TruSight HLA v2 Sequencing Panel

Unambiguous, phase-resolved HLA typing in a single assay using proven Illumina NGS technology and Assign analysis software. Read More...
Select Product(s)

TruSight HLA v2 Sequencing Panel (24 samples)

20000215

Price
 
 

TruSight HLA v2 Sequencing Panel (24 samples, Automated)

20005170

Price
 
 
Accessory Products
What accessories do I need?

Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

Price
 
 

Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

Price
 
 

Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

Price
 
 

Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

Price
 
 

Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

Price
 
 

TruSeq Index Plate Fixture Kit

FC-130-1005

Price
 
 

Product Highlights

The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

  • High-resolution sequencing of 11 HLA loci
  • Workflow with library preparation, sequencing and data analysis/reporting
  • Accurate and high-resolution HLA typing for unambiguous results
Capture Full HLA Gene Sequences

TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis

The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexible options for post-analysis reporting of results.

High-Accuracy HLA Typing

The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works

TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights

The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications

Method-Specific Workflow Example

 

Supporting Data and Figures

 
TruSight HLA v2 Types 11 HLA Loci
Loci Target Sequence
HLA-A 4.1 kb (entire gene)
HLA-B 2.8 kb (exon 1 - intron 6)
HLA-C 4.2 kb (entire gene)
HLA-DRB1/3/4/5 4.6 - 5.1 kb (exon 2 - intron 4)
HLA-DQB1 6.9 kb (entire gene)
HLA-DPB1 9.7 kb (exon 2-3' UTR)
HLA-DQA1 7.3 kb (entire gene)
HLA-DPA1 10.3 kb (entire gene)

Manuals and Support Information

TruSight HLA v2 Documentation  

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSight HLA v2 Support 

With the introduction of the TruSight HLA v2 Sequencing Panel, which has been optimized to improve accuracy, increase efficiency, and reduce cost, the TruSight HLA v1 Sequencing Panel is being discontinued. Illumina remains committed to providing you with high-quality support and service during this transition. For v1 technical support information, visit the TruSight HLA v1 Support Page.

Related Products

Image Source
MiniSeq Reagent Kit

MiniSeq Reagent Kits bring the power of Illumina NGS to labs of all sizes. Access cost-efficient sequencing, even for low numbers of samples.


Image Source
MiSeq Reagent Kits v2

MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.


Image Source
NextSeq 500/550 v2 Kits

These sequencing reagent kits offer a simplified workflow and improved data quality compared to the prior v1 kit version.