Illumina RNA Prep with Enrichment

Illumina RNA Prep with Enrichment uses On-Bead Tagmentation technology followed by simplified, single hybridization to provide an exceptionally fast workflow for RNA-based library preparation and enrichment.
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Rapid, Simple and Scalable Workflow

Prepare and enrich libraries in less than 9 hours total time with less than 2 hours of hands-on time. This workflow results in 50%+ time savings compared to TruSeq RNA Exome. Illumina Qualified Methods are available on a range of automation platforms through our partners.

High-quality Data from Difficult Samples

Get high sensitivity from as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples (such as FFPE).

Affordability and Focus

Isolating target transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth. Illumina RNA Prep with Enrichment is designed to provide exceptional capture efficiency and coverage uniformity, minimizing required sequencing depth for accurate detection without bias. Only 2.5 µL probes are needed per enrichment reaction.

Modular Panel Design for Flexibility

Choose from a variety of pre-built, validated probe pools or customize your own probes for maximum flexibility. Multiplex up to 384 samples in a single run with new unique dual index Sets C and D (coming soon). The modular design supports a wide range of RNA-Seq applications across regions of interest, enabling detection and discovery studies such as allele-specific expression, fusion detection, biomarker screening, and more.

RNA Exome Capabilities

Achieve rapid, targeted interrogation of an expansive number of target genes, including the RNA exome. Illumina RNA Prep with Enrichment can be used with the Illumina Exome Panel, which features a highly optimized probe set that delivers comprehensive coverage of coding RNA sequences. The Illumina Exome Panel includes > 425,000 probes, each constructed against the NCBI37/hg19 reference genome, covering > 98% of the RefSeq exome. The probe set captures > 214,000 targets, spanning 21,415 genes of interest. 

Respiratory Virus Detection

The Respiratory Virus Oligo Panel provides highly sensitive detection and characterization of common respiratory viruses, including coronavirus strains. The oligo probes used for hybrid–capture-based target enrichment remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving RNA viruses. Use the Respiratory Virus Oligo Panel with Illumina RNA Prep with Enrichment.