16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. Both 16S and ITS rRNA gene sequencing are well-established methods for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult or impossible to study.
Unlike capillary sequencing or PCR-based approaches, next-generation sequencing (NGS) is a culture-free method that enables analysis of the entire microbial community within a sample. With the ability to combine many samples in a sequencing run, microbiology researchers can use NGS-based 16S and ITS rRNA sequencing as a cost-effective technique to identify strains that may not be found using traditional methods.
Illumina offers products to support 16S and ITS rRNA sequencing, from sample and library preparation to data analysis and interpretation. Our demonstrated workflow can help take the guess work out of your experiments. View our demonstrated sequencing protocols for16S rRNA and ITS rRNA.
Click on the below to view products for each workflow step.
Speed, accuracy and simplicity for far reaching applications in microbiology.iSeq 100 System
Affordable, fast, and accessible sequencing power for targeted or small genome sequencing in any lab.
BaseSpace Apps for taxonomic classification16S Metagenomics
Performs taxonomic classification of 16S rRNA targeted amplicon reads using an Illumina-curated version of the GreenGenes taxonomic database.Kraken Metagenomics
Assigns taxonomic labels to short DNA sequences with high sensitivity and speed using exact alignments of k-mers and a novel classification algorithm.ITS Metagenomics
Performs taxonomic classification of fungal rRNA targeted amplicon reads using the UNITE taxonomic database.
The Metagenomic Phylogenetic Analysis (MetaPhlAn) tool profiles microbial community composition from metagenomic shotgun sequencing data.QIIME Preprocessing
Quantitative Insights into Microbial Ecology (QIIME) is designed to take users from raw sequencing data to publication quality graphics and statistics.One Codex
Enables metagenomics analysis by rapidly assessing your samples from BaseSpace Sequence Hub with a fast, comprehensive, accurate data platform.