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NovaSeq X 시리즈 사양

고처리량 시퀀싱의 새로운 기준

차세대 시퀀싱 데이터 생성량, 샘플 처리량, NovaSeq X 및 NovaSeq X Plus 시스템의 사양

NovaSeq X 시리즈 렌더링

플로우 셀당 필터 통과 리드

플로우 셀 유형 1.5B 10B 25B
싱글 엔드 리드 1.6B 개 10B 개 26B 개
페어드 엔드 리드 3.2B 개 20B 개 52B 개

품질 점수* 및 실행 시간†

플로우 셀 유형
 1.5B 10B 25B
Quality Score  
2 × 50 bp Q30을 넘는 염기의 ≥ 90%
2 × 100 bp Q30을 넘는 염기의 ≥ 85%
2 × 150 bp Q30을 넘는 염기의 ≥ 85%
런 타임  
2 × 50 bp 약 17시간 약 18시간 해당 없음
2 × 100 bp 약 20시간 약 22시간 해당 없음
2 × 150 bp 약 23시간 약 25시간 약 48시간

주요 어플리케이션*의 예상 샘플 처리량

플로우 셀 유형 1.5B 10B 25B
플로우 셀당 인간 게놈 약 4개 약 24개 약 64개
플로우 셀당 엑솜 약 41개 약 250개 약 750개
플로우 셀당 전사체 약 30개 약 200개 약 520개

Output per flow cell for various read lengthsa, b, c

NovaSeq X Series

Flow cell type 1.5B 10B 25B
2 × 50 bp ~165–238 Gb ~1–1.3 Tb ~2.6–3.5 Tb
2 × 100 bp ~330–476 Gb ~2–2.7 Tb ~5.3–7 Tb
2 × 150 bp 500–716 Gb ~3–4 Tb ~8–10.5 Tb
  1. Specifications based on Illumina PhiX control library or a TruSeq DNA Library created with NA12878 at supported cluster densities.
    NovaSeq X Plus system is capable of single flow cell runs or dual flow cell runs. Dual flow cell run delivers twice the output listed above.
    NovaSeq X system is capable of single flow cell runs.
  2. Performance at the higher range of output specification is not guaranteed. Actual output is dependent on library type, sample type, and run optimization.
  3. This kit requires system software v1.3 or newer.

Reads passing filter per flow cella

NovaSeq X Series

Flow cell type 1.5B 10B 25B
Single-end reads ~1.6–2.4 billion ~10–13 billion 26–35 billion
Paired-end reads 3.2–4.8 billion 20–26 billion ~52–70 billion
  1. Performance at the higher range of output specification is not guaranteed. Actual output is dependent on library type, sample type, and run optimization.

Quality scores and run timea, b

NovaSeq X Series

Flow Cell Type
 1.5B 10B 25B
Quality Scores  
2 × 50 bp ≥ 90% of bases higher than Q30
2 × 100 bp ≥ 85% of bases higher than Q30
2 × 150 bp ≥ 85% of bases higher than Q30
Run Time  
2 × 50 bp ~17 hr ~18 hr ~25 hr c
2 × 100 bp ~20 hr ~22 hr ~38 hr c
2 × 150 bp ~23 hr ~25 hr ~48 hr
  1. A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run. Quality scores are based on NovaSeq X series 10B reagents using an Illumina PhiX control library. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
  2. Run time includes automated cluster generation, sequencing, automated post-run wash, and base calling. Run times are based on Illumina PhiX control library at supported cluster densities. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
  3. This kit requires system software v1.3 or newer.

How to maximize performance on the NovaSeq X Series

Follow the best practices outlined in this technical note to assess library quality, optimize loading concentration, and pool libraries to maximize performance on the NovaSeq X Series.

Read technical note

Estimated sample throughput for key applicationsa

NovaSeq X Series

Flow cell type 1.5B 10B 25B
Human genomes per flow cell ~4 ~24 ~64
Exomes per flow cell ~41 ~250 ~750
Transcriptomes per flow cell ~30 ~200 ~520
  1. All sample throughputs are estimates per single flow cell run.
    Human genomes assumes > 120 Gb of data per sample to achieve 30× genome coverage.
    Exomes assumes ~8 Gb or ~40M reads per sample to achieve 100x coverage.
    Transcriptomes assumes ≥ 50M reads.
    Throughput may vary based on many factors including exome panel size, depth required, and library preparation kit used. Estimates are based on the lower range of output specification.

Instrument specifications

NovaSeq X specifications
검사실에서 보기
  • Laser wavelengths
    532 nm, 457 nm, 655 nm
  • Dimension with monitor
    W × D × H
    86.4 cm × 93.3 cm × 158.8 cm
    (34 in × 36.7 in × 62.5 in)
  • Dry weight of instrument
    (not including UPS; no core consumables)
    1171 lb
  • Dry weight of instrument
    (including UPS; no core consumables)
    1253 lb
  • Wet weight of instrument
    (not including UPS; loaded with core consumables)
    1296 lb
  • Crated weight of instrument
    1591 lb (722 kg)
  • Accessories pallet weight
    525 lb (238 kg)
View in-depth specs sheet (PDF)
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Innovation powers the NovaSeq X Series

Go on a virtual tour of the many innovations in the NovaSeq X Series and an overview of XLEAP-SBS chemistry. Learn about the integrated bioinformatics, reduced data footprint, lyophilized reagents, and other innovations.

Breakthrough technology innovations delivering a leap forward in sequencing