Nextera DNA Flex Library Prep Kit

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.Read More...
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Library Prep

Nextera DNA Flex Library Prep (24 Samples)

20018704

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Nextera DNA Flex Library Prep (96 Samples)

20018705

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Index Adapters

IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set B (96 Indexes, 96 Samples)

20027214

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

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Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)

20018707

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Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)

20018708

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Accessory Products
What accessories do I need?

Flex Lysis Reagent Kit (96 reactions)

20018706

Price
 
 

Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

Price
 
 

Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Price
 
 

NextSeq PhiX Control Kit

FC-110-3002

Price
 
 

Product Highlights

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

  • Fastest Illumina library prep workflow, with ~3.5 hours total time
  • Flexibility to accommodate variations in sample type, DNA input amount, and application
  • Optimized library prep performance, generating reliable results
Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.

Access Flexible Throughput Options

The IDT® for Illumina®-Nextera™ DNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell.  These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.

Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.

*Demonstrated protocols available.

 

Explore the Next Era in Library Prep

Discover the power that Nextera technology can bring to your lab through this interactive experience. Nextera library prep kit solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic
Introduction to Nextera DNA Flex Library Prep Kit

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (high output; based on 30× coverage of a human genome) Up to 2 × 150 bp
HiSeq 2500 System 2–10 samples per run  (dual flow cell; based on 30× coverage of a human genome) Up to 2 × 125 bp (rapid run) 
Up to 2 × 150 bp (high output)

Product Comparison

Nextera DNA Flex Library Prep Kit TruSeq DNA PCR-Free TruSeq DNA Nano
Assay Time ~3-4 hours (from DNA extraction to normalized library) 5 hours total assay time ~6 hours total assay time
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Description A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. A low-input research method that delivers high genome coverage quality and reduced bias.
Hands-On Time 1-1.5 hours 4 hours ~4 hours
Input Quantity Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). 1 ug DNA 100 ng genomic DNA
Mechanism of Action Bead-linked transposome Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free.
Method Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing
Multiplexing Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)
Specialized Sample Types Blood, Not FFPE-Compatible, Saliva Not FFPE-Compatible Low-Input Samples, Not FFPE-Compatible
Species Category Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish Human, Mammalian, Mouse, Other, Plant, Rat Human, Mammalian, Mouse, Other, Plant, Rat
Species Details Compatible with any species Compatible with most large DNA genomes. Compatible with most large DNA genomes.
Target Insert Size ~500 bp 350 bp or 550 bp 350 bp or 550 bp

Method-Specific Workflow Example

 

Supporting Data and Figures

How It Works

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