Comprehensive NGS library preparation solutions

NGS Library Preparation

A key element of Illumina next-generation sequencing (NGS) is high-quality library preparation. Illumina library prep protocols can accommodate a range of throughput needs, from lower-throughput protocols for small laboratories to fully automated library preparation workstations for large laboratories or genome centers.

Illumina offers comprehensive sequencing library preparation solutions for a wide variety of NGS methods, including whole-genome sequencing, targeted DNA sequencing, whole-transcriptome sequencing, targeted RNA sequencing, and more. These solutions support a variety of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.

Illumina offers an extensive assortment of easy-to-use next-gen sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. To find the right kit for your needs, use the selection tool below, or view a filterable list of library prep kits.

Library Prep Kit Selector Library Prep Kit Selector
Kits for Preparing NGS Libraries

The NeoPrep Library Prep System radically simplifies preparation of next-generation sequencing libraries by significantly decreasing the number of steps, improving reproducibility, and greatly reducing hands-on time. The system performs PCR amplification, quantification, and normalization, generating up to 16 sequencing-ready libraries per run.

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Automated Library Prep Buyer's Guide

Learn what to consider when evaluating NGS library prep automation systems.

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For labs preparing large quantities of NGS libraries, liquid-handling robots and additional automation solutions provide high-throughput capacity and important quality control touch points. Visit the page below to learn more about Illumina-qualified sequencing library prep automation methods available through our partners.

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Access a summary of NGS methods compiled from peer-reviewed publications. Explore the wide range of questions that can be addressed with Illumina technology. Find method descriptions, pros and cons, and more.

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Library Prep with Digital Microfluidics
Library Prep with Digital Microfluidics

See how digital microfluidics streamlines NGS experiments by enabling library preparation automation.

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New to NGS?
New to NGS?

Find out how NGS technology works and see the broad range of experiments you can perform.

Explore NGS
Library Prep and NGS Innovation
Library Prep and NGS Innovation

See how advances in library preparation technology will further the promise of NGS.

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Push-Button Informatics
Push-Button Informatics

Access user-friendly genomic data analysis tools that can be used by any researcher, regardless of bioinformatics experience.

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Sequencing Method Overviews
Sequencing Solutions

Learn about a wide variety of DNA, RNA, and epigenetic sequencing methods. Find complete solutions, from library preparation to data analysis.

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NextSeq 500 Desktop Sequencer
Speed and Simplicity for Everyday Genomics

The NextSeq Series desktop sequencer offers the perfect combination of high-throughput sequencing, flexibility, and accuracy.

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