We recommend ordering the new Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are obsolete and the last order date is January 30, 2025, as announced in PON2023-1440.
Illumina RNA Library Prep with Enrichment Training
20044763
Illumina RNA Prep with Enrichment uses On-Bead Tagmentation technology followed by simplified, single hybridization to provide an exceptionally fast workflow for RNA-based library preparation and enrichment.
Learn more about the technology
Prepare and enrich libraries in less than 9 hours total time with less than 2 hours of hands-on time. This workflow results in 50%+ time savings compared to TruSeq RNA Exome. Illumina Qualified Methods are available on a range of automation platforms through our partners.
Get high sensitivity from as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples (such as FFPE).
Isolating target transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth. Illumina RNA Prep with Enrichment is designed to provide exceptional capture efficiency and coverage uniformity, minimizing required sequencing depth for accurate detection without bias. Only 2.5 µL probes are needed per enrichment reaction.
Choose from a variety of pre-built, validated probe pools or customize your own probes for maximum flexibility. Multiplex up to 384 samples in a single run. The modular design supports a wide range of RNA-Seq applications across regions of interest, enabling detection and discovery studies such as allele-specific expression, fusion detection, biomarker screening, and more.
Achieve rapid, targeted interrogation of an expansive number of target genes, including the RNA exome. Illumina RNA Prep with Enrichment can be used with the Illumina Exome Panel, which features a highly optimized probe set that delivers comprehensive coverage of coding RNA sequences. The Illumina Exome Panel includes > 425,000 probes, each constructed against the NCBI37/hg19 reference genome, covering > 98% of the RefSeq exome. The probe set captures > 214,000 targets, spanning 21,415 genes of interest.
The Respiratory Virus Oligo Panel provides highly sensitive detection and characterization of common respiratory viruses, including coronavirus strains. The oligo probes used for hybrid–capture-based target enrichment remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving RNA viruses. Use the Respiratory Virus Oligo Panel with Illumina RNA Prep with Enrichment.
Learn MoreInstrument | Recommended Number of Samples | Read Length |
---|---|---|
NextSeq 550 System | Illumina RNA Prep with Enrichment + Exome Panel Mid Output: 5 High Output: 16 (based on 25M reads per sample) |
2 × 100 bp |
NextSeq 2000 System | Illumina RNA Prep with Enrichment + Exome Panel P2: 16 P3: 40 (based on 25M reads per sample) |
2 × 100 bp |
NovaSeq 6000 System | Illumina RNA Prep with Enrichment + Exome Panel SP: 32 S1: 64 S2: 164 S4: 384 (based on 25M reads per sample) |
2 × 100 bp |
MiSeq System | Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel Up to 25 samples (based on 1M reads per sample) |
2 × 75 bp |
MiniSeq System | Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel Up to 25 samples (based on 1M reads per sample) |
2 × 75 bp |
NextSeq 550 System | Illumina RNA Prep with Enrichment + Respiratory Virus Oligo Panel Up to 384 samples (based on 1M reads per sample) |
2 × 75 bp |
MiSeq System | Illumina RNA Prep with Enrichment + Viral Surveillance Panel or Pan-Coronavirus Panel Up to 25 samples (based on 1M reads per sample) |
2 × 150 bp |
NextSeq 550 System | Illumina RNA Prep with Enrichment + Viral Surveillance Panel or Pan-Coronavirus Panel Up to 384 samples (based on 1M reads per sample) |
2 × 150 bp |
Illumina RNA Prep with Enrichment | TruSeq RNA Exome | Illumina Stranded mRNA Prep | |
---|---|---|---|
Assay Time | < 9 hours | ~2 Days | 6.5 hours |
Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
Content Specifications | Captures the coding transcriptome when used with Illumina Exome Panel | Captures the coding transcriptome/RNA exome | Captures the coding transcriptome with strand information |
Description | A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples | Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement. | A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information |
Hands-On Time | < 2 hours | ~11 hours | < 3 hours |
Input Quantity | 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples | 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples | 25-1000 ng standard-quality total RNA |
Mechanism of Action | Bead-linked transposome | Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails. | PolyA capture, ligation-based addition of adapters and indexes |
Method | mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing | Exome Sequencing, mRNA Sequencing | mRNA Sequencing |
Multiplexing | Up to 384 Unique Dual Indexes (UDIs) | Up to 24 single, 96 combinatorial (CD) dual | Up to 384 Unique Dual Indexes (UDIs) |
Technology | Sequencing | Sequencing | Sequencing |
Variant Class | Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants | Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants | Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants |
Brochure | PDF 1 MB
Illumina library preparation solutions
Brochure | PDF 2 MB
Application Note | PDF < 1 MB
Improved detection of circulating transcripts
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 RNA sequencing solution
Application Note | PDF < 1 MB
Enrichment of coding transcripts in degraded samples using hybrid probe capture
Application Note | PDF < 1 MB
Surveillance of infectious disease through wastewater sequencing
Application Note | PDF < 1 MB
Application Note | PDF < 1 MB
Application Note | PDF < 1 MB
Rapid detection of respiratory pathogens using the MiniSeq System
Application Note | PDF < 1 MB
Data Sheet | PDF | 6 versions
Illumina RNA Prep with Enrichment (L) Tagmentation Documentation
Illumina RNA Prep with Enrichment (L) Tagmentation Reference Guide Documentation
Illumina RNA Prep with Enrichment (L) Tagmentation Checklist Documentation
Illumina RNA Prep with Enrichment (L) Tagmentation Consumables & Equipment Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All Illumina DNA RNA UD Indexes Support All Illumina RNA Prep with Enrichment (L) Tagmentation Support