TruSeq Methyl Capture EPIC Library Prep Kit

This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.Read More...
Select Product(s)

TruSeq-Methyl Capture EPIC Library Prep Kit (12 samples, 4 indexes)

FC-151-1002

Price
 
 

TruSeq-Methyl Capture EPIC Library Prep Kit (48 samples, 12 indexes)

FC-151-1003

Price
 
 

Product Highlights

Targeted methylation sequencing (Methyl-Seq) offers a balanced, cost-effective choice between whole-genome bisulfite sequencing and methylation arrays that can support both screening and biomarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation.

Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).

Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.

Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.

User-friendly data analysis: Analyze sequence data in BaseSpace Sequence Hub with methylation apps designed for biologists.

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 8 samples per run  (high output; based on 40× mean coverage and >90% of target bases covered at ≥10×) 2 x 100 bp (max recommended)
NovaSeq 6000 System Samples per run (dual flow cell): S1: 48, S2: 48, S4: 96 (limited by available index combinations; based on >55 million reads) 2 × 100 bp (max recommended)
HiSeq 2500 System Samples per run: rapid run: 8-12, high output v4: 64 (based on 40× mean coverage and >90% of target bases covered at ≥10×) 2 × 100 bp (max recommended)
HiSeq 3000 System 48 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) 2 × 100 bp (max recommended)
HiSeq 4000 System 96 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) 2 × 100 bp (max recommended)

Product Comparison

TruSeq Methyl Capture EPIC Library Prep Kit Infinium MethylationEPIC Kit
Content Specifications Targets over 3.3 million CpGs. Interrogates biologically important methylome targets. Targets 850,000 CpGs. Quantitatively interrogates selected methylation sites across the genome.
Description Focused interrogation of a set of methylome targets for a balanced, cost-effective option that can support both screening studies and biomarker discovery research objectives. Quantitative interrogation of selected methylation sites across the genome, offering high-throughput research capabilities at a minimal cost per sample.
Input Quantity 500 ng human genomic DNA 250 ng DNA
Method Methylation Sequencing , Targeted DNA Sequencing Methylation Array
Number of Samples Up to 12-plex 8 samples per array
Species Category Human Human
Technology Sequencing Microarray

Method-Specific Workflow Example

 

Supporting Data and Figures

 

This kit requires a PCR enzyme kit such as the Kapa Hifi Uracil+ (Cat No. KK2801), available separately.

Related Products

Infinium MethylationEPIC Kit

Robust methylation profiling microarray with extensive coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.


AmpliSeq for Illumina Transcriptome Human Gene Expression Panel

Targeted panel that measures expression levels of >20,000 human RefSeq genes.


AmpliSeq for Illumina Comprehensive Cancer Panel

Targeted panel investigating the exonic regions of 409 genes with known associations to cancer.


References
  1. Masser DR, Stanford DR, and Freeman WM. Targeted DNA methylation analysis by next-generation sequencing. J Vis Exp. 2015; (96).
  2. Agilent SureSelect MethylSeq target regions. Accessed 14 June 2016.
  3. Roche Nimblegen CpGiant target regions. Accessed 14 June 2016.