Products
 

We recommend ordering the new Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are obsolete and the last order date is January 30, 2025, as announced in PON2023-1440.

Illumina RNA Prep with Enrichment

Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity. Read More...
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Library Prep

Illumina® RNA Prep with Enrichment, (L) Tagmentation (16 Samples)

20040536

Price
 
 

Illumina® RNA Prep with Enrichment, (L) Tagmentation (96 Samples)

20040537

Price
 
 
Index Adapters

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Price
 
 

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Price
 
 

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Price
 
 

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20042667

Price
 
 
Panel

Illumina Exome Panel – Enrichment Oligos Only

20020183

Price
 
 

Respiratory Virus Oligos Panel V2

20044311

Price
 
 

Viral Surveillance Panel, RUO 96 Rxns

20088154

Price
 
 

Pan-Coronavirus Panel, RUO 96 Rxns

20088155

Price
 
 
Services

Illumina RNA Library Prep with Enrichment Training

20044763

Accessory Products

Illumina RNA Fast Hyb Enrichment (16 Samples)

20040540

Price
 
 

Illumina RNA Prep, (L) Tagmentation (16 Samples)

20040542

Price
 
 
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Product Highlights

Illumina RNA Prep with Enrichment uses On-Bead Tagmentation technology followed by simplified, single hybridization to provide an exceptionally fast workflow for RNA-based library preparation and enrichment.
Learn more about the technology

Rapid, Simple and Scalable Workflow

Prepare and enrich libraries in less than 9 hours total time with less than 2 hours of hands-on time. This workflow results in 50%+ time savings compared to TruSeq RNA Exome. Illumina Qualified Methods are available on a range of automation platforms through our partners.

High-quality Data from Difficult Samples

Get high sensitivity from as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples (such as FFPE).

Affordability and Focus

Isolating target transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth. Illumina RNA Prep with Enrichment is designed to provide exceptional capture efficiency and coverage uniformity, minimizing required sequencing depth for accurate detection without bias. Only 2.5 µL probes are needed per enrichment reaction.

Modular Panel Design for Flexibility

Choose from a variety of pre-built, validated probe pools or customize your own probes for maximum flexibility. Multiplex up to 384 samples in a single run. The modular design supports a wide range of RNA-Seq applications across regions of interest, enabling detection and discovery studies such as allele-specific expression, fusion detection, biomarker screening, and more.

RNA Exome Capabilities

Achieve rapid, targeted interrogation of an expansive number of target genes, including the RNA exome. Illumina RNA Prep with Enrichment can be used with the Illumina Exome Panel, which features a highly optimized probe set that delivers comprehensive coverage of coding RNA sequences. The Illumina Exome Panel includes > 425,000 probes, each constructed against the NCBI37/hg19 reference genome, covering > 98% of the RefSeq exome. The probe set captures > 214,000 targets, spanning 21,415 genes of interest. 

Respiratory Virus Detection

The Respiratory Virus Oligo Panel provides highly sensitive detection and characterization of common respiratory viruses, including coronavirus strains. The oligo probes used for hybrid–capture-based target enrichment remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving RNA viruses. Use the Respiratory Virus Oligo Panel with Illumina RNA Prep with Enrichment.

Learn More

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System Illumina RNA Prep with Enrichment + Exome Panel
Mid Output: 5
High Output: 16
(based on 25M reads per sample)		 2 × 100 bp
NextSeq 2000 System Illumina RNA Prep with Enrichment + Exome Panel
P2: 16
P3: 40
(based on 25M reads per sample)		 2 × 100 bp
NovaSeq 6000 System Illumina RNA Prep with Enrichment + Exome Panel
SP: 32
S1: 64
S2: 164
S4: 384
(based on 25M reads per sample)		 2 × 100 bp
MiSeq System Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 25 samples (based on 1M reads per sample)		 2 × 75 bp
MiniSeq System Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 25 samples (based on 1M reads per sample)		 2 × 75 bp
NextSeq 550 System Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 384 samples (based on 1M reads per sample)		 2 × 75 bp
MiSeq System Illumina RNA Prep with Enrichment + Viral Surveillance Panel or Pan-Coronavirus Panel
Up to 25 samples (based on 1M reads per sample)		 2 × 150 bp
NextSeq 550 System Illumina RNA Prep with Enrichment + Viral Surveillance Panel or Pan-Coronavirus Panel
Up to 384 samples (based on 1M reads per sample)		 2 × 150 bp

Product Comparison

Illumina RNA Prep with Enrichment TruSeq RNA Exome Illumina Stranded mRNA Prep
Assay Time < 9 hours ~2 Days 6.5 hours
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Content Specifications Captures the coding transcriptome when used with Illumina Exome Panel Captures the coding transcriptome/RNA exome Captures the coding transcriptome with strand information
Description A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement. A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information
Hands-On Time < 2 hours ~11 hours < 3 hours
Input Quantity 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples 25-1000 ng standard-quality total RNA
Mechanism of Action Bead-linked transposome Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails. PolyA capture, ligation-based addition of adapters and indexes
Method mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing Exome Sequencing, mRNA Sequencing mRNA Sequencing
Multiplexing Up to 384 Unique Dual Indexes (UDIs) Up to 24 single, 96 combinatorial (CD) dual Up to 384 Unique Dual Indexes (UDIs)
Technology Sequencing Sequencing Sequencing
Variant Class Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants

Method-Specific Workflow Example

 
Prepare Library for:
Targeted RNA Sequencing
 
Sequence Efficiently with:
NextSeq 550 System
NextSeq 2000 System
NovaSeq 6000 System
 
Analyze Your Data with:
DRAGEN RNA Pipeline
RNA-Seq Differential Expression
RNA-Seq Alignment App
DRAGEN RNA Pathogen Detection App
 

Supporting Data and Figures

 

Product Literature

Workflows for RNA sequencing

Brochure | PDF 1 MB

Illumina library preparation solutions

Brochure | PDF 2 MB

Detection and characterization of respiratory viruses, including SARS-CoV-2, using Illumina RNA Prep with Enrichment

Application Note | PDF < 1 MB

Improved detection of circulating transcripts

Application Note | PDF < 1 MB

NextSeq 1000 and NextSeq 2000 RNA sequencing solution

Application Note | PDF < 1 MB

Enrichment of coding transcripts in degraded samples using hybrid probe capture

Application Note | PDF < 1 MB

Surveillance of infectious disease through wastewater sequencing

Application Note | PDF < 1 MB

Faster detection of respiratory viruses using the MiniSeq Rapid Reagent Kit and Illumina RNA Prep with Enrichment

Application Note | PDF < 1 MB

NextSeq 550 RNA-Seq solution

Application Note | PDF < 1 MB

Rapid detection of respiratory pathogens using the MiniSeq System

Application Note | PDF < 1 MB

Data Sheet | PDF | 6 versions

Manuals and Support Information

Illumina RNA Prep with Enrichment (L) Tagmentation Documentation

Illumina RNA Prep with Enrichment (L) Tagmentation Reference Guide Documentation

Illumina RNA Prep with Enrichment (L) Tagmentation Checklist Documentation

Illumina RNA Prep with Enrichment (L) Tagmentation Consumables & Equipment Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All Illumina DNA RNA UD Indexes  Support   All Illumina RNA Prep with Enrichment (L) Tagmentation Support  

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Not for use in diagnostic procedures (except as specifically noted).

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