NovaSeq 6000 Sequencing System

Join other NovaSeq owners in the NovaSeq Online Community. Collaborate with Illumina moderators and NovaSeq owners. Discuss best practices, troubleshoot, and learn about how others are using NovaSeq preparation kits, push-button sequencing, and automated data analysis to fuel their research.
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4800-6000 Gb

output range

32-40 B

reads per run

2 × 250 bp

max read length

View All NovaSeq Specifications

*Specifications based on a dual flow cell run of S4 flow cells.

Sequencing Complex Plant Genomes at Texas A&M AgriLife

Charlie Johnson, Director of Genomics and Bioinformatics at Texas A&M AgriLife, discusses the work his lab is doing with complex plant and animal genomes and how the NovaSeq 6000 System makes it all possible.

Chan Zuckerberg Biohub: Conducting Innovative Experiments with NGS

Steve Quake and Joe DeRisi discuss their mission to conduct innovative experiments and new kinds of collaborations in genomics. Their recent partnering with Stanford, UCSF and Berkeley enables research that may improve human health.

“This is the most exciting time to be in genomics,” says Charlie Johnson from Texas A&M University. He, along with Chris Austin and Larry Croft from Deakin University in Australia and Ryo Koyanagi from the Okinawa Institute of Science and Technology (OIST) in Japan, discuss how the NovaSeq System’s high throughput and cost effectiveness are making large-scale projects possible across the globe.

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One Decade of Sequencing

Explore the breakthroughs, advancements, and progress

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Featured Products

NovaSeq Reagent Kits
NovaSeq Reagent Kits

Reagent kits for the NovaSeq 6000 System provide ready-to-use cartridge-based reagents for cluster generation and SBS.

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NovaSeq Xp Workflow
NovaSeq Xp Workflow

The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 System flow cell, without additional instrumentation.

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TruSeq Stranded Total RNA
TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.

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Nextera XT DNA Library Preparation Kit
Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement

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