Illumina DNA Prep with Enrichment
맞춤형 및 고정 인리치먼트 패널을 위한 빠른 통합 워크플로우 및 옵션을 갖춘 광범위한 애플리케이션을 위한 완벽한 표적 재시퀀싱 솔루션입니다.
Targeted custom enrichment panels, providing simple and efficient solutions for multiple targeted sequencing applications.
Illumina Custom Enrichment Panel v2 provide fast, flexible content for targeted sequencing applications, enabling a fully customized enrichment solution.
| 설명 | 표적 시퀀싱 애플리케이션을 위한 속도, 유연성 및 확장성을 갖춘 완전 맞춤형 인리치먼트(enrichment) 패널입니다. |
|---|---|
| 기기 | MiSeq 시스템, MiSeqDx 기기, iSeq 100 시스템, NextSeq 550 시스템, NextSeq 2000 시스템, NextSeq 1000 시스템, MiniSeq 시스템, NextSeq 550Dx(Research Mode), NovaSeq X 시스템, NovaSeq 6000Dx(Research Mode), NextSeq 500 시스템, NovaSeq 6000 시스템, NovaSeq X Plus 시스템 |
| 라이브러리 준비 호환성 | 인리치먼트를 동반한 Illlumina 세포유리 DNA 프렙, Illumina Complete Long Reads with Enrichment, Human, Illumina DNA Prep with Enrichment |
| 방법 | 표적 인리치먼트 |
| 핵산 종류 | DNA |
| 종 카테고리 | 모든 종, 기타, 인간 |
| 기술 | 시퀀싱 |
Illumina Custom Enrichment Panel v2 is a 120 bp, double-stranded probe panel designed using DesignStudio and includes enrichment oligos for hybridization capture. Library prep, enrichment and index adapter reagents must be purchased separately.
Illumina Custom Enrichment Panel v2 is compatible with multiple targeted enrichment workflows. With DesignStudio, panels can be designed for use with multiple Illumina library prep kits.
표적 인리치먼트는 혼성화를 통해 관심 유전체 영역을 캡처하는 작업입니다. 표적 인리치먼트란 무엇이며, 여러 개의 샘플을 한꺼번에 연구하는 데 어떻게 활용할 수 있는지에 대해 알아보세요.
Illumina의 시퀀싱 및 마이크로어레이 기술은 DNA부터 RNA 분석, 후성유전학 등에 이르기까지 다양한 암 유전체학 연구 애플리케이션을 지원합니다.
다양한 유전적 복합 질환에 대한 연구를 가속화하기 위한 포괄적인 어레이 및 차세대 시퀀싱 솔루션입니다.
해당 제품 전용 지원 섹션은 현재 제공되지 않습니다
Custom panel sample calculations assume the indicated target coverage depths using 120 bp double-stranded DNA oligo panels (Illumina Custom Enrichment Panel v2) or 80-mer single-stranded dNA oligo panels (Illumina Custom Enrichment Panel) for target capture at 80% enrichment efficiency and 2 x 101 bp read length chemistry. Number of samples may vary depending on workflow handling, input sample or library quality, and actual sequencing output of each platform and flow cell.
| Part Number | Name | Volume (µl) | # of Samples: Illumina DNA Prep with Enrichment | # of Samples: Illumina Cell-Free DNA Prep with Enrichment | # of Samples: Illumina Complete Long Read Prep with Enrichment |
|---|---|---|---|---|---|
| 20073953 | Illumina Custom Enrichment Panel v2 (32 µl, 120 bp) | 32 µl | 96 | 8 (1-plex) or 32 (4-plex) |
64 |
| 20073952 | Illumina Custom Enrichment Panel v2 (384 µl, 120 bp) | 384 µl | 1152 | 96 (1-plex) or 384 (4-plex) |
768 |
| 20111339 | Illumina Custom Enrichment Panel v2 (1536 µl, 120 bp) | 1536 µl | 4608 | 384 (1-plex) or 1536 (4-plex) |
3072 |
| Panel volume per hyb | 4 ul probe panel per hyb rxn | 4 ul probe panel per hyb rxn | Dilute probe panel 5-fold, use 2.5 ul per hyb | ||
| Plexity | 12 samples per hyb* | 1 sample or 4 samples per hyb | 1 sample per hyb |
| Illumina Custom Enrichment Panel v2 | Illumina Custom Enrichment Panel | |
|---|---|---|
| Panel size, # of probes | 100 to 1,000,000 | 2000 to 67,000 |
| Oligo format | 120 bp double-stranded oligo probes | 80-mer single-stranded oligo probes |
| Available dispense sizes | 32 µl, 384 µl, 1536 µl | 80 µl |
| >99.9% of probes present, confirmed by NGS | Yes | No |
| Compatible Illumina library preps | Illumina DNA Prep with Enrichment, Illumina Cell-Free DNA Prep with Enrichment, Illumina Exome 2.5 Enrichment | Illumina DNA Prep with Enrichment, Illumina Cell-Free DNA Prep with Enrichment |
| Design tool | DesignStudio or Concierge Services | DesignStudio or Concierge Services |
| Turnaround time* | 3-6 weeks | 6-8 weeks |
| Panel cost | $ | $$$ |
*Additional ship time may be needed for non-US destinations
Library Prep and Array Kit Selector
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Comprehensive solution, from probe design to data analysis, for multiple targeted sequencing applications. Our custom-designed panels ensure high uniformity and coverage, with enhanced coverage of GC-rich and difficult to sequence areas.
Illlumina has supported protocols for use of Illumina Custom Enrichment v2 panels with Illumina DNA Prep with Enrichment, Illumina DNA Prep with Exome 2.5 Enrichment, and Illumina cfDNA Prep with Enrichment workflows.
| Illumina DNA Prep with Enrichment | Illumina DNA Prep with Exome 2.5 Enrichment | Illumina cfDNA Prep with Enrichment | |
|---|---|---|---|
| Minimum panel size | 500 probes | 100 probes (when combined with Exome 2.5) | 70kb or 700 probes, for double-stranded probes |
| Supported plexities | 1-plex or 12-plex | 12-plex | 1-plex or 4-plex |
| Volume of Panel used | 4 µl per enrichment reaction | 4 µl per enrichment reaction | 4 µl per enrichment reaction |
| # of samples supported (20073952 Illumina Custom Enrichment Panel v2 (32 µl, 120-bp) |
|
96 samples at 12-plex |
|
| # of samples supported (20073953 Illumina Custom Enrichment Panel v2 (384 µl, 120-bp) |
|
1,152 samples at 12-plex |
|
Illlumina has supported protocols for use of Illumina Custom Enrichment panels with Illumina DNA Prep with Enrichment and Illumina cfDNA Prep with Enrichment workflows.
The sample type compatibility is determined by the library prep kit paired with the custom panel. For Early Access we have supported protocols for use of the panels with Illumina DNA Prep with Enrichment (which is compatible with saliva, blood, purified gDNA and FFPE) and Illumina DNA Prep with Exome 2.5 Enrichment (saliva, blood and purified gDNA). Library prep and enrichment protocols with defined supported sample types will be released with launch of the Illumina cfDNA Prep with Enrichment kit.
The Illumina Concierge Design Team can help with complex designs, or designs for enrichment panels for non-human targets. This is a free service open to all customers. Contact your Illumina Sales Team representative to get connected to the Concierge team.
Illumina Custom Enrichment Panel V2 (32 µl, 120 bp)
20073953
Illumina Custom Enrichment Panel V2는 DesignStudio 또는 컨시어지 서비스를 사용하여 100개부터 최대 1,000,000개의 프로브까지 120 bp 이중 가닥 맞춤형 패널 설계를 지원하며 32 µl의 재료(Illumina DNA Prep with Enrichment와 페어링했을 때 8회의 인리치먼트 반응에 충분한 양)를 제공합니다. 라이브러리 준비, 인리치먼트 및 인덱스 어댑터 시약은 별도로 주문해야 합니다.
Illumina Custom Enrichment Panel V2 (384 µl, 120 bp)
20073952
Illumina Custom Enrichment Panel V2는 DesignStudio 또는 컨시어지 서비스를 사용하여 100개부터 최대 1,000,000개의 프로브까지 120 bp 이중 가닥 맞춤형 패널 설계를 지원하며 384 µl의 재료(Illumina DNA Prep with Enrichment와 페어링했을 때 96회의 인리치먼트 반응에 충분한 양)를 제공합니다. 라이브러리 준비, 인리치먼트 및 인덱스 어댑터 시약은 별도로 주문해야 합니다.
Illumina Custom Enrichment Panel V2 (1536 µl, 120 bp)
20111339
Illumina Custom Enrichment Panel V2는 DesignStudio 또는 컨시어지 서비스를 사용하여 100개부터 최대 1,000,000개의 프로브까지 120 bp 이중 가닥 맞춤형 패널 설계를 지원하며 1536 µl의 재료(Illumina DNA Prep with Enrichment와 페어링했을 때 384회의 인리치먼트 반응에 충분한 양)를 제공합니다. 라이브러리 준비, 인리치먼트 및 인덱스 어댑터 시약은 별도로 주문해야 합니다.
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