Illumina Sequencing Methods

Accurate total NGS solutions including library preparation, sequencing systems, and data analysis

Sequencing Methods

See What Sequencing Can Do For You

Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used.

Decade of Sequencing
One Decade of Sequencing

Explore the breakthroughs, advancements, and progress
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Key Sequencing Methods

DNA Sequencing

Discover the entire genome with whole-genome solutions, or focus on specific regions of interest with whole-exome and targeted sequencing options.

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RNA Sequencing

Take advantage of a broad range of sequencing techniques for targeted RNA or whole-transcriptome studies.

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Methylation Sequencing

Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.

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Sequencing Method Explorer

Use this interactive tool to explore experimental NGS library preparation methods compiled from the scientific literature.

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Sequencing Method Explorer

Sequencing Library Preparation

Library preparation kits are available for all sequencing methods, including whole-genome sequencing, whole-exome and targeted sequencing, RNA sequencing, methylation sequencing, and more. Kits accommodate a range of throughput needs, from manual protocols to fully automated workstations.

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Illumina NGS Methods Guide

All the information you need, from library preparation to sequencer selection to analysis. Select the best tools for your lab.

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Compare Sequencing Platforms
Find the Right Sequencing Platform

Compare sequencing systems and identify the best option for your lab and applications.

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NGS Introduction
Introduction to NGS

With its unprecedented throughput, scalability, and speed, NGS enables biological studies at a level never before possible.

Explore NGS
NextSeq 500
Improve Your Sequencing Results

NextSeq 500 v2 Kits offer better data quality, an improved signal to noise ratio, and a simpler workflow.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for common issues including overclustering, inconsistent quantitation, and sequencing through the insert.

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