A key element of Illumina next-generation sequencing (NGS) is high-quality library preparation. Illumina library prep protocols can accommodate a range of throughput needs, from lower-throughput protocols for small laboratories to fully automated library preparation workstations for large laboratories or genome centers.
Illumina offers comprehensive sequencing library preparation solutions for a wide variety of NGS methods, including whole-genome sequencing, targeted DNA sequencing, whole-transcriptome sequencing, targeted RNA sequencing, and more. These solutions support a variety of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.
Illumina offers an extensive assortment of easy-to-use next-gen sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. To find the right kit for your needs, use the selection tool below, or view a filterable list of library prep kits.
The NeoPrep Library Prep System radically simplifies preparation of next-generation sequencing libraries by significantly decreasing the number of steps, improving reproducibility, and greatly reducing hands-on time. The system performs PCR amplification, quantification, and normalization, generating up to 16 sequencing-ready libraries per run.View NeoPrep System
For labs preparing large quantities of NGS libraries, liquid-handling robots and additional automation solutions provide high-throughput capacity and important quality control touch points. Visit the page below to learn more about Illumina-qualified sequencing library prep automation methods available through our partners.See Automation Options
Access a summary of NGS methods compiled from peer-reviewed publications. Explore the wide range of questions that can be addressed with Illumina technology. Find method descriptions, pros and cons, and more.Find Methods