Illumina has partnered with CareDx to distribute and support the TruSight HLA v2 Sequencing Panel and TruSight HLA Assign software. For TruSight HLA ordering and technical support, visit the CareDx TruSight HLA webpages.
The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:
TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.
The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexible options for post-analysis reporting of results.
The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.
TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.
The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.
The TruSight HLA v2 Sequencing Panel offers increased accuracy and efficiency in HLA typing compared to TruSight HLA v1, going from sample to report in less than 48 hours with 4 hours hands-on time.
| Loci | Target Sequence |
|---|---|
| HLA-A | 4.1 kb (entire gene) |
| HLA-B | 2.8 kb (exon 1 - intron 6) |
| HLA-C | 4.2 kb (entire gene) |
| HLA-DRB1/3/4/5 | 4.6 - 5.1 kb (exon 2 - intron 4) |
| HLA-DQB1 | 6.9 kb (entire gene) |
| HLA-DPB1 | 9.7 kb (exon 2-3' UTR) |
| HLA-DQA1 | 7.3 kb (entire gene) |
| HLA-DPA1 | 10.3 kb (entire gene) |
HLA Sequencing of IHWG Reference Samples with TruSight® HLA v2 Sequencing Panel
White Paper | PDF < 1 MB
HLA Sequencing of IHWG Reference Samples – Supplementary Tables
White Paper | EXCEL < 1 MB
TruSight Oncology Library Preparation and IDT xGen Exome Research Panel for Immuno-Oncology Research
Application Note | PDF
TruSight HLA v2 Sequencing Panel
Data Sheet | PDF < 1 MB
TruSight HLA v2 Sequencing Panel Reference Guide Documentation
Using a PhiX Control for TruSight HLA v2 Sequencing Runs Quick Reference Guide Documentation
TruSight HLA v2 Sequencing Panel Consumables and Equipment List Documentation
TruSight HLA v2 Sequencing Panel Checklist Documentation
Sequencing TruSight HLA v2 Libraries on a MiniSeq System Quick Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
With the introduction of the TruSight HLA v2 Sequencing Panel, which has been optimized to improve accuracy, increase efficiency, and reduce cost, the TruSight HLA v1 Sequencing Panel has been discontinued. Illumina remains committed to providing you with high-quality support and service during this transition.
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
MiniSeq Reagent Kits bring the power of Illumina NGS to labs of all sizes. Access cost-efficient sequencing, even for low numbers of samples.
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
These sequencing reagent kits offer increased stability and robustness over the v2 kit version.
