AmpliSeq for Illumina Cancer Hotspot Panel v2

Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.Read More...
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Panel

AmpliSeq™ Cancer HotSpot Panel v2 for Illumina®

20019161

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)

20019105

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Accessory Products

AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:

Relevant Gene Content
  • Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples
Accurate Data
  • Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis

The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
iSeq 100 System 16 samples per run (assumes minimum coverage of 500×) 2 × 150 bp
MiniSeq System Samples per run: mid output: 32, high output: 96 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run (by reagent kit version): v2 nano: 4, v2 micro: 16, v2: 60, v3: 96 (assumes minimum coverage of 500×) 2 × 150 bp

Product Comparison

AmpliSeq for Illumina Cancer Hotspot Panel v2 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3 TruSight Tumor 170 TruSight Tumor 15
Assay Time 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) ~2 days (Library Prep) 7 hours
Cancer Type Pan-Cancer, Solid Tumor Solid Tumor Solid Tumor Solid Tumor Solid Tumor
Content Specifications Hotspot regions of 50 genes with known associations to cancer DNA and RNA targets for 52 oncogenes DNA and RNA targets for 161 oncogenes Probes enrich for full coding sequences of 170 genes. Calls single nucleotide variants, small insertions, and deletions in 151 genes, amplifications in 59 genes, and fusions plus splice variants in 55 genes. Amplifies 250 amplicons from 15 genes associated with solid tumors
Description Somatic analysis research into hotspot regions of 50 cancer-related genes. Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. Perform comprehensive somatic variant detection research in solid tumors using variant calling information from both DNA and RNA. Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow.
Input Quantity 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 40 ng DNA and/or RNA 20 ng
Method Amplicon Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Target Enrichment , Target Enrichment, Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing 96 dual index combinations 96 dual index combinations 96 dual index combinations Up to 32-plex for DNA using both index sets, up to 16-plex for RNA 1-24-plex
Nucleic Acid Type DNA DNA, RNA DNA, RNA RNA, DNA DNA
Specialized Sample Types Blood, FFPE Tissue Blood, FFPE Tissue Blood, FFPE Tissue FFPE Tissue, Low-Input Samples FFPE Tissue, Low-Input Samples
Species Category Human Human Human Human Human

Method-Specific Workflow Example

 

Related Products

AmpliSeq for Illumina Focus Panel

Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.


TruSight Tumor 15

Focused sequencing panel to assess 15 genes that are commonly mutated in solid tumors in a single assay, with a simple, rapid workflow.


AmpliSeq for Illumina Custom DNA Panel

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.


References
  1. Kingsmore S. Catalogue of Somatic Mutations in Cancer – Home Page. http://cancer.sanger.ac.uk/cosmic. Accessed October 25, 2017.