Understanding the genetic code

NGS technology enables massively parallel DNA analysis for a deeper understanding of biology

DNA Sequencing

Illumina next-generation sequencing (NGS) technology uses clonal amplification and sequencing by synthesis (SBS) chemistry to enable rapid, accurate sequencing. The process simultaneously identifies DNA bases while incorporating them into a nucleic acid chain. Each base emits a unique fluorescent signal as it is added to the growing strand, which is used to determine the order of the DNA sequence.

NGS technology can be used to sequence the DNA from any organism, providing valuable information in response to almost any biological question. A highly scalable technology, DNA sequencing can be applied to small, targeted regions or the entire genome through a variety of methods, enabling researchers to investigate and better understand health and disease.

Introduction to DNA Sequencing
  • Sequences large stretches of DNA in a massively parallel fashion, offering advantages in throughput and scale compared to capillary electrophoresis–based Sanger sequencing
  • Provides high resolution to obtain a base-by-base view of a gene, exome, or genome
  • Delivers quantitative measurements based on signal intensity
  • Detects virtually all types of genomic alterations, including single nucleotide variants, insertions and deletions, copy number changes, and chromosomal aberrations
  • Offers high throughput and flexibility to scale studies and sequence multiple samples simultaneously
Illumina NGS Methods Guide

All the information you need, from library preparation to sequencer selection to analysis. Select the best tools for your lab.

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Find the Right DNA Sequencing Library Prep Kit

Determine the best kit for your needs based on your method of interest. Access detailed product information, including comparison tables and specifications.

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Kit Selector
Whole-Genome Sequencing

Whole-genome sequencing is the most comprehensive method for analyzing the genome. Rapidly dropping sequencing costs and the ability to obtain valuable information about the entire genetic code make whole-genome sequencing a powerful research tool.

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Targeted Resequencing

With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced, allowing researchers to focus time, expenses, and analysis on specific areas of interest.

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ChIP Sequencing

By combining chromatin immunoprecipitation (ChIP) assays and sequencing, ChIP sequencing (ChIP-Seq) is a powerful method to identify genome-wide DNA binding sites for transcription factors and other proteins.

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Cancer DNA Sequencing
Cancer DNA Sequencing

NGS-based cancer DNA sequencing methods enable researchers to detect rare somatic variants, tumor subclones, and circulating DNA fragments. Learn more about cancer sequencing methods.

Genotyping Solutions
Samples for Genotyping

Sequencing- and array-based genotyping technologies can provide insight into the functional consequences of genetic variation. Learn more about genotyping.

Causal Variant Discovery
Causal Variant Discovery

High-throughput DNA sequencing technologies allow researchers to rapidly screen large sample numbers to find causal variants associated with complex diseases. Learn more about causal variant discovery.

Microbial Sequencing
Microbial Sequencing

Analysis of microbial species using DNA sequencing can inform environmental metagenomics studies, infectious disease surveillance, molecular epidemiology, and more. Learn more about microbial sequencing methods.

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Next-Generation Sequencing Technology
NGS Technology

NGS has become an everyday research tool used to address today’s complex biological questions.

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Desktop DNA Sequencer
Benchtop DNA Sequencer

Bring genome, exome, and transcriptome sequencing to your benchtop with the powerful NextSeq Series.

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Sequencing Data Analysis
DNA Sequencing Data Analysis

Find intuitive analysis tools that transform raw DNA sequencing data into meaningful results.

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Sequencing Platform Comparison Tool
Find the Right Sequencing Platform

Compare the speed and throughput of Illumina sequencing systems to find the best option for your lab.

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Gene Panel Finder
Gene Panel Finder

Identify sequencing panels that target your genes of interest.

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Sequencing Troubleshooting Tips
Sequencing Troubleshooting Tips

These short videos provide expert tips for issues such as overclustering, inconsistent quantitation, and sequencing through the insert.

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