TATL-Seq
TATL-Seq
TATL-Seq works in conjunction with TL-Seq to target the sequence around the 5'-UTRs of mRNA attached to polysomes. RNA from polysome gradient fractions is extracted, poly(A)-selected, and fragmented. Next, the TL-Seq protocol is followed to isolate and sequence the 5'-UTRs.
Pros:
- Sequences 5'-UTRs and identifies variants
- TATL-Seq enables de novo transcript leader annotation while simultaneously testing their translational activity in a single experiment
Cons:
- Selects short nucleotide fragments (50–80 nt)
- Labor-intensive; requires large amounts of starting material