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NovaSeq Series

Hear from the Creators
Hear from the Creators

Discover the inspiration behind the NovaSeq Series and see how we’re revolutionizing genomics.
NovaSeq Series Webinar
NovaSeq Series Webinar

Register to get more details about the capabilities of the new NovaSeq Series.
NovaSeq Series Specification Sheet
NovaSeq Series Specification Sheet

Complete projects faster and more economically than ever before—and redefine the limits of your research.

Scalable Throughput

To meet different user needs, the NovaSeq Series is offered in 2 configurations. The NovaSeq 5000 System offers cost-effective high-throughput sequencing across a broad range of applications. The NovaSeq 6000 System delivers those applications with unprecedented throughput at the lowest cost per sample. No matter which system you choose, you’ll have throughput that matches your science.

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Next: Highly Flexible

Highly Flexible

The NovaSeq Series provides tremendous scalability and flexibility to accommodate diverse applications and project sizes, while achieving maximum operational efficiency. Users can run 1 or 2 flow cells at a time and choose between 4 flow cell types, easily adjusting output for each sequencing run. With highly tunable data output you can run virtually any genome, sequencing method, and scale of project.

Next: Broad Range of Applications

Broad Range of Applications

The NovaSeq Series is compatible with a broad range of Illumina library preparation kits, from transcriptome sequencing to whole-genome sequencing and everything in between. High-quality, high-coverage sequencing offers a comprehensive view of the genome to detect variants accurately, characterize isoforms, and more. With increased throughput and reduced price per sample, the NovaSeq Series is ideal for data-intensive applications.

Explore Applications

Next: Streamlined Operation

Streamlined Operation

The NovaSeq Series combines the features that make our benchtop sequencers so easy to use with the power of a high-throughput sequencing platform. It offers fully automated, onboard cluster generation to minimize hands-on time. Integrated, ready-to-use reagent cartridges streamline run setup and prevent improper loading. RFID encoded consumables enable fully automated reagent traceability. In all, it takes only minutes to set up a run on the NovaSeq 5000 System or NovaSeq 6000 System, giving you increased lab efficiency.

Next: Scalable Throughput
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Workflow

 
Manage Workflow

Manage the workflow seamlessly with BaseSpace Clarity LIMS or your existing LIMS system.

 
Prepare Libraries

Prepare libraries with a broad range of high performance library preparation kits from Illumina.

 
Generate Sequences

Generate sequences with proven sequencing by synthesis (SBS) chemistry more than a decade in the making.

 
Analyze Data

Analyze data with seamless integration into BaseSpace Sequence Hub. Share your results in a secure cloud environment.

 
Interpret Results

Interpret results, get insights, and drive discovery with BaseSpace Variant Interpreter (Beta) and BaseSpace Cohort Analyzer.

See NovaSeq Application-Specific Workflows

System Specifications*

167 Gb–6 Tb

output range

1.6–20 billion

reads per run

2 × 150 bp

max read length

View All NovaSeq Specifications

*Specifications based on a dual flow cell run of S4 flow cells which have not been released, therefore performance metrics are subject to change.

Customer Stories

Regeneron: Scaling NGS to Deliver More Discoveries, Faster

Aris Baras and John Overton of Regeneron discuss their latest work and overall mission to explore new variations in genomes that can help explain disease. Hear how they plan to utilize the NovaSeq 6000 System.

Chan Zuckerbeg Biohub: Conducting Innovative Experiments with NGS

Steve Quake and Joe DeRisi discuss their mission to conduct innovative experiments and new kinds of collaborations in genomics. Their recent partnering with Stanford, UCSF and Berkeley enables research that may improve human health.

Decade of Sequencing Graphic

One Decade of Sequencing

Explore the breakthroughs, advancements, and progress

Learn More
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Featured Products

NovaSeq Reagent Kits
NovaSeq Reagent Kits

Reagent kits for the NovaSeq 5000 and NovaSeq 6000 Systems provide ready-to-use cartridge-based reagents for cluster generation and SBS.

TruSeq DNA PCR-Free Library Preparation Kit
TruSeq DNA PCR-Free Library Preparation Kit

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.

TruSeq Stranded Total RNA Library Prep Kit
TruSeq Stranded Total RNA Library Prep Kit

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and FFPE.

View All NovaSeq Products

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