Estimated Sample Throughput for Key Applications
| NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit |
|---|---|
| 1 human whole genome | 3 exomes |
| 12 exomes | 12 enrichment panels |
| 16 transcriptomes | 96 amplicon panels |
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