Survey the genomes of entire communities

Better understand the role of microbes in human health and disease

Human Microbiome Analysis

Human microbiome analysis is the study of microbial communities found in and on the human body. The goal of human microbiome studies is to understand the role of microbes in health and disease.

Traditionally, studying samples from human skin, stool, or blood relied on time- and labor-intensive microbiology techniques of growing and isolating individual organisms followed by phenotypic or genotypic analysis. Microbial community profiling within a single sample was not possible with these methods.

Introduction to Human Microbiome Analysis

The advent of next-generation sequencing (NGS) enabled several high-profile collaborative projects including the Human Microbiome Project and MetaHIT, which have published a wide range of data on the human microbiome using NGS as a foundational tool.

The throughput and cost savings of NGS have fueled metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.

Experimental NGS methods for analyzing the human microbiome include:
Shotgun metagenomic sequencing
  • A DNA sequencing method that enables comprehensive sampling of all genes in all organisms in a given complex microbial sample.
16S rRNA sequencing
  • A 16S ribosomal RNA gene sequencing method used to identify and compare bacteria present within a given sample.
Microbial metatranscriptomics
  • Analysis of all RNAs encoded by a group of microorganisms within a complex sample.
Gene-Level Metagenomics for Microbiome Research
Metagenomics Data Sets

This webcast will introduce a novel gene-level bioinformatics approach for identifying microbial species and strains associated with human disease from metagenomic sequencing data sets. Samuel Minot, PhD will also demonstrate an interactive microbiome discovery portal.

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Microbiome Sequencing in High-host and
Low-biomass Samples
Microbiome Sequencing

This webcast will discuss new sequencing methods, including those generating synthetic long reads, with a focus on their relevance for microbial community analysis. Dr. Stefan J. Green will address practical approaches to high host and low microbial biomass issues.

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Microbial Transcriptomics with rRNA Depletion

The Ribo-Zero Plus rRNA Depletion Kit removes rRNA before RNA-Seq, enabling you to focus on high-value portions of the transcriptome. This technical note demonstrates rRNA depletion performance on microbial isolate and community samples, while comparing against previous kit versions.

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Microbial Transcriptomics with rRNA Depletion

Human Microbiome Research Stories

 
The Time is Now for Microbiome Studies
The Time is Now for Microbiome Studies

Whole-genome shotgun sequencing and transcriptomics provide researchers and pharmaceutical companies with data to refine drug discovery and development.

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Sequencing a Mouthful of Microbes
Sequencing a Mouthful of Microbes

The MiSeq System is enabling the Saca la Lengua project research team to sequence thousands of samples to identify the variety of bacteria and fungi that live in the human mouth.

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NGS is Revealing the Mysterious World of Microbes
NGS is Revealing the Mysterious World of Microbes

Researchers at Microba are investigating the genomes of microbes to improve our understanding of human health, disease, and microbial evolution.

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Learn how to design microbiome studies that maximize power and chances of success within a given research budget, and better understand how personalized diet-microbiome interactions could be affecting outcomes of your studies.

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A Guide to Diet-Microbiome Study Design
Explore the NextSeq 2000

The NextSeq 2000 empowers sequencing for high-throughput applications. With over 75 updates, this system offers dry instrumentation, easier run setup, and fast second run analysis with DRAGEN software onboard. Experience our simplest workflows yet, and explore the possibilities that exome sequencing, target enrichment, single-cell profiling, and transcriptome sequencing enable.

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Related Human Microbiome Research

Cancer Microbiome Research
Cancer Microbiome Research

Microbial communities can influence host immune response, affecting cancer progression and treatment efficacy. As NGS-based research continues to explore host–microbiome interactions, there is hope that information about the human microbiome might inform immunotherapeutic strategies.

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How Microbiome Multi-Omics Can Bolster Human Health
How Microbiome Multi-Omics Can Bolster Human Health

Sequencing technologies are enabling a deeper analysis of the gut's microbiome. Researchers can now explore what our microbial inhabitants are doing and how they contribute to, or protect from, disease.

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MiSeq, 16S rRNA Sequencing and the American Gut Project
MiSeq, 16S rRNA Sequencing and the American Gut Project
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Composition and Dynamics of the Human Gut Virome<
Composition and Dynamics of the Human Gut Virome
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Microbial Sequencing for Microbiome Studies
Microbial Sequencing for Microbiome Studies

See how Illumina NGS is being used to sequence complex microbial populations.

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Metagenomics for Virus Discovery on Skin
Metagenomics for Virus Discovery on Skin

Illumina sequencers offer deep coverage to identify novel HPV types correlated with non-melanoma skin cancers.

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